TY - JOUR TI - Effective dynamics of nucleosome configurations at the yeast PHO5 promoter AU - Wolff, Michael Roland AU - Schmid, Andrea AU - Korber, Philipp AU - Gerland, Ulrich A2 - Barkai, Naama A2 - Gunawardena, Jeremy VL - 10 PY - 2021 DA - 2021/03/05 SP - e58394 C1 - eLife 2021;10:e58394 DO - 10.7554/eLife.58394 UR - https://doi.org/10.7554/eLife.58394 AB - Chromatin dynamics are mediated by remodeling enzymes and play crucial roles in gene regulation, as established in a paradigmatic model, the Saccharomyces cerevisiae PHO5 promoter. However, effective nucleosome dynamics, that is, trajectories of promoter nucleosome configurations, remain elusive. Here, we infer such dynamics from the integration of published single-molecule data capturing multi-nucleosome configurations for repressed to fully active PHO5 promoter states with other existing histone turnover and new chromatin accessibility data. We devised and systematically investigated a new class of ‘regulated on-off-slide’ models simulating global and local nucleosome (dis)assembly and sliding. Only seven of 68,145 models agreed well with all data. All seven models involve sliding and the known central role of the N-2 nucleosome, but regulate promoter state transitions by modulating just one assembly rather than disassembly process. This is consistent with but challenges common interpretations of previous observations at the PHO5 promoter and suggests chromatin opening by binding competition. KW - PHO5 promoter KW - nucleosome dynamics KW - nucleosome configurations KW - chromatin regulation KW - chromatin remodeling JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -