TY - JOUR TI - Single-cell transcriptomes of developing and adult olfactory receptor neurons in Drosophila AU - McLaughlin, Colleen N AU - Brbić, Maria AU - Xie, Qijing AU - Li, Tongchao AU - Horns, Felix AU - Kolluru, Sai Saroja AU - Kebschull, Justus M AU - Vacek, David AU - Xie, Anthony AU - Li, Jiefu AU - Jones, Robert C AU - Leskovec, Jure AU - Quake, Stephen R AU - Luo, Liqun AU - Li, Hongjie A2 - Bellen, Hugo J A2 - VijayRaghavan, K A2 - Barish, Scott VL - 10 PY - 2021 DA - 2021/02/08 SP - e63856 C1 - eLife 2021;10:e63856 DO - 10.7554/eLife.63856 UR - https://doi.org/10.7554/eLife.63856 AB - Recognition of environmental cues is essential for the survival of all organisms. Transcriptional changes occur to enable the generation and function of the neural circuits underlying sensory perception. To gain insight into these changes, we generated single-cell transcriptomes of Drosophila olfactory- (ORNs), thermo-, and hygro-sensory neurons at an early developmental and adult stage using single-cell and single-nucleus RNA sequencing. We discovered that ORNs maintain expression of the same olfactory receptors across development. Using receptor expression and computational approaches, we matched transcriptomic clusters corresponding to anatomically and physiologically defined neuron types across multiple developmental stages. We found that cell-type-specific transcriptomes partly reflected axon trajectory choices in development and sensory modality in adults. We uncovered stage-specific genes that could regulate the wiring and sensory responses of distinct ORN types. Collectively, our data reveal transcriptomic features of sensory neuron biology and provide a resource for future studies of their development and physiology. KW - single-cell RNA-seq KW - single-nucleus RNA-seq KW - olfactory system KW - olfactory receptor neuron KW - olfactory receptor KW - Drosophila JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -