Genetic reagent (Mus musculus) | C57BL/6 J | The Jackson Laboratory | RRID:IMSR_JAX:000664 | |
Genetic reagent (Mus musculus) | EIIa-Cre (FVB/N-Tg(EIIa-cre)C5379Lmgd/J) | The Jackson Laboratory | RRID:IMSR_JAX:003724 | |
Genetic reagent (Mus musculus) | B6.SJL-Ptprca/ BoyAiTac (CD45.1) | The Jackson Laboratory | RRID:IMSR_JAX:002014 | |
Genetic reagent (Mus musculus) | RpS12flox/flox | This study | Rps12em1Nbakr MGI:6388411 | Generated for this study |
Genetic reagent (Mus musculus) | Scl-Cre-ER | The Jackson Laboratory | RRID:IMSR_JAX:037466 | |
Genetic reagent (Mus musculus) | RpS12flox/+; Scl-Cre-ER | This study | | Generated for this study |
Antibody | Rat monoclonal CD16/CD32 | BD biosciences | Cat # 553142 | Clone: 2.4G2 (1 µl per 107 cells) |
Antibody | APC-Cy7 Rat anti-mouse monoclonal Gr1 | BD biosciences | Cat # 557661 | Clone: RB6-8C5 (1 µl per 107 cells) |
Antibody | PE rat anti-mouse monoclonal Mac1 | BioLegend | Cat # 101208 | Clone: M1/70 (1 µl per 107 cells) |
Antibody | Alexa Fluor 700 Rat anti-Mouse monoclonal B220 | BD biosciences | Cat # 557957 | Clone: RA3-6B2 (1 µl per 107 cells) |
Antibody | APC Rat Anti-Mouse monoclonal Thy1.2 | BD biosciences | Cat # 553007 | Clone: 53–2.1 (1 µl per 107 cells) |
Antibody | FITC Mouse Anti-Mouse monoclonal CD45.1 | BD biosciences | Cat # 553775 | Clone: A20 (1 µl per 107 cells) |
Antibody | PE/Dazzle 594 mouse anti-mouse monoclonal CD45.2 | BioLegend | Cat # 109845 | Clone:104 (1 µl per 107 cells) |
Antibody | CD3e hamster monoclonal, Biotin, | eBioscience | Cat # 13003182 | Clone: 145–2 C11 (1 µl per 107 cells) |
Antibody | CD4 rat Monoclonal, Biotin, | eBioscience | Cat #13004182 | Clone: GK1.5 (1 µl per 107 cells) |
Antibody | CD8a rat Monoclonal, Biotin, | eBioscience | Cat # 13008182 | Clone: 53–6.7 (1 µl per 107 cells) |
Antibody | Gr1 rat Monoclonal, Biotin, | eBioscience | Cat # 13593182 | Clone: RB68C5 (1 µl per 107 cells) |
Antibody | B220 rat Monoclonal, Biotin, | eBioscience | Cat # 13045282 | Clone: RA3-6B2 (1 µl per 107 cells) |
Antibody | CD19 mouse Monoclonal, Biotin, | eBioscience | Cat # 13019182 | Clone: MB 19–1 (1 µl per 107 cells) |
Antibody | Ter119 rat Monoclonal, Biotin, | eBioscience | Cat # 13592182 | Clone: TER119 (1 µl per 107 cells) |
Antibody | PE/Cyanine7 rat anti-mouse monoclonal Sca-1 | BioLegend | Cat #108114 | Clone: D7 (1 µl per 107 cells) |
Antibody | APC Rat Anti-Mouse monoclonal cKit | BD Biosciences | Cat #553356 | Clone:2B8 (1 µl per 107 cells) |
Antibody | PE Rat Anti-Mouse monoclonal FCRg | Thermo Fisher | Cat # 12-0161-82 | Clone: 93 (1 µl per 107 cells) |
Antibody | eFluor 450 Rat Anti-Mouse monoclonal CD34 | Thermo Fisher | Cat #48-0341-80 | Clone:RAM34 (1 µl per 107 cells) |
Antibody | Brilliant Violet 421 Rat anti-Mouse monoclonal CD150 | BD Biosciences | Cat #562811 | Clone: Q38-480 (1 µl per 107 cells) |
Antibody | Alexa Fluor 700 hamster anti-mouse monoclonal CD48 | BioLegend | Cat #103426 | Clone: HM48-1 (1 µl per 107 cells) |
Antibody | PE/Cyanine5 Rat anti-mouse monoclonal Flk-2 | BioLegend | Cat #135312 | Clone: A2F10 (1 µl per 107 cells) |
Antibody | Brilliant Violet 605 mouse anti-mouse monoclonal CD45.2 | BioLegend | Cat #109841 | Clone: 104 (1 µl per 107 cells) |
Antibody | PerCP/Cyanine5.5 rat anti-mouse monoclonal IL-7Rα | BioLegend | Cat #135021 | Clone: A7R34 (1 µl per 107 cells) |
Other | Streptavidin-APC-Cy7 | BD Biosciences | Cat # 554063 | second-step reagent for cells stained with biotinylated primary antibodies |
Antibody | PE anti-mouse monoclonal Flk-2 | BioLegend | Cat # 135306 | Clone: A2F10 (1 µl per 107 cells) |
Commercial assay, kit | FITC Annexin V | BD Biosciences | Cat # 560931 | Apoptosis detection: Phosphatidylserine probe |
Commercial assay, kit | Propidium Iodide | BD Biosciences | Cat # 556463 | Apoptosis detection: Staining Solution |
Antibody | pS6 (Ser235/236) Alexa 488 rabbit monoclonal | Cell Signaling | Cat # 4803 S | Clone: D57.2.2E ICFC (1:50) |
Antibody | pERK1 (T202/Y204) Alexa 488 mouse monoclonal | Cell Signaling | Cat # 4374 | Clone: E10 ICFC (1:20) |
Antibody | pAkt (Ser473) AF647 rabbit monoclonal | Cell Signaling | Cat # 2337 | Clone: 193H12 ICFC (1:20) |
Antibody | p4E-BP1 (Thr37/46) rabbit monoclonal AF647 | Cell Signaling | Cat # 5123 | Clone: 236B4 ICFC (1:20) |
Antibody | FITC Mouse monoclonal Anti-Ki-67 | BD Biosciences | Cat # 556026 RRID: AB_396302 | Clone: B56 (1 µl per 107 cells) |
other | Hoechst 33342 Solution | BD Biosciences | Cat # 561908 | See legend to Figure 6 |
Commercial assay, kit | Click-iT Plus OPP Alexa Fluor 488 | Thermo Fisher | Cat # C10456 | Protein Synthesis Assay Kit |
Antibody | APC rat anti-mouse monoclonal TER-119 | BioLegend | Cat # 116211 | Clone: TER119 (1 µl per 107 cells) |
Antibody | PE rat anti-Mouse monoclonal CD71 | BD biosciences | Cat # 561937 | Clone: C2 (1 µl per 107 cells) |
Sequence-based reagent | gRNA 1 | This study | PCR primer | CGCAGTAGACACGCTATCGCCGG |
Sequence-based reagent | gRNA 2 | This study | PCR primer | GTGGGTTGCTGTGTGGATCGGGG |
Sequence- based reagent | F1 | This study | PCR primer | GCACATGCGCACAGAAGT |
Sequence- based reagent | R1 | This study | PCR primer | CGGACTATCTATCCCCACGA |
Sequence-based reagent | F2 | This study | PCR primer | GTACAGCTATCTGCCAGGAA |
Sequence- based reagent | R2 | This study | PCR primer | CGAGGTCGACGGTATCG |
Sequence- based reagent | F3 | This study | PCR primer | CGATACCGTCGACCTCG |
Sequence- based reagent | R3 | This study | PCR primer | GTGCTAGCAACAGAAGGTTC |
Sequence- based reagent | F4 | This study | PCR primer | GTCTCAATACTGTGGGGTGT |
Commercial assay, kit | Cytofix/Cytoperm | BD biosciences | Cat # BDB554714 | Fixation/Permeabilization Solution Kit |
Commercial assay, kit | DNeasy kit | Qiagen | Cat #69504 | DNA extraction kit |
Chemical compound, drug | mSCF | Peprotech | Cat # 250–03 | Cytokine |
Other | MethoCult GF M3434 | Stem Cell Technologies | Cat # 03434 | Methylcellulose media |
Other | MethoCult M3334 | Stem Cell Technologies | Cat # 03334 | Methylcellulose media |
Other | CD117 MicroBeads, mouse | Miltenyi Biotec | Cat #130-091-224 | See ‘Western blot analysis’ in methods section |
Other | LS Column | Miltenyi Biotec | Cat # 130-042-401 | Separation column |
Antibody | RpS12, rabbit polyclonal | Proteintech | Cat # 16490–1-AP | 1:1000 |
Antibody | β-Actin (13E5) Rabbit monoclonal mAb | Cell Signaling | Cat # 4970 | 1:1000 |
Antibody | eIF2α (D7D3) Rabbit monoclonal mAb | Cell Signaling | Cat # 5324 | 1:1000 |
Antibody | phospho-eIF2α (Ser52) rabbit polyclonal | Thermo Scientific | Cat # BS-4842R | 1:1000 |
Commercial assay, kit | miRNA First- Strand Synthesis Kit | Takara Bio | Cat # 638313 | |
Commercial assay, kit | mirVana miRNA Isolation Kit | Thermo Fisher | Cat # AM1560 | |
Other | Power SYBR Green Master Mix | Applied Biosystems | Cat # 4367659 | PCR reaction mix |