TY - JOUR TI - Fine interaction profiling of VemP and mechanisms responsible for its translocation-coupled arrest-cancelation AU - Miyazaki, Ryoji AU - Akiyama, Yoshinori AU - Mori, Hiroyuki A2 - Pfeffer, Suzanne R VL - 9 PY - 2020 DA - 2020/12/15 SP - e62623 C1 - eLife 2020;9:e62623 DO - 10.7554/eLife.62623 UR - https://doi.org/10.7554/eLife.62623 AB - Bacterial cells utilize monitoring substrates, which undergo force-sensitive translation elongation arrest, to feedback-regulate a Sec-related gene. Vibrio alginolyticus VemP controls the expression of SecD/F that stimulates a late step of translocation by undergoing export-regulated elongation arrest. Here, we attempted at delineating the pathway of the VemP nascent-chain interaction with Sec-related factors, and identified the signal recognition particle (SRP) and PpiD (a membrane-anchored periplasmic chaperone) in addition to other translocon components and a ribosomal protein as interacting partners. Our results showed that SRP is required for the membrane-targeting of VemP, whereas PpiD acts cooperatively with SecD/F in the translocation and arrest-cancelation of VemP. We also identified the conserved Arg-85 residue of VemP as a crucial element that confers PpiD-dependence to VemP and plays an essential role in the regulated arrest-cancelation. We propose a scheme of the arrest-cancelation processes of VemP, which likely monitors late steps in the protein translocation pathway. KW - Vibrio KW - protein export KW - nascent chain KW - SecY KW - SecG KW - Ffh JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -