TY - JOUR TI - A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress AU - Stephani, Madlen AU - Picchianti, Lorenzo AU - Gajic, Alexander AU - Beveridge, Rebecca AU - Skarwan, Emilio AU - Sanchez de Medina Hernandez, Victor AU - Mohseni, Azadeh AU - Clavel, Marion AU - Zeng, Yonglun AU - Naumann, Christin AU - Matuszkiewicz, Mateusz AU - Turco, Eleonora AU - Loefke, Christian AU - Li, Baiying AU - Dürnberger, Gerhard AU - Schutzbier, Michael AU - Chen, Hsiao Tieh AU - Abdrakhmanov, Alibek AU - Savova, Adriana AU - Chia, Khong-Sam AU - Djamei, Armin AU - Schaffner, Irene AU - Abel, Steffen AU - Jiang, Liwen AU - Mechtler, Karl AU - Ikeda, Fumiyo AU - Martens, Sascha AU - Clausen, Tim AU - Dagdas, Yasin A2 - Rape, Michael A2 - Pfeffer, Suzanne R VL - 9 PY - 2020 DA - 2020/08/27 SP - e58396 C1 - eLife 2020;9:e58396 DO - 10.7554/eLife.58396 UR - https://doi.org/10.7554/eLife.58396 AB - Eukaryotes have evolved various quality control mechanisms to promote proteostasis in the endoplasmic reticulum (ER). Selective removal of certain ER domains via autophagy (termed as ER-phagy) has emerged as a major quality control mechanism. However, the degree to which ER-phagy is employed by other branches of ER-quality control remains largely elusive. Here, we identify a cytosolic protein, C53, that is specifically recruited to autophagosomes during ER-stress, in both plant and mammalian cells. C53 interacts with ATG8 via a distinct binding epitope, featuring a shuffled ATG8 interacting motif (sAIM). C53 senses proteotoxic stress in the ER lumen by forming a tripartite receptor complex with the ER-associated ufmylation ligase UFL1 and its membrane adaptor DDRGK1. The C53/UFL1/DDRGK1 receptor complex is activated by stalled ribosomes and induces the degradation of internal or passenger proteins in the ER. Consistently, the C53 receptor complex and ufmylation mutants are highly susceptible to ER stress. Thus, C53 forms an ancient quality control pathway that bridges selective autophagy with ribosome-associated quality control in the ER. KW - selective autophagy KW - er-phagy KW - er-quality control KW - ribosome stalling KW - cargo receptor KW - marchantia polymorpha KW - UFMylation JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -