A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA
- Ocean University of China, China
- Shandong University, China
- University of Warwick, United Kingdom
- University of East Anglia, United Kingdom
Abstract
Dimethylsulfoniopropionate (DMSP) is an abundant and ubiquitous organosulfur molecule in marine environments with important roles in global sulfur and nutrient cycling. Diverse DMSP lyases in some algae, bacteria and fungi cleave DMSP to yield gaseous dimethyl sulfide (DMS), an infochemical with important roles in atmospheric chemistry. Here we identified a novel ATP-dependent DMSP lyase, DddX. DddX belongs to the acyl-CoA synthetase superfamily and is distinct from the eight other known DMSP lyases. DddX catalyses the conversion of DMSP to DMS via a two-step reaction: the ligation of DMSP with CoA to form the intermediate DMSP-CoA, which is then cleaved to DMS and acryloyl-CoA. The novel catalytic mechanism was elucidated by structural and biochemical analyses. DddX is found in several Alphaproteobacteria, Gammaproteobacteria and Firmicutes, suggesting that this new DMSP lyase may play an overlooked role in DMSP/DMS cycles.
Data availability
The draft genome sequences of Psychrobacter sp. D2 have been deposited in the National Center for Biotechnology Information (NCBI) Genome database under accession number JACDXZ000000000. All the RNA-seq read data have been deposited in NCBI's sequence read archive (SRA) under project accession number PRJNA646786. The structure of DddX/ATP complex has been deposited in the PDB under the accession code 7CM9.
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The draft genome sequence of Psychrobacter sp.D2NCBI Genome database, JACDXZ000000000.
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RNA-Seq of Psychrobacter sp. D2NCBI's sequence read archive (SRA),PRJNA646786.
Article and author information
Author details
Funding
National Key Research and Development Program of China (2018YFC1406700)
- Yu-Zhong Zhang
National Science Foundation of China (91851205)
- Yu-Zhong Zhang
National Science Foundation of China (31630012)
- Yu-Zhong Zhang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Georg Pohnert, University of Jena, Germany
Version history
- Received: October 15, 2020
- Accepted: May 9, 2021
- Accepted Manuscript published: May 10, 2021 (version 1)
- Version of Record published: May 28, 2021 (version 2)
Copyright
© 2021, Li et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A novel ATP dependent dimethylsulfoniopropionate lyase in bacteria that releases dimethyl sulfide and acryloyl-CoA
eLife 10:e64045.
https://doi.org/10.7554/eLife.64045
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