TY - JOUR TI - A subcellular map of the human kinome AU - Zhang, Haitao AU - Cao, Xiaolei AU - Tang, Mei AU - Zhong, Guoxuan AU - Si, Yuan AU - Li, Haidong AU - Zhu, Feifeng AU - Liao, Qinghua AU - Li, Liuju AU - Zhao, Jianhui AU - Feng, Jia AU - Li, Shuaifeng AU - Wang, Chenliang AU - Kaulich, Manuel AU - Wang, Fangwei AU - Chen, Liangyi AU - Li, Li AU - Xia, Zongping AU - Liang, Tingbo AU - Lu, Huasong AU - Feng, Xin-Hua AU - Zhao, Bin A2 - Cooper, Jonathan A A2 - Hunter, Tony VL - 10 PY - 2021 DA - 2021/05/14 SP - e64943 C1 - eLife 2021;10:e64943 DO - 10.7554/eLife.64943 UR - https://doi.org/10.7554/eLife.64943 AB - The human kinome comprises 538 kinases playing essential functions by catalyzing protein phosphorylation. Annotation of subcellular distribution of the kinome greatly facilitates investigation of normal and disease mechanisms. Here, we present Kinome Atlas (KA), an image-based map of the kinome annotated to 10 cellular compartments. 456 epitope-tagged kinases, representing 85% of the human kinome, were expressed in HeLa cells and imaged by immunofluorescent microscopy under a similar condition. KA revealed kinase family-enriched subcellular localizations and discovered a collection of new kinase localizations at mitochondria, plasma membrane, extracellular space, and other structures. Furthermore, KA demonstrated the role of liquid-liquid phase separation in formation of kinase condensates. Identification of MOK as a mitochondrial kinase revealed its function in cristae dynamics, respiration, and oxidative stress response. Although limited by possible mislocalization due to overexpression or epitope tagging, this subcellular map of the kinome can be used to refine regulatory mechanisms involving protein phosphorylation. KW - kinome KW - subcellular localization KW - phase separation KW - mitochondrial KW - MOK JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -