TY - JOUR TI - SUMO is a pervasive regulator of meiosis AU - Bhagwat, Nikhil R AU - Owens, Shannon N AU - Ito, Masaru AU - Boinapalli, Jay V AU - Poa, Philip AU - Ditzel, Alexander AU - Kopparapu, Srujan AU - Mahalawat, Meghan AU - Davies, Owen Richard AU - Collins, Sean R AU - Johnson, Jeffrey R AU - Krogan, Nevan J AU - Hunter, Neil A2 - Marston, Adèle L A2 - Cole, Philip A A2 - Marston, Adèle L A2 - Pelisch, Federico A2 - Matos, Joao A2 - Kirkpatrick, Donald S VL - 10 PY - 2021 DA - 2021/01/27 SP - e57720 C1 - eLife 2021;10:e57720 DO - 10.7554/eLife.57720 UR - https://doi.org/10.7554/eLife.57720 AB - Protein modification by SUMO helps orchestrate the elaborate events of meiosis to faithfully produce haploid gametes. To date, only a handful of meiotic SUMO targets have been identified. Here, we delineate a multidimensional SUMO-modified meiotic proteome in budding yeast, identifying 2747 conjugation sites in 775 targets, and defining their relative levels and dynamics. Modified sites cluster in disordered regions and only a minority match consensus motifs. Target identities and modification dynamics imply that SUMOylation regulates all levels of chromosome organization and each step of meiotic prophase I. Execution-point analysis confirms these inferences, revealing functions for SUMO in S-phase, the initiation of recombination, chromosome synapsis and crossing over. K15-linked SUMO chains become prominent as chromosomes synapse and recombine, consistent with roles in these processes. SUMO also modifies ubiquitin, forming hybrid oligomers with potential to modulate ubiquitin signaling. We conclude that SUMO plays diverse and unanticipated roles in regulating meiotic chromosome metabolism. KW - meiosis KW - SUMO KW - Ubiquitin KW - proteomics KW - homologous recombination KW - crossing over JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -