TY - JOUR TI - Single-cell analysis of the ventricular-subventricular zone reveals signatures of dorsal and ventral adult neurogenesis AU - Cebrian-Silla, Arantxa AU - Nascimento, Marcos Assis AU - Redmond, Stephanie A AU - Mansky, Benjamin AU - Wu, David AU - Obernier, Kirsten AU - Romero Rodriguez, Ricardo AU - Gonzalez-Granero, Susana AU - García-Verdugo, Jose Manuel AU - Lim, Daniel A AU - Álvarez-Buylla, Arturo A2 - Gleeson, Joseph G A2 - Bronner, Marianne E A2 - van der Kooy, Derek VL - 10 PY - 2021 DA - 2021/07/14 SP - e67436 C1 - eLife 2021;10:e67436 DO - 10.7554/eLife.67436 UR - https://doi.org/10.7554/eLife.67436 AB - The ventricular-subventricular zone (V-SVZ), on the walls of the lateral ventricles, harbors the largest neurogenic niche in the adult mouse brain. Previous work has shown that neural stem/progenitor cells (NSPCs) in different locations within the V-SVZ produce different subtypes of new neurons for the olfactory bulb. The molecular signatures that underlie this regional heterogeneity remain largely unknown. Here, we present a single-cell RNA-sequencing dataset of the adult mouse V-SVZ revealing two populations of NSPCs that reside in largely non-overlapping domains in either the dorsal or ventral V-SVZ. These regional differences in gene expression were further validated using a single-nucleus RNA-sequencing reference dataset of regionally microdissected domains of the V-SVZ and by immunocytochemistry and RNAscope localization. We also identify two subpopulations of young neurons that have gene expression profiles consistent with a dorsal or ventral origin. Interestingly, a subset of genes are dynamically expressed, but maintained, in the ventral or dorsal lineages. The study provides novel markers and territories to understand the region-specific regulation of adult neurogenesis. KW - adult neurogenesis KW - single-cell sequencing KW - olfactory bulb KW - regional differences KW - neuroblast KW - stem cell JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -