The LRR-TM protein PAN-1 interacts with MYRF to promote its nuclear translocation in synaptic remodeling
- School of Life Science and Technology, ShanghaiTech University, China
- College of Life and Environmental Sciences, Hangzhou Normal University, China
- National Institute of Biological Sciences, China
Figures
Figure 1
pan-1 mutants exhibit blocked synaptic rewiring.
(A) Top of list for factors interacting with MYRF-1, MYRF-2, and PAN-1, identified by co-IP and Mass-Spec analysis. The factors are ranked by specificity of the binding. The strains used are myrf-1(j…
Figure 2 with 1 supplement
PAN-1 functions cell-autonomously in synaptic rewiring.
(A) Illustration of pan-1 gene and protein domains. Three pan-1 transcripts are produced via trans-splicing. gk142 deletion eliminates the promoter and all three transcripts. The insertion sites of L…
Figure 2—figure supplement 1
PAN-1 acts cell-autonomously.
(A) Synpatic remodeling defects in pan-1(0) is rescued by flp-13pro-driven pan-1. flp-13pro is specifically expressed in DDs among ventral cord motor neurons, even though it is also expressed in a …
Figure 3 with 2 supplements
PAN-1 localizes on the cell membrane.
(A) Signals of PAN-1GFP in pan-1GFP(ybq47) (knock-in) animals. a, embryo. b, head region of L2 larva. c and d, trunk region of L2 larva with a focus on ventral cord neurons (yellow arrows) and seam …
Figure 3—figure supplement 1
pan-1 expression is temporally regulated during development.
(A) Expression of pan-1 transcription reporter (pan-1pro-NLS::tagRFP(ybqIs138)) at four larval stages. RFP is observed broadly at L1 and L2, but downregulated at L3 in most of tissues except for …
Figure 3—figure supplement 2
LRR region and TMD of PAN-1 are important for synaptic rewiring.
Representative images from pan-1(gk142)/mT1, pan-1(gk142), and pan-1(gk142) carrying transgene unc-25pro-pan-1ΔSP(ybqEx643); unc-25pro-pan-1ΔLRR(ybqEx642); unc-25pro-pan-1ΔTM (ybqEx644); unc-25pro-pa…
Figure 4 with 1 supplement
MYRF localizes on the cell membrane and is cleaved at specific stages.
(A) Illustration of functional domains of MYRF. GFP is inserted after Ala171 within the N-MYRF. (B) MYRF-1GFP signals in animals of early and late L1 imaged using wide-field microscope and sCMOS …
Figure 4—figure supplement 1
MYRF-2 is localized at cell membrane.
(A) MYRF-2GFP signals in myrf-2GFP(ybq46) +(Knock in) animals were detected during larval development. At early L1 (three post-hatch hours) (a–d), GFP signals are detected primarily at cell …
Figure 5
Loss-of-cleavage variant of MYRF stays on cell membrane.
(A) Illustration of loss-of-cleavage MYRF-1 mutant protein encoded by myrf-1S483A K488A GFP(syb1487) allele. With the two critical catalytic residues of ICE domain mutated, MYRF-1 cannot be cleaved, …
Figure 6 with 12 supplements
Analysis of synaptic rewiring in myrf-1 mutants.
Quantifications of the ventral and dorsal synapses in myrf-1 single mutants (A) and myrf-1; myrf-2 double mutants (B). Wild-type control (marker only) is flp-13pro-GFP::rab-3(ybqIs47). The allele …
Figure 6—figure supplement 1
Synaptic rewiring in myrf-1 mutants at 14 hr.
Representative images myrf-1 single mutants at 14 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …
Figure 6—figure supplement 2
Synaptic rewiring in myrf-1 mutants at 16 hr.
Representative images myrf-1 single mutants at 16 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …
Figure 6—figure supplement 3
Synaptic rewiring in myrf-1 mutants at 19 hr.
Representative images myrf-1 single mutants at 19 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …
Figure 6—figure supplement 4
Synaptic rewiring in myrf-1 mutants at 21 hr.
Representative images myrf-1 single mutants at 21 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …
Figure 6—figure supplement 5
Synaptic rewiring in myrf-1 mutants at 24 hr.
Representative images myrf-1 single mutants at 24 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …
Figure 6—figure supplement 6
Synaptic rewiring in myrf-1 mutants at 28 hr.
Representative images myrf-1 single mutants at 28 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All animals …
Figure 6—figure supplement 7
Synaptic rewiring in myrf-1; myrf-2 double mutants at 14 hr.
Representative images myrf-1; myrf-2 double mutants at 14 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …
Figure 6—figure supplement 8
Synaptic rewiring in myrf-1; myrf-2 double mutants at 16 hr.
Representative images myrf-1; myrf-2 double mutants at 16 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …
Figure 6—figure supplement 9
Synaptic rewiring in myrf-1; myrf-2 double mutants at 19 hr.
Representative images myrf-1; myrf-2 double mutants at 19 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …
Figure 6—figure supplement 10
Synaptic rewiring in myrf-1; myrf-2 double mutants at 21 hr.
Representative images myrf-1; myrf-2 double mutants at 20 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …
Figure 6—figure supplement 11
Synaptic rewiring in myrf-1; myrf-2 double mutants at 24 hr.
Representative images myrf-1; myrf-2 double mutants at 24 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …
Figure 6—figure supplement 12
Synaptic rewiring in myrf-1; myrf-2 double mutants at 28 hr.
Representative images myrf-1; myrf-2 double mutants at 28 post-hatch hours. The allele genotypes of each mutant are indicated in the figure. Five ventral and dorsal cords are vertically tiled. All …
Figure 7
MYRF and PAN-1 interact through extracellular domains in HEK293T cells.
(A) Illustration of PAN-1 and MYRF protein variants (used in B and C) that were expressed in HEK293T cells, showing predicted topology on membrane. (B) PAN-1 interacted with the non-cytoplasmic …
Figure 8 with 2 supplements
Extracellular region is required for MYRF’s cell-membrane localization and function.
(A) Illustration of truncate MYRF-1 proteins encoded by myrf-11-700 GFP(syb1313) and myrf-11-790 GFP(syb1333), with in-frame deletion of the whole and the part of the extracellular region of MYRF-1, …
Figure 8—figure supplement 1
Comparing the signal intensity in MYRF-11-700 GFP(syb1313) and MYRF-11-790 GFP(syb1333).
MYRF-1GFP(ybq14), MYRF-11-700 GFP(syb1313), and MYRF-11-790 GFP(syb1333) animals were imaged under wide-field microscope. Images of five animals were vertically tiled. The display parameters for …
Figure 8—figure supplement 2
Extracellular domain of MYRF-2 is required for its cell-membrane localization.
(A) Illustration of myrf-21-728 GFP(syb1454) with in-frame deletion of the whole extracellular region of MYRF-2. (B) GFP signals in myrf-21-728 GFP(syb1454) mutants at different post-hatch hours. …
Figure 9
myrf-11-700 GFP(syb1313) exhibits precocious, yet discordant synaptic rewiring and M-cell division.
(A) Percentage of animals showing the particular number of M-cell progenies labeled by hlh-8pro-GFP(ayIs6) at 12, 14, 16 post-hatch hours. (B) Quantification of dorsal synapse number of DDs in …
Figure 10 with 1 supplement
PAN-1 is required for MYRF’s cell-membrane and nuclear localization.
(A) Signals of MYRF-1GFP(ybq14) in pan-1(gk142) animals at different post-hatch hours. No clear GFP signals are detected in pan-1 mutants. White arrow, auto-fluorescence. Images are acquired by …
Figure 10—figure supplement 1
pan-1 is required for cell-membrane localization of MYRF.
(A) No clear MYRF-2GFP(ybq46) signals were detected in pan-1(gk142) animals at various post-hatch hours. Images were taken under wide-field microscope, showing anterior half of each animal. Yellow …
Figure 11
MYRF-PAN-1 interaction is important for synaptic remodeling.
(A) Synaptic remodeling in animals with extracellular domain of MYRF overexpressed. Expressed in transgenes were unc-25pro-SP-MYRF-1Ex.C. (ybqEx752) and unc-25pro-TMD-MYRF-1Ex.C. (ybqEx754). SP-, …
Figure 12 with 2 supplements
N-terminal MYRF alone is insufficient for MYRF’s function.
(A) Illustration of truncated MYRF-1 proteins encoded by myrf-11-482 GFP(syb1491) and myrf-11-656 GFP(syb1468), with in-frame deletion of the whole (starting from cleavage point) and the part …
Figure 12—figure supplement 1
Comparing the signal intensity in MYRF-11-482 GFP(syb1491) and MYRF-11-656 GFP(syb1468).
MYRF-11-482 GFP(syb1491) and MYRF-11-656 GFP(syb1468) animals were imaged under wide-field microscope. Images of five animals were vertically tiled. The display parameters for all images were …
Figure 12—figure supplement 2
Single-copy-transgene of MYRF-11-482 (N-MYRF-1) in DDs does not rescue synaptic rewiring defects.
(A) Synaptic remodeling in myrf-1(ybq6); myrf-2(ybq42) double mutants carrying single-copy transgene unc-47pro-gfp::myrf-1(ybqIs92), unc-47pro-gfp::myrf-11-482(ybq99). unc-47pro is expressed in …
Figure 13
A model for how PAN-1 and MYRF interact to regulate synaptic rewiring.
(A) Illustration of synaptic rewiring in wild type and pan-1(0) mutants. Synapses in DDs are indicated by brown-color dots. (B) PAN-1 and MYRF interaction via extracellular domains is required for …
Tables
Appendix 1—key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (C. elegans) | Punc-25-mcherry::rab-3(juIs236) II | Yishi Jin lab | CZ8656 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III | this paper | BLW1056 | |
| Strain, strain background (C. elegans) | +/mT1 II; pan-1(gk142)/mT1 III; Pflp-13-GFP::rab-3(ybqIs46) | this paper | BLW1115 | |
| Strain, strain background (C. elegans) | Punc-25-GFP(juIs76)/mT1 II, pan-1(gk142)/mT1 III | this paper | BLW1104 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b::gfp(ybqIs131) | this paper | BLW1363 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1a(ybqEx624) | this paper | BLW1319 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1c(ybqEx626) | this paper | BLW1321 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III | BLW1167 | ||
| Strain, strain background (C. elegans) | pan-1-loxp (syb1217) III; Punc-25-mCherry::Rab-3(juIs236) II | this paper | BLW1646 | |
| Strain, strain background (C. elegans) | pan-1-loxp (syb1217) III; Punc-25-mCherry::Rab-3(juIs236) III; Punc-25-Cre(tmIs1072) | this paper | BLW1645 | |
| Strain, strain background (C. elegans) | myrf-1(ju1121) Punc-25-mCherry::Rab-3(juIs236) II; Pmyrf-1-gfp::myrf-1::flag-myrf-1_3'UTR(ybqEx164) | Meng et al., 2017 | BLW314 | |
| Strain, strain background (C. elegans) | myrf-2(ybq42) X; Pmyrf-2-gfp::myrf-2::HA(ybqIs128) | this paper | BLW1348 | |
| Strain, strain background (C. elegans) | Ppan-1-NLS::tagRFP(ybqIs138) | this paper | BLW1397 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b (delta LRR) (ybqEx642) | this paper | BLW1401 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b (delta SP) (ybqEx643) | this paper | BLW1402 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b (delta TM) (ybqEx644) | this paper | BLW1403 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II, pan-1(gk142)/mT1 III; Punc-25-pan-1b (delta Cyto) (ybqEx645) | this paper | BLW1404 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236) II; Punc-25-mig-10(1-40)::myrf-1 (681–931)::Flag(ybqEx752) | this paper | BLW1674 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236) II; Punc-25-nep-2 (1–51)::myrf-1 (681–931)::Flag(ybqEx754) | this paper | BLW1676 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II | this paper | PHX1313 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–790)::3xflag(syb1333)/mIn1 II | this paper | PHX1333 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–482)::3xflag(syb1491)/mIn1 II | this paper | PHX1491 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–656)::3xflag(syb1468)/mIn1 II | this paper | PHX1468 | |
| Strain, strain background (C. elegans) | gfp::myrf-1(S483A,K488A)::3xflag(syb1313)/mIn1 II | this paper | PHX1487 | |
| Strain, strain background (C. elegans) | gfp::myrf-2 (1–728)::3xflag(syb1454) X | this paper | PHX1454 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1570 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–790)::3xflag(syb1333)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1571 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–482)::3xflag(syb1491)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1617 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–656)::3xflag(syb1468)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1615 | |
| Strain, strain background (C. elegans) | gfp::myrf-1(S483A,K488A)::3xflag(syb1313)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1616 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1756 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–790)::3xflag(syb1333)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1779 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–482)::3xflag(syb1491)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1760 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–656)::3xflag(syb1468)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1758 | |
| Strain, strain background (C. elegans) | gfp::myrf-1(S483A,K488A)::3xflag(syb1313)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1759 | |
| Strain, strain background (C. elegans) | gfp::myrf-1(S483A,K488A)::3xflag(syb1313)/mIn1 II; Pflp-13-mCherry::rab-3(ybqIs1) | this paper | BLW1791 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1) | this paper | BLW1833 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–790)::3xflag(syb1333)/mIn1 II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1) | this paper | BLW1834 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–482)::3xflag(syb1491)/mIn1 II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1) | this paper | BLW1835 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–656)::3xflag(syb1468)/mIn1 II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1) | this paper | BLW1836 | |
| Strain, strain background (C. elegans) | pan-1::gfp(ybq47) III | this paper | BLW1258 | |
| Strain, strain background (C. elegans) | pan-1::gfp(ybq47) III; glo-1(zu391) X | this paper | BLW1839 | |
| Strain, strain background (C. elegans) | pan-1::gfp(ybq47) III; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1) | this paper | BLW1878 | |
| Strain, strain background (C. elegans) | gfp::myrf-1::3xflag(ybq14) II; glo-1(zu391) X; Pflp-13-mCherry::rab-3(ybqIs1) | this paper | BLW1854 | |
| Strain, strain background (C. elegans) | gfp::myrf-1(ybq10) II | Meng et al., 2017 | BLW831 | |
| Strain, strain background (C. elegans) | gfp::myrf-1::3xFlag(ybq14) II | Meng et al., 2017 | BLW889 | |
| Strain, strain background (C. elegans) | gfp::myrf-2(ybq46) X | Meng et al., 2017 | BLW1111 | |
| Strain, strain background (C. elegans) | gfp::myrf-1(ybq14)/mT1 II; pan-1(gk142)/mT1 III | this paper | BLW1465 | |
| Strain, strain background (C. elegans) | gfp::myrf-1::3xflag(ybq14)/mT1 II; pan-1(gk142)/mT1 III | this paper | BLW1166 | |
| Strain, strain background (C. elegans) | gfp::myrf-1::3xflag(ybq14)/mT1 II; pan-1(gk142)/mT1 III; Pscm-pan-1b::mCherry(ybqEx778) | this paper | BLW1731 | |
| Strain, strain background (C. elegans) | Pflp-13-GFP::rab-3(ybqIs47) IV | this paper | BLW1419 | |
| Strain, strain background (C. elegans) | myrf-1(ju1121)/mIn1 II; pan-1::GFP(ybq47) III | this paper | BLW1654 | |
| Strain, strain background (C. elegans) | gfp::myrf-1::3xflag(ybq14)/mT1 II; pan-1(gk142)/mT1 III | this paper | BLW1883 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–700)(syb1313)/mT1 II; pan-1(gk142)/mT1 III | this paper | BLW1805 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–790)(syb1333)/mT1 II; pan-1(gk142)/mT1 III | this paper | BLW1806 | |
| Strain, strain background (C. elegans) | Pflp-13-GFP::rab-3(ybqIs47) III; Punc-25-pan-1(1-G513)::mCherry::KDEL(ybqEx815) | this paper | BLW1881 | |
| Strain, strain background (C. elegans) | Pflp-13-GFP::rab-3(ybqIs47) III; Punc-25-pan-1(1-G513)::mCherry::KDEL(ybqEx817) | this paper | BLW1890 | |
| Strain, strain background (C. elegans) | gfp::myrf-1::3xFlag(ybq14) II; Punc-25-pan-1(1-G513)::mCherry::KDEL(ybqEx807) | this paper | BLW1842 | |
| Strain, strain background (C. elegans) | gfp::myrf-1::3xFlag(ybq14) II; Pscm-Pan-1(1-G513)::mCherry::KDEL(ybqEx820) | this paper | BLW1895 | |
| Strain, strain background (C. elegans) | Punc-25-mCherry::Rab-3(juIs236)/mT1 II; pan-1(gk142)/mT1 III; Pflp-13-pan-1b:gfp(ybqEx821) | this paper | BLW1900 | |
| Strain, strain background (C. elegans) | pan-1(loxP)(syb1217) III; Pflp-13-gfp(juIs145)/Punc-25-mCherry::Rab-3(juIs236) II | this paper | BLW1825 | |
| Strain, strain background (C. elegans) | pan-1(loxP)(syb1217) III; Pflp-13-gfp(juIs145)/Punc-25-mCherry::Rab-3(juIs236) II; Punc-25-Cre(tmIs1072) | this paper | BLW1784 | |
| Strain, strain background (C. elegans) | Pflp-13-GFP::rab-3(ybqIs47) III; Phlh-8-GFP(ayIs6) X | this paper | BLW1874 | |
| Strain, strain background (C. elegans) | Pflp-13-GFP::rab-3(ybqIs47) III; myrf-2(ybq42) Phlh-8-GFP(ayIs6) X | this paper | BLW1875 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) III; Phlh-8-GFP(ayIs6) X | this paper | BLW1843 | |
| Strain, strain background (C. elegans) | gfp::myrf-1 (1–700)::3xflag(syb1313)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) III; myrf-2(ybq42) Phlh-8-GFP(ayIs6) X | this paper | BLW1848 | |
| Strain, strain background (C. elegans) | myrf-1(ybq6)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) III; Punc-47-gfp::myrf-1::flag, cb-unc-119(+)(ybqIs92(Si)) IV | this paper | BLW1781 | |
| Strain, strain background (C. elegans) | myrf-1(ybq6)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) III; Punc-47-gfp::myrf-1 (1–482)::flag, cb-unc-119(+)(ybqIs99(Si)) IV | this paper | BLW1782 | |
| Strain, strain background (C. elegans) | myrf-1(ybq6)/mIn1 II; myrf-2(ybq42) X; Pflp-13-GFP::rab-3(ybqIs47) III | this paper | BLW1008 | |
| Strain, strain background (C. elegans) | myrf-1(ybq6)/mIn1 II; Pflp-13-GFP::rab-3(ybqIs47) III | Meng et al., 2017 | BLW827 |
Additional files
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Supplementary file 1
Complete list for factors interacting with MYRF-1 in IP-MS analysis (in Figure 1A).
- https://cdn.elifesciences.org/articles/67628/elife-67628-supp1-v1.xlsx
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Supplementary file 2
Complete list for factors interacting with MYRF-2 in IP-MS analysis (in Figure 1A).
- https://cdn.elifesciences.org/articles/67628/elife-67628-supp2-v1.xlsx
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Supplementary file 3
Complete list for factors interacting with PAN-1 in IP-MS analysis (in Figure 1A).
- https://cdn.elifesciences.org/articles/67628/elife-67628-supp3-v1.xlsx
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Supplementary file 4
p-values for comparison between all genotypes in Figure 6.
- https://cdn.elifesciences.org/articles/67628/elife-67628-supp4-v1.xlsx
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Transparent reporting form
- https://cdn.elifesciences.org/articles/67628/elife-67628-transrepform-v1.docx
