TY - JOUR TI - The leukocyte non-coding RNA landscape in critically ill patients with sepsis AU - Scicluna, Brendon P AU - Uhel, Fabrice AU - van Vught, Lonneke A AU - Wiewel, Maryse A AU - Hoogendijk, Arie J AU - Baessman, Ingelore AU - Franitza, Marek AU - Nürnberg, Peter AU - Horn, Janneke AU - Cremer, Olaf L AU - Bonten, Marc J AU - Schultz, Marcus J AU - van der Poll, Tom AU - Molecular Diagnosis and Risk Stratification in Sepsis (MARS) consortium A2 - Giamarellos-Bourboulis, Evangelos J A2 - van der Meer, Jos WM A2 - Giamarellos-Bourboulis, Evangelos J A2 - Bauer, Michael VL - 9 PY - 2020 DA - 2020/12/11 SP - e58597 C1 - eLife 2020;9:e58597 DO - 10.7554/eLife.58597 UR - https://doi.org/10.7554/eLife.58597 AB - The extent of non-coding RNA alterations in patients with sepsis and their relationship to clinical characteristics, soluble mediators of the host response to infection, as well as an advocated in vivo model of acute systemic inflammation is unknown. Here we obtained whole blood from 156 patients with sepsis and 82 healthy subjects among whom eight were challenged with lipopolysaccharide in a clinically controlled setting (human endotoxemia). Via next-generation microarray analysis of leukocyte RNA we found that long non-coding RNA and, to a lesser extent, small non-coding RNA were significantly altered in sepsis relative to health. Long non-coding RNA expression, but not small non-coding RNA, was largely recapitulated in human endotoxemia. Integrating RNA profiles and plasma protein levels revealed known as well as previously unobserved pathways, including non-sensory olfactory receptor activity. We provide a benchmark dissection of the blood leukocyte ‘regulome’ that can facilitate prioritization of future functional studies. KW - non-coding RNA KW - sepsis KW - leukocyte JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -