TY - JOUR TI - Inhibition of post-termination ribosome recycling at premature termination codons in UPF1 ATPase mutants AU - Serdar, Lucas D AU - Whiteside, DaJuan L AU - Nock, Sarah L AU - McGrath, David AU - Baker, Kristian E A2 - Manley, James L A2 - Hinnebusch, Alan G A2 - Mühlemann, Oliver VL - 9 PY - 2020 DA - 2020/07/22 SP - e57834 C1 - eLife 2020;9:e57834 DO - 10.7554/eLife.57834 UR - https://doi.org/10.7554/eLife.57834 AB - Recognition and rapid degradation of mRNA harboring premature translation termination codons (PTCs) serves to protect cells from accumulating non-functional and potentially toxic truncated polypeptides. Targeting of PTC-containing transcripts is mediated by the nonsense-mediated mRNA decay (NMD) pathway and requires a conserved set of proteins including UPF1, an RNA helicase whose ATPase activity is essential for NMD. Previously, we identified a functional interaction between the NMD machinery and terminating ribosomes based on 3’ RNA decay fragments that accrue in UPF1 ATPase mutants. Herein, we show that those decay intermediates originate downstream of the PTC and harbor 80S ribosomes that migrate into the mRNA 3’ UTR independent of canonical translation. Accumulation of 3’ RNA decay fragments is determined by both RNA sequence downstream of the PTC and the inactivating mutation within the active site of UPF1. Our data reveal a failure in post-termination ribosome recycling in UPF1 ATPase mutants. KW - nonsense mediated decay KW - UPF1 KW - translation KW - ribosome recycling KW - termination JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -