TY - JOUR TI - Rapid adaptation of endocytosis, exocytosis, and eisosomes after an acute increase in membrane tension in yeast cells AU - Lemière, Joël AU - Ren, Yuan AU - Berro, Julien A2 - Malhotra, Vivek A2 - Lamaze, Christophe VL - 10 PY - 2021 DA - 2021/05/13 SP - e62084 C1 - eLife 2021;10:e62084 DO - 10.7554/eLife.62084 UR - https://doi.org/10.7554/eLife.62084 AB - During clathrin-mediated endocytosis (CME) in eukaryotes, actin assembly is required to overcome large membrane tension and turgor pressure. However, the molecular mechanisms by which the actin machinery adapts to varying membrane tension remain unknown. In addition, how cells reduce their membrane tension when they are challenged by hypotonic shocks remains unclear. We used quantitative microscopy to demonstrate that cells rapidly reduce their membrane tension using three parallel mechanisms. In addition to using their cell wall for mechanical protection, yeast cells disassemble eisosomes to buffer moderate changes in membrane tension on a minute time scale. Meanwhile, a temporary reduction in the rate of endocytosis for 2–6 min and an increase in the rate of exocytosis for at least 5 min allow cells to add large pools of membrane to the plasma membrane. We built on these results to submit the cells to abrupt increases in membrane tension and determine that the endocytic actin machinery of fission yeast cells rapidly adapts to perform CME. Our study sheds light on the tight connection between membrane tension regulation, endocytosis, and exocytosis. KW - endocytosis KW - actin KW - exocytosis JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -