TY - JOUR TI - Rdh54/Tid1 inhibits Rad51-Rad54-mediated D-loop formation and limits D-loop length AU - Shah, Shanaya Shital AU - Hartono, Stella AU - Piazza, Aurèle AU - Som, Vanessa AU - Wright, William AU - Chédin, Frédéric AU - Heyer, Wolf-Dietrich A2 - Spies, Maria A2 - Tyler, Jessica K A2 - Malkova, Anna A2 - Deans, Andrew J VL - 9 PY - 2020 DA - 2020/11/13 SP - e59112 C1 - eLife 2020;9:e59112 DO - 10.7554/eLife.59112 UR - https://doi.org/10.7554/eLife.59112 AB - Displacement loops (D-loops) are critical intermediates formed during homologous recombination. Rdh54 (a.k.a. Tid1), a Rad54 paralog in Saccharomyces cerevisiae, is well-known for its role with Dmc1 recombinase during meiotic recombination. Yet contrary to Dmc1, Rdh54/Tid1 is also present in somatic cells where its function is less understood. While Rdh54/Tid1 enhances the Rad51 DNA strand invasion activity in vitro, it is unclear how it interplays with Rad54. Here, we show that Rdh54/Tid1 inhibits D-loop formation by Rad51 and Rad54 in an ATPase-independent manner. Using a novel D-loop Mapping Assay, we further demonstrate that Rdh54/Tid1 uniquely restricts the length of Rad51-Rad54-mediated D-loops. The alterations in D-loop properties appear to be important for cell survival and mating-type switch in haploid yeast. We propose that Rdh54/Tid1 and Rad54 compete for potential binding sites within the Rad51 filament, where Rdh54/Tid1 acts as a physical roadblock to Rad54 translocation, limiting D-loop formation and D-loop length. KW - recombination KW - genome stability KW - dsb repair JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -