TY - JOUR TI - α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization AU - Ma, Dandan AU - Wang, Fulin AU - Wang, Rongyi AU - Hu, Yingchun AU - Chen, Zhiquan AU - Huang, Ning AU - Tian, Yonglu AU - Xia, Yuqing AU - Teng, Junlin AU - Chen, Jianguo A2 - Pan, Junmin A2 - Sengupta, Piali A2 - Pan, Junmin VL - 11 PY - 2022 DA - 2022/02/04 SP - e73252 C1 - eLife 2022;11:e73252 DO - 10.7554/eLife.73252 UR - https://doi.org/10.7554/eLife.73252 AB - The centrosome composed of a pair of centrioles (mother and daughter) and pericentriolar material, and is mainly responsible for microtubule nucleation and anchorage in animal cells. The subdistal appendage (SDA) is a centriolar structure located at the mother centriole’s subdistal region, and it functions in microtubule anchorage. However, the molecular composition and detailed structure of the SDA remain largely unknown. Here, we identified α-taxilin and γ-taxilin as new SDA components that form a complex via their coiled-coil domains and that serve as a new subgroup during SDA hierarchical assembly. The taxilins’ SDA localization is dependent on ODF2, and α-taxilin recruits CEP170 to the SDA. Functional analyses suggest that α- and γ-taxilin are responsible for SDA structural integrity and centrosomal microtubule anchorage during interphase and for proper spindle orientation during metaphase. Our results shed light on the molecular components and functional understanding of the SDA hierarchical assembly and microtubule organization. KW - centrosome KW - microtubule KW - super-high resolution JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -