TY - JOUR TI - Pan-genome analysis identifies intersecting roles for Pseudomonas specialized metabolites in potato pathogen inhibition AU - Pacheco-Moreno, Alba AU - Stefanato, Francesca L AU - Ford, Jonathan J AU - Trippel, Christine AU - Uszkoreit, Simon AU - Ferrafiat, Laura AU - Grenga, Lucia AU - Dickens, Ruth AU - Kelly, Nathan AU - Kingdon, Alexander DH AU - Ambrosetti, Liana AU - Nepogodiev, Sergey A AU - Findlay, Kim C AU - Cheema, Jitender AU - Trick, Martin AU - Chandra, Govind AU - Tomalin, Graham AU - Malone, Jacob G AU - Truman, Andrew W A2 - Zambrano, MarĂ­a Mercedes A2 - Storz, Gisela VL - 10 PY - 2021 DA - 2021/11/18 SP - e71900 C1 - eLife 2021;10:e71900 DO - 10.7554/eLife.71900 UR - https://doi.org/10.7554/eLife.71900 AB - Agricultural soil harbors a diverse microbiome that can form beneficial relationships with plants, including the inhibition of plant pathogens. Pseudomonas spp. are one of the most abundant bacterial genera in the soil and rhizosphere and play important roles in promoting plant health. However, the genetic determinants of this beneficial activity are only partially understood. Here, we genetically and phenotypically characterize the Pseudomonas fluorescens population in a commercial potato field, where we identify strong correlations between specialized metabolite biosynthesis and antagonism of the potato pathogens Streptomyces scabies and Phytophthora infestans. Genetic and chemical analyses identified hydrogen cyanide and cyclic lipopeptides as key specialized metabolites associated with S. scabies inhibition, which was supported by in planta biocontrol experiments. We show that a single potato field contains a hugely diverse and dynamic population of Pseudomonas bacteria, whose capacity to produce specialized metabolites is shaped both by plant colonization and defined environmental inputs. KW - Pseudomonas KW - potato KW - Streptomyces scabies KW - natural products KW - plant disease KW - biocontrol JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -