Cytoneme delivery of sonic hedgehog from ligand-producing cells requires Myosin 10 and a dispatched-BOC/CDON co-receptor complex
- St. Jude Children's Research Hospital, United States
- St Jude Children's Research Hospital, United States
- University of North Carolina School of Medicine, United States
Abstract
Morphogens function in concentration-dependent manners to instruct cell fate during tissue patterning. The cytoneme morphogen transport model posits that specialized filopodia extend between morphogen-sending and responding cells to ensure that appropriate signaling thresholds are achieved. How morphogens are transported along and deployed from cytonemes, how quickly a cytoneme-delivered, receptor-dependent signal is initiated, and whether these processes are conserved across phyla are not known. Herein, we reveal that the actin motor Myosin 10 promotes vesicular transport of Sonic Hedgehog (SHH) morphogen in mouse cell cytonemes, and that SHH morphogen gradient organization is altered in neural tubes of Myo10-/- mice. We demonstrate that cytoneme-mediated deposition of SHH onto receiving cells induces a rapid, receptor-dependent signal response that occurs within seconds of ligand delivery. This activity is dependent upon a novel Dispatched (DISP)-BOC/CDON co-receptor complex that functions in ligand-producing cells to promote cytoneme occurrence and facilitate ligand delivery for signal activation.
Data availability
All data generated or analyzed during the study are included in the manuscript and supporting files. Source files are provided for Figure 2.
Article and author information
Author details
Shondra M Pruett-Miller
Camenzind G Robinson
Funding
National Institute of General Medical Sciences (R35GM122546)
- Stacey K Ogden
National Institute of General Medical Sciences (R01GM134531)
- Richard E Cheney
National Cancer Institute (P30 CA021765)
- Camenzind G Robinson
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Duojia Pan, UT Southwestern Medical Center and HHMI, United States
Ethics
Animal experimentation: The study was performed per recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All animals were handled according to the approved institutional animal care and use committee protocol number 608-100616-10/19 of St. Jude Children's Research Hospital. All effort was made to minimize suffering.
Version history
- Received: July 25, 2020
- Accepted: February 10, 2021
- Accepted Manuscript published: February 11, 2021 (version 1)
- Version of Record published: March 17, 2021 (version 2)
Copyright
© 2021, Hall et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Cytoneme delivery of sonic hedgehog from ligand-producing cells requires Myosin 10 and a dispatched-BOC/CDON co-receptor complex
eLife 10:e61432.
https://doi.org/10.7554/eLife.61432
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