1. Plant Biology

Tuning self-renewal in the Arabidopsis stomatal lineage by hormone and nutrient regulation of asymmetric cell division

  1. Yan Gong
  2. Julien Alassimone
  3. Rachel Varnau
  4. Nidhi Sharma
  5. Lily S Cheung
  6. Dominique C Bergmann  Is a corresponding author
  1. Stanford University, United States
  2. Georgia Institute of Technology, United States
https://doi.org/10.7554/eLife.63335
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  1. Yan Gong
  2. Julien Alassimone
  3. Rachel Varnau
  4. Nidhi Sharma
  5. Lily S Cheung
  6. Dominique C Bergmann
(2021)
Tuning self-renewal in the Arabidopsis stomatal lineage by hormone and nutrient regulation of asymmetric cell division
eLife 10:e63335.
https://doi.org/10.7554/eLife.63335
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Abstract

Asymmetric and self-renewing divisions build and pattern tissues. In the Arabidopsis stomatal lineage, asymmetric cell divisions, guided by polarly localized cortical proteins, generate most cells on the leaf surface. Systemic and environmental signals modify tissue development, but the mechanisms by which plants incorporate such cues to regulate asymmetric divisions are elusive. In a screen for modulators of cell polarity, we identified CONSTITUTIVE TRIPLE RESPONSE1, a negative regulator of ethylene signaling. We subsequently revealed antagonistic impacts of ethylene and glucose signaling on the self-renewing capacity of stomatal lineage stem-cells. Quantitative analysis of cell polarity and fate dynamics showed that developmental information may be encoded in both the spatial and temporal asymmetries of polarity proteins. These results provide a framework for a mechanistic understanding of how nutritional status and environmental factors tune stem-cell behavior in the stomatal lineage, ultimately enabling flexibility in leaf size and cell-type composition.

Data availability

All data generated on analyzed during this study are include in the manuscript and supporting files.

Article and author information

Author details

  1. Yan Gong

    Department of Biology, Stanford University, Stanford, United States
    Competing interests
    No competing interests declared.

  2. Julien Alassimone

    Department of Biology, Stanford University, Stanford, United States
    Competing interests
    No competing interests declared.

  3. Rachel Varnau

    Department of Biology, Stanford University, Stanford, United States
    Competing interests
    No competing interests declared.

  4. Nidhi Sharma

    Department of Biology, Stanford University, Stanford, United States
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-9725-5338

  5. Lily S Cheung

    School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, United States
    Competing interests
    No competing interests declared.

  6. Dominique C Bergmann

    Department of Biology, Stanford University, Stanford, United States
    For correspondence
    bergmann@stanford.edu
    Competing interests
    Dominique C Bergmann, Reviewing editor, eLife.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0873-3543

Funding

Howard Hughes Medical Institute

  • Dominique C Bergmann

Swiss national science foundation (EPM-PBLAP3-142757)

  • Julien Alassimone

European Molecular Biology Organization (ALTF-878-2013)

  • Julien Alassimone

Howard Hughes Medical Institute

  • Yan Gong

Howard Hughes Medical Institute

  • Nidhi Sharma

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Hao Yu, National University of Singapore & Temasek Life Sciences Laboratory, Singapore

Publication history

  1. Received: September 22, 2020
  2. Accepted: March 18, 2021
  3. Accepted Manuscript published: March 19, 2021 (version 1)
  4. Version of Record published: March 30, 2021 (version 2)

Copyright

© 2021, Gong et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Yan Gong
  2. Julien Alassimone
  3. Rachel Varnau
  4. Nidhi Sharma
  5. Lily S Cheung
  6. Dominique C Bergmann
(2021)
Tuning self-renewal in the Arabidopsis stomatal lineage by hormone and nutrient regulation of asymmetric cell division
eLife 10:e63335.
https://doi.org/10.7554/eLife.63335

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