TY - JOUR TI - Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila AU - Yu, Shichao AU - Luo, Fangzhou AU - Jin, Li Hua A2 - Shim, Jiwon A2 - Banerjee, Utpal A2 - Waltzer, Lucas VL - 10 PY - 2021 DA - 2021/02/09 SP - e60870 C1 - eLife 2021;10:e60870 DO - 10.7554/eLife.60870 UR - https://doi.org/10.7554/eLife.60870 AB - The hematopoietic system of Drosophila is a powerful genetic model for studying hematopoiesis, and vesicle trafficking is important for signal transduction during various developmental processes; however, its interaction with hematopoiesis is currently largely unknown. In this article, we selected three endosome markers, Rab5, Rab7, and Rab11, that play a key role in membrane trafficking and determined whether they participate in hematopoiesis. Inhibiting Rab5 or Rab11 in hemocytes or the cortical zone (CZ) significantly induced cell overproliferation and lamellocyte formation in circulating hemocytes and lymph glands and disrupted blood cell progenitor maintenance. Lamellocyte formation involves the JNK, Toll, and Ras/EGFR signaling pathways. Notably, lamellocyte formation was also associated with JNK-dependent autophagy. In conclusion, we identified Rab5 and Rab11 as novel regulators of hematopoiesis, and our results advance the understanding of the mechanisms underlying the maintenance of hematopoietic homeostasis as well as the pathology of blood disorders such as leukemia. KW - vesicle trafficking KW - Rab5 KW - Rab11 KW - hematopoiesis KW - lymph gland KW - hemocyte JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -