The LRR-TM protein PAN-1 interacts with MYRF to promote its nuclear translocation in synaptic remodeling

  1. Shi-Li Xia
  2. Meng Li
  3. Bing Chen
  4. Chao Wang
  5. Yong-Hong Yan
  6. Meng-Qiu Dong
  7. Yingchuan B Qi  Is a corresponding author
  1. School of Life Science and Technology, ShanghaiTech University, China
  2. College of Life and Environmental Sciences, Hangzhou Normal University, China
  3. National Institute of Biological Sciences, China
13 figures, 1 table and 5 additional files

Figures

pan-1 mutants exhibit blocked synaptic rewiring.

(A) Top of list for factors interacting with MYRF-1, MYRF-2, and PAN-1, identified by co-IP and Mass-Spec analysis. The factors are ranked by specificity of the binding. The strains used are myrf-1(j…

Figure 2 with 1 supplement
PAN-1 functions cell-autonomously in synaptic rewiring.

(A) Illustration of pan-1 gene and protein domains. Three pan-1 transcripts are produced via trans-splicing. gk142 deletion eliminates the promoter and all three transcripts. The insertion sites of L…

Figure 2—figure supplement 1
PAN-1 acts cell-autonomously.

(A) Synpatic remodeling defects in pan-1(0) is rescued by flp-13pro-driven pan-1. flp-13pro is specifically expressed in DDs among ventral cord motor neurons, even though it is also expressed in a …

Figure 3 with 2 supplements
PAN-1 localizes on the cell membrane.

(A) Signals of PAN-1GFP in pan-1GFP(ybq47) (knock-in) animals. a, embryo. b, head region of L2 larva. c and d, trunk region of L2 larva with a focus on ventral cord neurons (yellow arrows) and seam …

Figure 3—figure supplement 1
pan-1 expression is temporally regulated during development.

(A) Expression of pan-1 transcription reporter (pan-1pro-NLS::tagRFP(ybqIs138)) at four larval stages. RFP is observed broadly at L1 and L2, but downregulated at L3 in most of tissues except for …

Figure 3—figure supplement 2
LRR region and TMD of PAN-1 are important for synaptic rewiring.

Representative images from pan-1(gk142)/mT1, pan-1(gk142), and pan-1(gk142) carrying transgene unc-25pro-pan-1ΔSP(ybqEx643); unc-25pro-pan-1ΔLRR(ybqEx642); unc-25pro-pan-1ΔTM (ybqEx644); unc-25pro-pa…

Figure 4 with 1 supplement
MYRF localizes on the cell membrane and is cleaved at specific stages.

(A) Illustration of functional domains of MYRF. GFP is inserted after Ala171 within the N-MYRF. (B) MYRF-1GFP signals in animals of early and late L1 imaged using wide-field microscope and sCMOS …

Figure 4—figure supplement 1
MYRF-2 is localized at cell membrane.

(A) MYRF-2GFP signals in myrf-2GFP(ybq46) +(Knock in) animals were detected during larval development. At early L1 (three post-hatch hours) (a–d), GFP signals are detected primarily at cell …

Loss-of-cleavage variant of MYRF stays on cell membrane.

(A) Illustration of loss-of-cleavage MYRF-1 mutant protein encoded by myrf-1S483A K488A GFP(syb1487) allele. With the two critical catalytic residues of ICE domain mutated, MYRF-1 cannot be cleaved, …

Figure 6 with 12 supplements
Analysis of synaptic rewiring in myrf-1 mutants.

Quantifications of the ventral and dorsal synapses in myrf-1 single mutants (A) and myrf-1; myrf-2 double mutants (B). Wild-type control (marker only) is flp-13pro-GFP::rab-3(ybqIs47). The allele …

Figure 6—figure supplement 1
Synaptic rewiring in myrf-1 mutants at 14 hr.

Representative images myrf-1 single mutants at 14 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …

Figure 6—figure supplement 2
Synaptic rewiring in myrf-1 mutants at 16 hr.

Representative images myrf-1 single mutants at 16 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …

Figure 6—figure supplement 3
Synaptic rewiring in myrf-1 mutants at 19 hr.

Representative images myrf-1 single mutants at 19 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …

Figure 6—figure supplement 4
Synaptic rewiring in myrf-1 mutants at 21 hr.

Representative images myrf-1 single mutants at 21 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …

Figure 6—figure supplement 5
Synaptic rewiring in myrf-1 mutants at 24 hr.

Representative images myrf-1 single mutants at 24 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …

Figure 6—figure supplement 6
Synaptic rewiring in myrf-1 mutants at 28 hr.

Representative images myrf-1 single mutants at 28 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …

Figure 6—figure supplement 7
Synaptic rewiring in myrf-1; myrf-2 double mutants at 14 hr.

Representative images myrf-1; myrf-2 double mutants at 14 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …

Figure 6—figure supplement 8
Synaptic rewiring in myrf-1; myrf-2 double mutants at 16 hr.

Representative images myrf-1; myrf-2 double mutants at 16 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …

Figure 6—figure supplement 9
Synaptic rewiring in myrf-1; myrf-2 double mutants at 19 hr.

Representative images myrf-1; myrf-2 double mutants at 19 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …

Figure 6—figure supplement 10
Synaptic rewiring in myrf-1; myrf-2 double mutants at 21 hr.

Representative images myrf-1; myrf-2 double mutants at 20 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …

Figure 6—figure supplement 11
Synaptic rewiring in myrf-1; myrf-2 double mutants at 24 hr.

Representative images myrf-1; myrf-2 double mutants at 24 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …

Figure 6—figure supplement 12
Synaptic rewiring in myrf-1; myrf-2 double mutants at 28 hr.

Representative images myrf-1; myrf-2 double mutants at 28 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …

MYRF and PAN-1 interact through extracellular domains in HEK293T cells.

(A) Illustration of PAN-1 and MYRF protein variants (used in B and C) that were expressed in HEK293T cells, showing predicted topology on membrane. (B) PAN-1 interacted with the non-cytoplasmic …

Figure 8 with 2 supplements
Extracellular region is required for MYRF’s cell-membrane localization and function.

(A) Illustration of truncate MYRF-1 proteins encoded by myrf-11-700 GFP(syb1313) and myrf-11-790 GFP(syb1333), with in-frame deletion of the whole and the part of the extracellular region of MYRF-1, …

Figure 8—figure supplement 1
Comparing the signal intensity in MYRF-11-700 GFP(syb1313) and MYRF-11-790 GFP(syb1333).

MYRF-1GFP(ybq14), MYRF-11-700 GFP(syb1313), and MYRF-11-790 GFP(syb1333) animals were imaged under wide-field microscope. Images of five animals were vertically tiled. The display parameters for …

Figure 8—figure supplement 2
Extracellular domain of MYRF-2 is required for its cell-membrane localization.

(A) Illustration of myrf-21-728 GFP(syb1454) with in-frame deletion of the whole extracellular region of MYRF-2. (B) GFP signals in myrf-21-728 GFP(syb1454) mutants at different post-hatch hours. …

myrf-11-700 GFP(syb1313) exhibits precocious, yet discordant synaptic rewiring and M-cell division.

(A) Percentage of animals showing the particular number of M-cell progenies labeled by hlh-8pro-GFP(ayIs6) at 12, 14, 16 post-hatch hours. (B) Quantification of dorsal synapse number of DDs in …

Figure 10 with 1 supplement
PAN-1 is required for MYRF’s cell-membrane and nuclear localization.

(A) Signals of MYRF-1GFP(ybq14) in pan-1(gk142) animals at different post-hatch hours. No clear GFP signals are detected in pan-1 mutants. White arrow, auto-fluorescence. Images are acquired by …

Figure 10—figure supplement 1
pan-1 is required for cell-membrane localization of MYRF.

(A) No clear MYRF-2GFP(ybq46) signals were detected in pan-1(gk142) animals at various post-hatch hours. Images were taken under wide-field microscope, showing anterior half of each animal. Yellow …

MYRF-PAN-1 interaction is important for synaptic remodeling.

(A) Synaptic remodeling in animals with extracellular domain of MYRF overexpressed. Expressed in transgenes were unc-25pro-SP-MYRF-1Ex.C. (ybqEx752) and unc-25pro-TMD-MYRF-1Ex.C. (ybqEx754). SP-, …

Figure 12 with 2 supplements
N-terminal MYRF alone is insufficient for MYRF’s function.

(A) Illustration of truncated MYRF-1 proteins encoded by myrf-11-482 GFP(syb1491) and myrf-11-656 GFP(syb1468), with in-frame deletion of the whole (starting from cleavage point) and the part …

Figure 12—figure supplement 1
Comparing the signal intensity in MYRF-11-482 GFP(syb1491) and MYRF-11-656 GFP(syb1468).

MYRF-11-482 GFP(syb1491) and MYRF-11-656 GFP(syb1468) animals were imaged under wide-field microscope. Images of five animals were vertically tiled. The display parameters for all images were …

Figure 12—figure supplement 2
Single-copy-transgene of MYRF-11-482 (N-MYRF-1) in DDs does not rescue synaptic rewiring defects.

(A) Synaptic remodeling in myrf-1(ybq6); myrf-2(ybq42) double mutants carrying single-copy transgene unc-47pro-gfp::myrf-1(ybqIs92), unc-47pro-gfp::myrf-11-482(ybq99). unc-47pro is expressed in …

A model for how PAN-1 and MYRF interact to regulate synaptic rewiring.

(A) Illustration of synaptic rewiring in wild type and pan-1(0) mutants. Synapses in DDs are indicated by brown-color dots. (B) PAN-1 and MYRF interaction via extracellular domains is required for …

Tables

Appendix 1—key resources table
Reagent type
(species) or resource
DesignationSource or referenceIdentifiersAdditional information
Strain, strain background (C. elegans)Punc-25-mcherry::rab-3(juIs236) IIYishi Jin labCZ8656
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 IIIthis paperBLW1056
Strain, strain background (C. elegans)+/mT1 II; pan-1(gk142)/mT1 III; Pflp-13-GFP::rab-3(ybqIs46)this paperBLW1115
Strain, strain background (C. elegans)Punc-25-GFP(juIs76)/mT1 II, pan-1(gk142)/mT1 IIIthis paperBLW1104
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b::gfp(ybqIs131)this paperBLW1363
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1a(ybqEx624)this paperBLW1319
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1c(ybqEx626)this paperBLW1321
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 IIIBLW1167
Strain, strain background (C. elegans)pan-1-loxp (syb1217) III; Punc-25-mCherry::Rab-3(juIs236) IIthis paperBLW1646
Strain, strain background (C. elegans)pan-1-loxp (syb1217) III; Punc-25-mCherry::Rab-3(juIs236) III; Punc-25-Cre(tmIs1072)this paperBLW1645
Strain, strain background (C. elegans)myrf-1(ju1121) Punc-25-mCherry::Rab-3(juIs236) II; Pmyrf-1-gfp::myrf-1::flag-myrf-1_3'UTR(ybqEx164)Meng et al., 2017BLW314
Strain, strain background (C. elegans)myrf-2(ybq42) X; Pmyrf-2-gfp::myrf-2::HA(ybqIs128)this paperBLW1348
Strain, strain background (C. elegans)Ppan-1-NLS::tagRFP(ybqIs138)this paperBLW1397
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b (delta LRR) (ybqEx642)this paperBLW1401
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b (delta SP) (ybqEx643)this paperBLW1402
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b (delta TM) (ybqEx644)this paperBLW1403
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b (delta Cyto) (ybqEx645)this paperBLW1404
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236) II; Punc-25-mig-10(1-40)::myrf-1 (681–931)::Flag(ybqEx752)this paperBLW1674
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236) II; Punc-25-nep-2 (1–51)::myrf-1 (681–931)::Flag(ybqEx754)this paperBLW1676
Strain, strain background (C. elegans)gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 IIthis paperPHX1313
Strain, strain background (C. elegans)gfp::myrf-1 (1–790)::3xflag(syb1333)/mIn1 IIthis paperPHX1333
Strain, strain background (C. elegans)gfp::myrf-1 (1–482)::3xflag(syb1491)/mIn1 IIthis paperPHX1491
Strain, strain background (C. elegans)gfp::myrf-1 (1–656)::3xflag(syb1468)/mIn1 IIthis paperPHX1468
Strain, strain background (C. elegans)gfp::myrf-1(S483A,K488A)::3xflag(syb1313)/mIn1 IIthis paperPHX1487
Strain, strain background (C. elegans)gfp::myrf-2 (1–728)::3xflag(syb1454) Xthis paperPHX1454
Strain, strain background (C. elegans)gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1570
Strain, strain background (C. elegans)gfp::myrf-1 (1–790)::3xflag(syb1333)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1571
Strain, strain background (C. elegans)gfp::myrf-1 (1–482)::3xflag(syb1491)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1617
Strain, strain background (C. elegans)gfp::myrf-1 (1–656)::3xflag(syb1468)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1615
Strain, strain background (C. elegans)gfp::myrf-1(S483A,K488A)::3xflag(syb1313)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1616
Strain, strain background (C. elegans)gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1756
Strain, strain background (C. elegans)gfp::myrf-1 (1–790)::3xflag(syb1333)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1779
Strain, strain background (C. elegans)gfp::myrf-1 (1–482)::3xflag(syb1491)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1760
Strain, strain background (C. elegans)gfp::myrf-1 (1–656)::3xflag(syb1468)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1758
Strain, strain background (C. elegans)gfp::myrf-1(S483A,K488A)::3xflag(syb1313)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1759
Strain, strain background (C. elegans)gfp::myrf-1(S483A,K488A)::3xflag(syb1313)/mIn1 II; Pflp-13-mCherry::rab-3(ybqIs1)this paperBLW1791
Strain, strain background (C. elegans)gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1)this paperBLW1833
Strain, strain background (C. elegans)gfp::myrf-1 (1–790)::3xflag(syb1333)/mIn1 II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1)this paperBLW1834
Strain, strain background (C. elegans)gfp::myrf-1 (1–482)::3xflag(syb1491)/mIn1 II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1)this paperBLW1835
Strain, strain background (C. elegans)gfp::myrf-1 (1–656)::3xflag(syb1468)/mIn1 II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1)this paperBLW1836
Strain, strain background (C. elegans)pan-1::gfp(ybq47) IIIthis paperBLW1258
Strain, strain background (C. elegans)pan-1::gfp(ybq47) III; glo-1(zu391) Xthis paperBLW1839
Strain, strain background (C. elegans)pan-1::gfp(ybq47) III; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1)this paperBLW1878
Strain, strain background (C. elegans)gfp::myrf-1::3xflag(ybq14) II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1)this paperBLW1854
Strain, strain background (C. elegans)gfp::myrf-1(ybq10) IIMeng et al., 2017BLW831
Strain, strain background (C. elegans)gfp::myrf-1::3xFlag(ybq14) IIMeng et al., 2017BLW889
Strain, strain background (C. elegans)gfp::myrf-2(ybq46) XMeng et al., 2017BLW1111
Strain, strain background (C. elegans)gfp::myrf-1(ybq14)/mT1 II; pan-1(gk142)/mT1 IIIthis paperBLW1465
Strain, strain background (C. elegans)gfp::myrf-1::3xflag(ybq14)/mT1 II; pan-1(gk142)/mT1 IIIthis paperBLW1166
Strain, strain background (C. elegans)gfp::myrf-1::3xflag(ybq14)/mT1 II; pan-1(gk142)/mT1 III; Pscm-pan-1b::mCherry(ybqEx778)this paperBLW1731
Strain, strain background (C. elegans)Pflp-13-GFP::rab-3(ybqIs47) IVthis paperBLW1419
Strain, strain background (C. elegans)myrf-1(ju1121)/mIn1 II; pan-1::GFP(ybq47) IIIthis paperBLW1654
Strain, strain background (C. elegans)gfp::myrf-1::3xflag(ybq14)/mT1 II; pan-1(gk142)/mT1 IIIthis paperBLW1883
Strain, strain background (C. elegans)gfp::myrf-1 (1–700)(syb1313)/mT1 II; pan-1(gk142)/mT1 IIIthis paperBLW1805
Strain, strain background (C. elegans)gfp::myrf-1 (1–790)(syb1333)/mT1 II; pan-1(gk142)/mT1 IIIthis paperBLW1806
Strain, strain background (C. elegans)Pflp-13-GFP::rab-3(ybqIs47) III; Punc-25-pan-1(1-G513)::mCherry::KDEL(ybqEx815)this paperBLW1881
Strain, strain background (C. elegans)Pflp-13-GFP::rab-3(ybqIs47) III; Punc-25-pan-1(1-G513)::mCherry::KDEL(ybqEx817)this paperBLW1890
Strain, strain background (C. elegans)gfp::myrf-1::3xFlag(ybq14) II; Punc-25-pan-1(1-G513)::mCherry::KDEL(ybqEx807)this paperBLW1842
Strain, strain background (C. elegans)gfp::myrf-1::3xFlag(ybq14) II; Pscm-Pan-1(1-G513)::mCherry::KDEL(ybqEx820)this paperBLW1895
Strain, strain background (C. elegans)Punc-25-mCherry::Rab-3(juIs236)/mT1 II; pan-1(gk142)/mT1 III; Pflp-13-pan-1b:gfp(ybqEx821)this paperBLW1900
Strain, strain background (C. elegans)pan-1(loxP)(syb1217) III; Pflp-13-gfp(juIs145)/Punc-25-mCherry::Rab-3(juIs236) IIthis paperBLW1825
Strain, strain background (C. elegans)pan-1(loxP)(syb1217) III; Pflp-13-gfp(juIs145)/Punc-25-mCherry::Rab-3(juIs236) II; Punc-25-Cre(tmIs1072)this paperBLW1784
Strain, strain background (C. elegans)Pflp-13-GFP::rab-3(ybqIs47) III; Phlh-8-GFP(ayIs6) Xthis paperBLW1874
Strain, strain background (C. elegans)Pflp-13-GFP::rab-3(ybqIs47) III; myrf-2(ybq42) Phlh-8-GFP(ayIs6) Xthis paperBLW1875
Strain, strain background (C. elegans)gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) III; Phlh-8-GFP(ayIs6) Xthis paperBLW1843
Strain, strain background (C. elegans)gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) III; myrf-2(ybq42) Phlh-8-GFP(ayIs6) Xthis paperBLW1848
Strain, strain background (C. elegans)myrf-1(ybq6)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) III; Punc-47-gfp::myrf-1::flag, cb-unc-119(+)(ybqIs92(Si)) IVthis paperBLW1781
Strain, strain background (C. elegans)myrf-1(ybq6)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) III; Punc-47-gfp::myrf-1 (1–482)::flag, cb-unc-119(+)(ybqIs99(Si)) IVthis paperBLW1782
Strain, strain background (C. elegans)myrf-1(ybq6)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IIIthis paperBLW1008
Strain, strain background (C. elegans)myrf-1(ybq6)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IIIMeng et al., 2017BLW827

Additional files

Supplementary file 1

Complete list for factors interacting with MYRF-1 in IP-MS analysis (in Figure 1A).

https://cdn.elifesciences.org/articles/67628/elife-67628-supp1-v1.xlsx
Supplementary file 2

Complete list for factors interacting with MYRF-2 in IP-MS analysis (in Figure 1A).

https://cdn.elifesciences.org/articles/67628/elife-67628-supp2-v1.xlsx
Supplementary file 3

Complete list for factors interacting with PAN-1 in IP-MS analysis (in Figure 1A).

https://cdn.elifesciences.org/articles/67628/elife-67628-supp3-v1.xlsx
Supplementary file 4

p-values for comparison between all genotypes in Figure 6.

https://cdn.elifesciences.org/articles/67628/elife-67628-supp4-v1.xlsx
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https://cdn.elifesciences.org/articles/67628/elife-67628-transrepform-v1.docx

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