TY - JOUR TI - CASP microdomain formation requires cross cell wall stabilization of domains and non-cell autonomous action of LOTR1 AU - Kolbeck, Andreas AU - Marhavý, Peter AU - De Bellis, Damien AU - Li, Baohai AU - Kamiya, Takehiro AU - Fujiwara, Toru AU - Kalmbach, Lothar AU - Geldner, Niko A2 - Benitez-Alfonso, Yoselin A2 - Kleine-Vehn, Jürgen A2 - Jallais, Yvon A2 - Somssich, Marc VL - 11 PY - 2022 DA - 2022/01/14 SP - e69602 C1 - eLife 2022;11:e69602 DO - 10.7554/eLife.69602 UR - https://doi.org/10.7554/eLife.69602 AB - Efficient uptake of nutrients in both animal and plant cells requires tissue-spanning diffusion barriers separating inner tissues from the outer lumen/soil. However, we poorly understand how such contiguous three-dimensional superstructures are formed in plants. Here, we show that correct establishment of the plant Casparian Strip (CS) network relies on local neighbor communication. We show that positioning of Casparian Strip membrane domains (CSDs) is tightly coordinated between neighbors in wild-type and that restriction of domain formation involves the putative extracellular protease LOTR1. Impaired domain restriction in lotr1 leads to fully functional CSDs at ectopic positions, forming ‘half strips’. LOTR1 action in the endodermis requires its expression in the stele. LOTR1 endodermal expression cannot complement, while cortex expression causes a dominant-negative phenotype. Our findings establish LOTR1 as a crucial player in CSD positioning acting in a directional, non-cell-autonomous manner to restrict and coordinate CS positioning. KW - arabidopsis KW - endodermis KW - casparian strip KW - microdomains KW - network KW - neprosin JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -