TY - JOUR TI - Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons AU - Nguyen, Minh Q AU - von Buchholtz, Lars J AU - Reker, Ashlie N AU - Ryba, Nicholas JP AU - Davidson, Steve A2 - Seal, Rebecca A2 - Dulac, Catherine VL - 10 PY - 2021 DA - 2021/11/26 SP - e71752 C1 - eLife 2021;10:e71752 DO - 10.7554/eLife.71752 UR - https://doi.org/10.7554/eLife.71752 AB - Somatosensory neurons with cell bodies in the dorsal root ganglia (DRG) project to the skin, muscles, bones, and viscera to detect touch and temperature as well as to mediate proprioception and many types of interoception. In addition, the somatosensory system conveys the clinically relevant noxious sensations of pain and itch. Here, we used single nuclear transcriptomics to characterize transcriptomic classes of human DRG neurons that detect these diverse types of stimuli. Notably, multiple types of human DRG neurons have transcriptomic features that resemble their mouse counterparts although expression of genes considered important for sensory function often differed between species. More unexpectedly, we identified several transcriptomic classes with no clear equivalent in the other species. This dataset should serve as a valuable resource for the community, for example as means of focusing translational efforts on molecules with conserved expression across species. KW - somatosensation KW - dorsal root ganglia KW - transcriptomics KW - pain KW - itch JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -