TY - JOUR TI - Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase AU - Geymonat, Marco AU - Peng, Qiuran AU - Guo, Zhiang AU - Yu, Zulin AU - Unruh, Jay R AU - Jaspersen, Sue L AU - Segal, Marisa A2 - Lüders, Jens A2 - Akhmanova, Anna VL - 9 PY - 2020 DA - 2020/08/27 SP - e59222 C1 - eLife 2020;9:e59222 DO - 10.7554/eLife.59222 UR - https://doi.org/10.7554/eLife.59222 AB - Asymmetric astral microtubule organization drives the polarized orientation of the S. cerevisiae mitotic spindle and primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud. This model has anticipated analogous centrosome asymmetries featured in self-renewing stem cell divisions. We previously implicated Spc72, the cytoplasmic receptor for the gamma-tubulin nucleation complex, as the most upstream determinant linking SPB age, functional asymmetry and fate. Here we used structured illumination microscopy and biochemical analysis to explore the asymmetric landscape of nucleation sites inherently built into the spindle pathway and under the control of cyclin-dependent kinase (CDK). We show that CDK enforces Spc72 asymmetric docking by phosphorylating Nud1/centriolin. Furthermore, CDK-imposed order in the construction of the new SPB promotes the correct balance of nucleation sites between the nuclear and cytoplasmic faces of the SPB. Together these contributions by CDK inherently link correct SPB morphogenesis, age and fate. KW - spindle KW - polarity KW - asymmetric fate KW - gamma-tubulin KW - cell cycle JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -