TY - JOUR TI - Target-specific control of olfactory bulb periglomerular cells by GABAergic and cholinergic basal forebrain inputs AU - De Saint Jan, Didier A2 - Ding, Jun A2 - Huguenard, John R VL - 11 PY - 2022 DA - 2022/02/28 SP - e71965 C1 - eLife 2022;11:e71965 DO - 10.7554/eLife.71965 UR - https://doi.org/10.7554/eLife.71965 AB - The olfactory bulb (OB), the first relay for odor processing in the brain, receives dense GABAergic and cholinergic long-range projections from basal forebrain (BF) nuclei that provide information about the internal state and behavioral context of the animal. However, the targets, impact, and dynamic of these afferents are still unclear. How BF synaptic inputs modulate activity in diverse subtypes of periglomerular (PG) interneurons using optogenetic stimulation and loose cell-attached or whole-cell patch-clamp recording in OB slices from adult mice were studied in this article. GABAergic BF inputs potently blocked PG cells firing except in a minority of calretinin-expressing cells in which GABA release elicited spiking. Parallel cholinergic projections excited a previously overlooked PG cell subtype via synaptic activation of M1 muscarinic receptors. Low-frequency stimulation of the cholinergic axons drove persistent firing in these PG cells, thereby increasing tonic inhibition in principal neurons. Taken together, these findings suggest that modality-specific BF inputs can orchestrate synaptic inhibition in OB glomeruli using multiple, potentially independent, inhibitory or excitatory target-specific pathways. KW - acetylcholine KW - olfactory bulb KW - basal forebrain KW - GABA KW - periglomerular cells JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -