Tuning self-renewal in the Arabidopsis stomatal lineage by hormone and nutrient regulation of asymmetric cell division

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Abstract

Asymmetric and self-renewing divisions build and pattern tissues. In the Arabidopsis stomatal lineage, asymmetric cell divisions, guided by polarly localized cortical proteins, generate most cells on the leaf surface. Systemic and environmental signals modify tissue development, but the mechanisms by which plants incorporate such cues to regulate asymmetric divisions are elusive. In a screen for modulators of cell polarity, we identified CONSTITUTIVE TRIPLE RESPONSE1, a negative regulator of ethylene signaling. We subsequently revealed antagonistic impacts of ethylene and glucose signaling on the self-renewing capacity of stomatal lineage stem-cells. Quantitative analysis of cell polarity and fate dynamics showed that developmental information may be encoded in both the spatial and temporal asymmetries of polarity proteins. These results provide a framework for a mechanistic understanding of how nutritional status and environmental factors tune stem-cell behavior in the stomatal lineage, ultimately enabling flexibility in leaf size and cell-type composition.

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All data generated on analyzed during this study are include in the manuscript and supporting files.

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Yan Gong

Department of Biology, Stanford University, Stanford, United States

Competing interests
No competing interests declared.
Julien Alassimone

Department of Biology, Stanford University, Stanford, United States

Competing interests
No competing interests declared.
Rachel Varnau

Department of Biology, Stanford University, Stanford, United States

Competing interests
No competing interests declared.
Nidhi Sharma

Department of Biology, Stanford University, Stanford, United States

Competing interests
No competing interests declared.

"This ORCID iD identifies the author of this article:" 0000-0002-9725-5338
Lily S Cheung

School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, United States

Competing interests
No competing interests declared.
Dominique C Bergmann

Department of Biology, Stanford University, Stanford, United States

For correspondence
bergmann@stanford.edu

Competing interests
Dominique C Bergmann, Reviewing editor, eLife.

"This ORCID iD identifies the author of this article:" 0000-0003-0873-3543

Funding

Howard Hughes Medical Institute

Dominique C Bergmann

Swiss national science foundation (EPM-PBLAP3-142757)

Julien Alassimone

European Molecular Biology Organization (ALTF-878-2013)

Julien Alassimone

Howard Hughes Medical Institute

Yan Gong

Howard Hughes Medical Institute

Nidhi Sharma

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.