TY - JOUR TI - A conserved strategy for inducing appendage regeneration in moon jellyfish, Drosophila, and mice AU - Abrams, Michael J AU - Tan, Fayth Hui AU - Li, Yutian AU - Basinger, Ty AU - Heithe, Martin L AU - Sarma, Anish AU - Lee, Iris T AU - Condiotte, Zevin J AU - Raffiee, Misha AU - Dabiri, John O AU - Gold, David A AU - Goentoro, Lea A2 - Courtier-Orgogozo, Virginie A2 - Wittkopp, Patricia J A2 - Courtier-Orgogozo, Virginie A2 - Gazave, Eve VL - 10 PY - 2021 DA - 2021/12/07 SP - e65092 C1 - eLife 2021;10:e65092 DO - 10.7554/eLife.65092 UR - https://doi.org/10.7554/eLife.65092 AB - Can limb regeneration be induced? Few have pursued this question, and an evolutionarily conserved strategy has yet to emerge. This study reports a strategy for inducing regenerative response in appendages, which works across three species that span the animal phylogeny. In Cnidaria, the frequency of appendage regeneration in the moon jellyfish Aurelia was increased by feeding with the amino acid L-leucine and the growth hormone insulin. In insects, the same strategy induced tibia regeneration in adult Drosophila. Finally, in mammals, L-leucine and sucrose administration induced digit regeneration in adult mice, including dramatically from mid-phalangeal amputation. The conserved effect of L-leucine and insulin/sugar suggests a key role for energetic parameters in regeneration induction. The simplicity by which nutrient supplementation can induce appendage regeneration provides a testable hypothesis across animals. KW - appendage regeneration KW - limb regeneration KW - leucine KW - insulin KW - sucrose JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -