Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptors
A large number of inhibitory receptors recruit SHP1 and/or SHP2, tandem-SH2-containing phosphatases through phosphotyrosine-based motifs immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM). Despite the similarity, these receptors exhibit differential effector binding specificities, as exemplified by the immune checkpoint receptors PD-1 and BTLA, which preferentially recruit SHP2 and SHP1, respectively. The molecular basis by which structurally similar receptors discriminate SHP1 and SHP2 is unclear. Here, we provide evidence that human PD-1 and BTLA optimally bind to SHP1 and SHP2 via a bivalent, parallel mode that involves both SH2 domains of SHP1 or SHP2. PD-1 mainly uses its ITSM to prefer SHP2 over SHP1 via their C-terminal SH2 domains (cSH2): swapping SHP1-cSH2 with SHP2-cSH2 enabled PD-1:SHP1 association in T cells. In contrast, BTLA primarily utilizes its ITIM to prefer SHP1 over SHP2 via their N-terminal SH2 domains (nSH2). The ITIM of PD-1, however, appeared to be de-emphasized due to a glycine at pY+1 position. Substitution of this glycine with alanine, a residue conserved in BTLA and several SHP1-recruiting receptors, was sufficient to induce PD-1:SHP1 interaction in T cells. Finally, structural simulation and mutagenesis screening showed that SHP1 recruitment activity exhibits a bell-shaped dependence on the molecular volume of the pY+1 residue of ITIM. Collectively, we provide a molecular interpretation of the SHP1/SHP2-binding specificities of PD-1 and BTLA, with implications for the mechanisms of a large family of therapeutically relevant receptors.
This study elegantly addressed the SHP1/SHP2 preferences of ITIM/ITSM-containing inhibitory immunoreceptors PD-1 and BTLA, with solid evidence from cell-based, biochemical, biophysical, and domain-swapping assays. Importantly, it lays the foundation for further structural, physiological, and therapeutic studies.https://doi.org/10.7554/eLife.74276.sa0
A wide spectrum of biological functions, including cell growth, survival, proliferation, differentiation, adhesion, migration, and communication, critically depend on tyrosine phosphorylations that occur on both cell surface receptors and intracellular effectors. Phosphotyrosines (pY) interact specifically with Src-homology-2 (SH2) domains to regulate protein-protein interactions and protein conformations (Sadowski et al., 1986; Waksman et al., 1992).
Two sets of enzymes reciprocally control tyrosine phosphorylation: protein tyrosine kinases (PTKs) that catalyze tyrosine phosphorylation and protein tyrosine phosphatases (PTPases) that catalyze the removal of phosphates from pY residues (Denu and Dixon, 1998; Paul and Lombroso, 2003; Senis, 2013; Tonks, 2006). Whereas a subset of PTKs and PTPases are anchored to cell membranes (e.g., receptor tyrosine kinases and receptor-like PTPases), others are cytoplasmic that are recruited by membrane receptors in response to an environmental cue.
SHP1 (PTPN6) and its paralog SHP2 (PTPN11) are cytoplasmic PTPases that are crucial for a wide range of cellular functions. Their dysregulation, due to either mutations or aberrant expression, contributes to a number of human pathologies, particularly cancer (Bard-Chapeau et al., 2011). SHP099, an allosteric inhibitor of SHP2 (Chen et al., 2016), is being evaluated in multiple clinical trials for cancer. SHP1 and SHP2 are structurally similar, both contain tandem-SH2 domains followed by a catalytic domain, and are coexpressed in multiple cell types. However, they are not redundant and contribute to different aspects of cellular functions (Lorenz, 2009; Poole and Jones, 2005). The biochemical basis for these differences is unclear.
Among the many reported functions of SHP1 and SHP2, they are known as key effectors for numerous inhibitory immunoreceptors, which recruit SHP1 and/or SHP2 to repress phosphorylation-dependent stimulatory signaling. These receptors include PD-1, BTLA, and LAIR, which repress the functions of T and B lymphocytes; SIRPα, which inhibits phagocytosis of myeloid cells; KIR/Ly49, which prevent NK cells from killing self-cells; PECAM1 and G6b-B, which inhibit platelet functions (Coxon et al., 2017); as well as several members of Siglecs, Sialic-acid-recognizing receptors. Collectively, these receptors operate as ‘immune checkpoints’ essential for self-tolerance, but can also be subverted by cancers and viruses to escape immune destruction. PD-1 blockade antibodies have produced impressive clinical activity against a subset of human cancer. There is also substantial interest in targeting other inhibitory receptors, or SHP1/SHP2 to overcome resistance to PD-1 targeted therapy (Chen et al., 2020), with promising results in mouse tumor models.
Common to SHP1/SHP2-recruiting immunoreceptors is the presence of one or both types of pY-based motifs in their intracellular domains (ICD): immunoreceptor tyrosine-based inhibitory motif (ITIM, consensus sequence S/I/V/LxYxxI/V/L) (Burshtyn et al., 1996; Daëron et al., 1995) and immunoreceptor tyrosine-based switch motif (ITSM, consensus sequence TxYxxV/I) (Cannons et al., 2011). Once phosphorylated, ITIM and ITSM act as docking sites for the SH2 domains of SHP1/SHP2. Moreover, ITSM in some receptors interacts with SH2-containing adaptor proteins SH2D1A and SH2D1B (Cannons et al., 2011).
Despite the general presumption that ITIM/ITSM-containing receptors recruit both SHP1 and SHP2, increasing evidence suggests that these receptors exhibit differential phosphatase-binding specificities. For example, PD-1 strongly recruits SHP2, but not SHP1, in both T cells and B cells (Okazaki et al., 2001; Yokosuka et al., 2012). In contrast, BTLA prefers to recruit SHP1 over SHP2 (Celis-Gutierrez et al., 2019; Mintz et al., 2019; Xu et al., 2020). These binding preferences were consistent with the functional analyses: deletion of SHP2, but not of SHP1, markedly decreases the inhibitory function of PD-1 in T cells (Xu et al., 2020). In contrast, the inhibitory function of BTLA is severely reduced by SHP1 deletion but not SHP2 deletion from T cells (Xu et al., 2020). Notably, FcγIIRB, another ITIM-containing receptor, recruits neither SHP1 nor SHP2, but recruits and signals through the lipid phosphatase SHIP (Ono et al., 1997).
The distinct phosphatase preferences of PD-1, BTLA, and FcγIIRB are striking and puzzling given the similarities in their pY motifs and in the structures of SHP1/SHP2. This knowledge gap has made it difficult to predict the functions, redundancy, competition, or synergy of the many ITIM-bearing receptors. Addressing these questions has met several challenges. First, both SHP1 and SHP2 can potentially interact with dual phosphorylated receptors in multiple possible modes: monovalent, bivalent parallel, or bivalent antiparallel, etc. Second, there is no reported structure for dual phosphorylated PD-1 or BTLA interacting with SHP1 or SHP2. Third, binding assays using SH2 domains of SHP1/SHP2, as extensively used in previous studies, likely do not reflect the behaviors of full-length proteins in cells. This is because SHP1 and SHP2 undergo complex regulation due to intramolecular contacts (Hof et al., 1998; Pádua et al., 2018; Yang et al., 2003) and reportedly dephosphorylate their docking sites within the receptor (Goyette et al., 2017; Hui et al., 2017; Yokosuka et al., 2012).
In this study, we dissected the molecular mechanisms by which PD-1 and BTLA discriminate between SHP1 and SHP2. We measured the affinities of all potential pY:SH2 interactions involved in PD-1 and BTLA recruitment of SHP1 and SHP2 using surface plasmon resonance (SPR) and identified the optimal binding orientations of both SHP1 and SHP2. We then measured the recruitment of full-length SHP1 and SHP2 to PD-1 microclusters in intact, stimulated T cells expressing similar amounts of either wild-type (WT) or domain-swapped mutant of PD-1. This clean, ‘in-cell’ recruitment assay enabled us to quantitatively measure the net recruitment of SHP1/SHP2 after integrating the various regulatory mechanisms (autoinhibition, autodephosphorylation, etc.), as well as biophysical parameters (avidity, stoichiometry, compartmentalization, etc.). Through these experiments, we identified differing features between SHP1 and SHP2, and between PD-1 and BTLA, that led to the specificity dichotomy. Specifically, we isolated a single residue in ITIM that gates the SHP1-binding activity. Our work sheds light on the effector-binding specificities of a growing list of immune checkpoint receptors.
PD-1 recruits SHP2, but not SHP1, whereas BTLA prefers to recruit SHP1
Previous studies suggest that tyrosine-phosphorylated PD-1 recruits SHP2, but not SHP1, to suppress T cell activation (Xu et al., 2020; Yokosuka et al., 2012). To begin investigating the molecular basis of this specificity, we utilized an antigen-presenting cell (APC) – T cell coculture assay incorporating the PD-L1:PD-1 pathway. In this assay, PD-1-mGFP-transduced Jurkat T cells were stimulated with PD-L1-transduced Raji B cells (APCs) pulsed with superantigen staphylococcal enterotoxin E (SEE). After lysing the cell conjugates at desired time points, we immunoprecipitated (IP) PD-1-mGFP from the cell lysates and probed pY and co-precipitated SHP1 or SHP2 using immunoblots (IB). PD-1 became tyrosine phosphorylated and recruited SHP2, but not SHP1, in a time-dependent fashion (Figure 1A), consistent with prior studies (Xu et al., 2020; Yokosuka et al., 2012). By contrast, in a parallel coculture system containing HVEM-transduced Raji cells and BTLA-mGFP-transduced Jurkat cells, BTLA recruited both SHP1 and SHP2, with a clear preference for SHP1 (Figure 1B), consistent with recent studies (Celis-Gutierrez et al., 2019; Mintz et al., 2019; Xu et al., 2020).
SHP2 deletion, but not SHP1 deletion, decreased the magnitude of anti-PD-1 effect on IL-2 release
The little to no recruitment of SHP1 to PD-1 suggested that SHP1 minimally contributes to PD-1 function. To test this, we determined how deletion of SHP1, SHP2, or both from Jurkat cells affects PD-1 inhibition on IL-2 secretion. To measure the magnitude of PD-1 inhibitory effect, we used pembrolizumab (anti-PD-1) to precisely titrate PD-L1:PD-1 signaling. We created WT, SHP1 KO, SHP2 KO, and SHP1/SHP2 double KO (SHP1/2 DKO) Jurkat cells expressing similar levels of PD-1 (Figure 1—figure supplement 1) and stimulated these cells with SEE-pulsed Raji (PD-L1) cells at increasing concentrations of pembrolizumab. As expected, pembrolizumab dose-dependently increased IL-2 production from PD-1+ WT Jurkat cells (Figure 1C, black). Significantly less pembrolizumab-mediated IL-2 increase was observed in SHP2 KO cells, but not in SHP1 KO cells (Figure 1C, blue and red), arguing that SHP2, but not SHP1, contributes significantly to PD-1 function. Consistent with this notion, the magnitude of pembrolizumab effects was statistically indistinguishable in SHP1/2 DKO cells and in SHP2 KO cells (Figure 1C, green and blue).
Both ITIM and ITSM contribute to the ability of PD-1 to recruit SHP2
We next attempted to clarify the relative contributions of ITIM and ITSM in mediating SHP2 recruitment by examining how mutations of these motifs affect SHP2 binding and PD-1 function. We generated Jurkat cells expressing similar levels of mGFP-tagged PD-1WT, PD-1FY (ITIM Y223 was mutated to phenylalanine), PD-1YF (ITSM Y248 was mutated to phenylalanine), or PD-1FF (both tyrosines were mutated) (Figure 2—figure supplement 1). Upon stimulation with PD-L1-transduced Raji cells, we detected SHP2, but not SHP1, in the PD-1WT IP (GFP IP), and as expected, SHP2 was undetectable in PD-1FF IP samples. The ITSM mutant PD-1YF also failed to recruit SHP2, whereas the ITIM mutant PD-1FY recruited SHP2, but significantly less than PD-1WT (Figure 2A). We confirmed these observations by visualizing PD-1:SHP2 interaction in intact T cells. We plated the foregoing Jurkat cells on a supported lipid bilayer (SLB) containing anti-CD3ε (for T cell receptor [TCR] stimulation) and recombinant PD-L1 ectodomain (PD-L1ECD, for PD-1 stimulation). Total internal reflection microscopy (TIRF-M) in the GFP channel revealed PD-1 microclusters in all four cell types (Figure 2B). Immunostaining of SHP2 showed strong enrichment of SHP2 to PD-1WT microclusters. SHP2 recruitment was slightly weaker for PD-1FY, but statistically significant (p=0.0306). SHP2 recruitment was almost completely abrogated in PD-1YF, similar to the negative control PD-1FF (Figure 2B). These data are in general agreement with previous reports that ITSM is the dominant docking site for SHP2 (Chemnitz et al., 2004; Okazaki et al., 2001; Patsoukis et al., 2020; Peled et al., 2018; Yokosuka et al., 2012). However, our result showed that PD-1WT recruited more SHP2 than did PD-1FY, suggesting that optimal SHP2 recruitment does require ITIM. The more obvious defect of PD-1FY in the co-IP assays might be due to the disruption of weak interactions by the non-equilibrium wash steps.
PD-1-ITSM strongly prefers SHP2-cSH2 over SHP1-cSH2
We next investigated the molecular mechanism by which PD-1 recruits SHP2, but not SHP1, in T cells. SHP1 and SHP2 both contain two SH2 domains in tandem, the N-terminal SH2 (nSH2) and the C-terminal SH2 (cSH2). Our co-IP data indicated that SHP2 interacts with PD-1 in a bivalent fashion involving both SH2 domains. The bivalent interaction can potentially occur either in a parallel fashion in which nSH2 binds to ITIM and cSH2 binds to ITSM, or in an antiparallel fashion in which nSH2 and cSH2 bind to ITSM and ITIM, respectively. To determine the most favorable binding orientation, we next measured the affinities for all the possible pY:SH2 interactions implicated in PD-1:SHP1 and PD-1:SHP2 interactions. We purified pre-phosphorylated ICDs of PD-1 mutants that contained only one tyrosine within either ITIM (PD-1YF) or ITSM (PD-1FY). We then used SPR to measure their binding affinities to purified SHP1-nSH2, SHP1-cSH2, SHP2-nSH2, and SHP2-cSH2 (Figure 3A) and summarized the dissociation constants (Kd) in Supplementary file 1. We also diagramed all the detectable pY:SH2 interactions in the context of tandem SH2 and PD-1WT, with relative affinities depicted by arrow thickness. PD-1-ITSM is a better docking site than is PD-1-ITIM for each of the four SH2 tested (Figure 3B). A careful inspection of the data also revealed specific information, as detailed below.
For PD-1:SHP2 interactions, SHP2-nSH2 weakly preferred PD-1-ITSM (Kd = 0.14 μM) over PD-1-ITIM (Kd = 0.38 μM), and SHP2-cSH2 strongly preferred PD-1-ITSM (Kd = 0.10 μM) over PD-1-ITIM (Kd = 1.4 μM) (Figure 3A and B, Supplementary file 1). Thus, the parallel mode PD-1:SHP2 complex would be more energetically favorable than the antiparallel mode (Figure 3C, right, Supplementary files 2 and 3), consistent with a recent report (Marasco et al., 2020).
For PD-1:SHP1 interactions, SHP1-nSH2 preferred PD-1-ITSM (Kd = 0.083 μM) over PD-1-ITIM (Kd = 0.27 μM). Interestingly, SHP1-cSH2 appeared to be defective in PD-1 binding, exhibiting a rather weak affinity to PD-1-ITSM (Kd = 1.7 μM), and no detectable binding to PD-1-ITIM (Figure 3A and B, Supplementary file 1). The inability of SHP1-cSH2 to bind PD-1-ITIM ruled out the antiparallel mode of PD-1:SHP1 interactions, but indicated the possibility of a monovalent mode in which SHP1-nSH2 interacts with PD-1-ITSM (Figure 3C, left). However, free energy calculations suggested that the parallel mode, which involves two SH2, is energetically favorable over the monovalent mode (Supplementary files 2 and 3).
To further examine the dominant mode of PD-1:SHP1 interactions, we employed a single-molecule assay to determine whether PD-1:SHP1 interactions require only ITSM, as would be expected for the monovalent mode, or both ITIM and ITSM, as would be expected for the bivalent parallel mode. We sparsely attached monomeric, fluorescently labeled, pre-phosphorylated and biotinylated PD-1WT, PD-1FY, or PD-1YF (Figure 3—figure supplement 1A and B) to a biotin polyethylene glycol (PEG)-coated coverslip via streptavidin. TIRF-M resolved individual PD-1 monomers as discrete spots, which underwent photobleaching in single steps (Figure 3—figure supplement 1C and D). After the addition of JF646-labeled tSH2 of either SHP1 or SHP2, we visualized PD-1:tSH2 interaction at the coverslip (Figure 3—figure supplement 1E, left). Recruitment of SHP2-tSH2 to PD-1 led to the appearance of JF646 signal that colocalized with PD-1 molecules (Figure 3—figure supplement 1E, upper right). Each SHP2-tSH2 spot typically persisted for several seconds, then disappeared due to dissociation from PD-1, leading to a step-like time course (Figure 3—figure supplement 1E, lower right). As expected, SHP1-tSH2 displayed a lower degree of PD-1 occupancy (Figure 3—figure supplement 1F). Moreover, mutation of either ITIM (PD-1FY) or ITSM (PD-1YF) strongly reduced the PD-1 occupancy for both SHP1-tSH2 and SHP2-tSH2 as compared to the WT control (PD-1WT) (Figure 3—figure supplement 1F), further supporting that both SHP1-tSH2 and SHP2-tSH2 optimally bind to PD-1WT in a bivalent fashion involving both ITIM and ITSM.
Collectively, data presented in this section demonstrated that both SHP1 and SHP2 interact with PD-1 primarily via the bivalent parallel orientation. However, the PD-1:SHP1 interaction is much less stable due to the very weak affinity between SHP1-cSH2 and PD-1-ITSM.
Replacement of SHP1-cSH2 by SHP2-cSH2 is sufficient to induce PD-1:SHP1 association in T cells
The SPR data (Figure 3B, Supplementary file 1) indicated that the SHP1-cSH2 barely interacts with PD-1-ITSM, whereas SHP2-cSH2 displayed a 17-fold higher affinity to the ITSM of PD-1. Indeed, according to the NMR structure of PD-1-pITSM:SHP2-cSH2 complex (PDB code: 6R5G) (Marasco et al., 2020), multiple residues in SHP2-cSH2 (e.g., K120, M171, T205, T208) that contribute to the interaction with PD-1-ITSM are replaced in SHP1-cSH2 (Figure 4A). Thus, we next determined if swapping the cSH2 of SHP1 with that of SHP2 could induce PD-1:SHP1 binding in T cells. We sought to image the recruitment of domain-swapped chimeric mutants of SHP1 to PD-1 microclusters. To avoid competition from endogenous SHP1 and SHP2, we generated SHP1/2 DKO Jurkat cells and co-transduced PD-1-mGFP with mCherry-tagged SHP1WT or SHP1 mutant with one or both of its SH2 domains replaced by those of SHP2 (Figure 4B, left). Having confirmed that these cells expressed similar levels of PD-1-mGFP and mCherry-tagged SHP1 variants (Figure 4—figure supplement 1), we stimulated each type of Jurkat cells with an SLB containing anti-CD3ε and PD-L1ECD. TIRF-M showed that upon cell-bilayer contact, PD-1 formed microclusters that recruited little to no SHP1WT, as manifested by nearly undetectable mCherry signal in the GFP foci (Figure 4B, top row), consistent with previous reports (Xu et al., 2020; Yokosuka et al., 2012). Swapping the nSH2 of SHP1 with that of SHP2 (SHP1SHP2-nSH2) increased the mCherry signal in PD-1 microclusters, but only to a minor degree (Figure 4B, second row). In contrast, swapping the cSH2 of SHP1 with that of SHP2 (SHP1SHP2-cSH2) led to a marked increase in mCherry signal in the PD-1 microclusters (Figure 4B, third row), to a comparable extent as SHP1SHP2-tSH2, in which both SH2 of SHP1 were replaced with those of SHP2 (Figure 4B, bottom row).
We confirmed the TIRF results using a co-IP assay. After stimulation of the foregoing Jurkat cells (Figure 4B) with PD-L1-transduced Raji cells, we pulled down PD-1-mGFP and examined the co-precipitated SHP1 variants using IB. We detected no signal of SHP1WT, weak signal of SHP1SHP2-nSH2, and strong signal of SHP1SHP2-cSH2 and SHP1SHP2-tSH2 (Figure 4C, GFP IP, SHP1 IB). Notably, PD-1 phosphorylation was inversely correlated with the recruitment of the SHP1 variants (Figure 4C, GFP IP, pY IB), supporting the notion that PD-1 is a substrate for its bound PTPases (Goyette et al., 2017; Hui et al., 2017; Yokosuka et al., 2012). Collectively, data reported in this section demonstrated that cSH2 is the major determinant underlying PD-1’s strong preference for SHP2 over SHP1.
ITIM and ITSM are both required for BTLA to recruit SHP1/SHP2
Having established deficient ITSM:cSH2 interactions as the primary basis for the weak stability of PD-1:SHP1 binding, we next turned our attention to receptors that normally recruit SHP1 in T cells, such as BTLA (Figure 1B), to gain further insights into the mechanisms of effector PTPase discrimination by ITIM/ITSM-bearing receptors (Gavrieli et al., 2003; Mintz et al., 2019; Xu et al., 2020). We wished to determine the structural features in BTLA that enabled its SHP1 recruitment in T cells.
The ICD of human BTLA harbors four phosphorylatable tyrosines (Y226, Y243, Y257, and Y282), in which Y257 and Y282 are embedded in ITIM and ITSM, respectively. We first asked which tyrosine(s) of BTLA are required for SHP1 and SHP2 recruitment. Analogous to PD-1 assays (Figure 2A), we established Jurkat cell lines expressing either WT or mutant BTLA in which one of the four phosphorylatable tyrosines was replaced by phenylalanine (Figure 5A). We then stimulated these cell lines, in parallel, with HVEM-expressing Raji B cells. Co-IP experiments showed that mutation of either or both of the non-ITIM/ITSM phosphorylatable tyrosines (Y226F: BTLAFYYY; Y243F: BTLAYFYY; Y226F and Y243F: BTLAFFYY) had little to no effect on the abilities of BTLA to recruit SHP1/SHP2 (Figure 5, Figure 5—figure supplement 1), demonstrating that these two tyrosines are dispensable for BTLA-mediated recruitment of SHP1/SHP2. In contrast, mutation of either ITIM tyrosine (Y257F: BTLAYYFY) or ITSM tyrosine (Y282F: BTLAYYYF) abolished the binding of both SHP1 and SHP2 (Figure 5B), consistent with previous studies (Gavrieli et al., 2003). Thus, ITIM and ITSM are both necessary for SHP1 and SHP2 recruitment by BTLA. These data also suggest that both SH2 domains of SHP1 and SHP2 are required for their recruitment to BTLA. The more stringent requirement of both ITIM and ITSM of BTLA indicates that its ITIM and ITSM play more balanced roles in mediating SHP1/SHP2 recruitment than those of PD-1.
SHP1 and SHP2 both interact with BTLA via the bivalent parallel mode
We next sought to determine the most favorable binding orientations for BTLA:SHP1 interactions and BTLA:SHP2 interactions. Analogous to SPR assays with PD-1 (Figure 3), we measured the affinities of individual pY:SH2 interaction implicated in BTLA:SHP1/SHP2 interactions using sensor chips coated with pre-phosphorylated BTLA triple-tyrosine-mutant FFYF (BTLA-ITIM), which contained a lone tyrosine (Y257) in its ITIM, or pre-phosphorylated BTLA triple-tyrosine-mutant FFFY (BTLA-ITSM), which contained a lone tyrosine (Y282) in its ITSM (Figure 6A). These experiments revealed that for both SHP1 and SHP2, their nSH2 and cSH2 domains prefer BTLA-ITIM and BTLA-ITSM, respectively (Figure 6A and B, Supplementary file 1). Thus, we concluded that the most favorable BTLA:SHP1 and BTLA:SHP2 interactions both occur in a parallel mode (Figure 6C), similar to PD-1:SHP1 and PD-1:SHP2 interactions.
BTLA-ITIM is a high-affinity docking site for SHP1-nSH2
On a closer inspection of the SPR data, we found that the relative contribution of ITIM and ITSM in BTLA is opposite to that in PD-1. While the ITSM is the major SH2 docking site in PD-1, the ITIM appeared to be the major SH2 docking site in BTLA. This is particularly striking in the case of BTLA:SHP1 interaction: SHP1-nSH2 exhibited an impressive affinity to BTLA-ITIM (Kd = 0.064 μM), 13-fold higher than the affinity between SHP1-cSH2 and BTLA-ITSM (Kd = 0.86 μM) (Figure 6A, Supplementary file 1).
Notably, the affinity of SHP1-nSH2 to BTLA-ITIM (Kd = 0.064 μM) was also four-fold higher than its affinity to PD-1-ITIM (Kd = 0.27 μM) (Figure 6A, Supplementary file 1). Thus, even though BTLA-ITSM is a poor docking site for SHP1-cSH2, akin to PD-1-ITSM, BTLA-ITIM is a much better docking site for SHP1-nSH2 than is PD-1-ITIM. Conceivably, the strong BTLA-ITIM:SHP1-nSH2 interaction may compensate for the weak BTLA-ITSM:SHP1-cSH2 interaction, leading to an overall stable BTLA:SHP1 association in T cells.
Swapping PD-1-ITIM with BTLA-ITIM induced PD-1:SHP1 interaction in T cells
The foregoing data support a hypothesis that the stability of ITIM:SH2 interactions is vital for ITIM/ITSM-bearing receptors to recruit SHP1. To test this experimentally, we assayed whether replacing the ‘low-affinity’ ITIM of PD-1 with the ‘high-affinity’ ITIM of BTLA could induce PD-1:SHP1 interaction. We transduced Jurkat cells with either mGFP-tagged PD-1WT or PD-1BTLA-ITIM, in which we replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYASL) (Figure 7A, Figure 7—figure supplement 1). Following stimulation of both types of Jurkat cells using PD-L1-expressing Raji B cells, co-IP assays revealed that PD-1BTLA-ITIM, but not PD-1WT, recruited SHP1 (Figure 7B). We confirmed this finding using SHP2 KO Jurkat cells, which allowed us to examine PD-1:SHP1 interaction without potential competition from SHP2 (Figure 7A and B). We further verified these findings in intact cells using TIRF-M. In the cell-SLB assays, we observed microclusters of both PD-1WT and PD-1BTLA-ITIM, and confirmed that the PD-1BTLA-ITIM microclusters recruited significantly more SHP1 than did the PD-1WT microclusters (Figure 7C). Finally, in a reciprocal set of experiments, we found that the replacement of the BTLA-ITIM with the PD-1-ITIM markedly decreased the SHP1 recruitment to BTLA in both WT Jurkat and SHP2 KO Jurkat cells (Figure 7—figure supplement 2). Together, data presented in this section demonstrated that SHP1 primarily discriminates BTLA from PD-1 based on their ITIMs.
Replacement of the pY+1 glycine in PD-1-ITIM with alanine was sufficient to induce PD-1:SHP1 association in T cells
We noted that the BTLA-ITIM (IVYASL) differs from PD-1-ITIM (VDYGEL) at four residues flanking pY: V221, D222, G224, and E225 in PD-1-ITIM are replaced by I, V, A, and S, respectively, in BTLA-ITIM (Figure 8A). We wished to determine which replacement contributed the most in inducing SHP1 binding of PD-1BTLA-ITIM. We generated SHP2 KO Jurkat cells expressing comparable levels of PD-1V221I, PD-1D222V, PD-1G224A, or PD-1E225S, each containing a C-terminal mGFP (Figure 8—figure supplement 1A), and asked which mutants were able to recruit SHP1. We also used cells expressing PD-1WT-mGFP and cells expressing PD-1BTLA-ITIM-mGFP as controls. Upon stimulation with PD-L1-transduced Raji cells, we IP’ed PD-1-mGFP and blotted for SHP1. As expected, SHP1 signal was evident in the precipitates of PD-1BTLA-ITIM, but not in PD-1WT. Notably, PD-1G224A recruited the most SHP1 among the single-point mutants, despite less than PD-1BTLA-ITIM, the positive control (Figure 8B). These results suggest that the alanine residue at the pY+1 position of BTLA-ITIM plays a key role in SHP1 recruitment. However, the significantly lower SHP1 recruitment for PD-1G224A than for PD-1BTLA-ITIM (p=0.000016) indicates that other residues within the BTLA-ITIM also contribute to SHP1 binding to some extent, even though V221I, D222V, or E225S point mutation induced little PD-1:SHP1 association.
We validated the aforementioned findings using TIRF imaging of SLB-stimulated T cells. We observed PD-1 microclusters for all six PD-1 variants. Consistent with Figure 7C, microclusters of PD-1BTLA-ITIM, but not PD-1WT, recruited SHP1 (Figure 8C, rows 1 and 2). Among the single-point mutants, only PD-1G224A microclusters clearly recruited SHP1 (Figure 8C, row 5), albeit less than did PD-1BTLA-ITIM microclusters when SHP1 signal was normalized to the PD-1 signal. In contrast, the other three single-point mutants (PD-1V221I, PD-1D222V, and PD-1E225S) showed little to no SHP1 recruitment (Figure 8C, rows 3, 4, and 6).
Indeed, sequence alignment revealed that alanine is conserved at pY+1 position of ITIM in several inhibitory receptors (Figure 8—figure supplement 1B), including Siglec6, Siglec9, CD300LF, VSTM4, and SIRPα, most of which reportedly recruit SHP1 (Alvarez-Errico et al., 2004; Avril et al., 2004; Crocker et al., 2007; Sui et al., 2004; Veillette et al., 1998). As expected, swapping the PD-1-ITIM by the ITIM of the foregoing inhibitory receptors (Figure 8D, Figure 8—figure supplement 1C) significantly increased SHP1 recruitment to PD-1 immunoprecipitates and PD-1 microclusters as compared to PD-1WT (Figure 8E and F).
A medium-sized nonpolar residue at pY+1 position of the ITIM is required for SHP1 recruitment
The ability of the somewhat conservative mutation (G224A) to induce PD-1:SHP1 binding was unexpected; however, given that alanine has a larger side chain than does glycine, we next sought to determine how the side chain property of pY+1 position influences PD-1:SHP1 interaction. We first simulated the structure of PD-1-pITIM:SHP1-nSH2 through homology modeling based on the structure of PD-1-pITIM:SHP2-nSH2 (PDB code: 6ROY) (Marasco et al., 2020). The simulation revealed a hydrophobic pocket within SHP1-nSH2 that likely coordinates the side chain of pY+1 alanine (Figure 9A). Further structural simulation suggests that the size of the hydrophobic pocket is a good fit for a medium-sized nonpolar residue alanine, valine, leucine, or isoleucine, but not for a larger residue phenylalanine or tryptophan (Figure 9B). To test this experimentally, we mutated the glycine to a series of residues that differ in the size and polarity of their side chains. We established Jurkat lines that express each of the PD-1 mutants fused to a GFP tag at comparable levels indicated by flow cytometry (Figure 9—figure supplement 1A). In the cell-SLB assay, we found that a nonpolar residue with a medium-sized side-chain(G224A, G224V, G224L, G224I) but not a bulky or rigid side-chain (G224F, G224W, and G224P) at pY+1 position of PD-1-ITIM strongly promoted PD-1:SHP1 association (Figure 9C, rows 3–9). Indeed, plotting the SHP1 recruitment against the molecular volume of the seven nonpolar residues, excluding the rigid proline, revealed a bell-shaped dependence that peaked at leucine and isoleucine (Figure 9D). Finally, a polar or charged residue at this position of PD-1 failed to induce SHP1 recruitment, as observed for G224S, G224T, G224K, and G224D mutants (Figure 9C, rows 10–13). These results validated the foregoing structural modeling that a hydrophobic pocket in SHP1-nSH2 coordinates the side chain of the pY+1 residue of ITIM (Figure 9B). Finally, to determine whether the induced SHP1 recruitment enhances PD-1 inhibitory function, we compared the pembrolizumab effect on IL-2 release from SHP2 KO Jurkat cells expressing similar levels of PD-1WT, PD-1G224A, PD-1G224V, PD-1G224L, PD-1G224I, PD-1G224F, or PD-1G224W (Figure 9—figure supplement 1B). Upon stimulation of these cells with Raji (PD-L1) cells with or without pembrolizumab, IL-2 ELISA showed that replacing glycine at pY+1 position with alanine, valine, leucine, or isoleucine, but not phenylalanine or tryptophan, significantly enhanced the PD-1-mediated inhibition of IL-2 secretion (Figure 9E). Taken together, these results revealed that a medium-sized nonpolar residue at pY+1 position of the ITIM is optimal for SHP1 recruitment.
SHP1 and SHP2 are key regulators of cell survival, proliferation, differentiation, and migration (Gascoigne et al., 2015; Ke et al., 2007; Kuo et al., 2010; Lorenz, 2009; Paling and Welham, 2002). Coexpressed in hematopoietic cells, they operate as central effectors for inhibitory immunoreceptors that contain ITIM and ITSM. Dissecting the precise mechanism by which these receptors discriminate between SHP1 and SHP2 is required to predict and understand their ‘checkpoint’ functions. In the present work, we combined biophysical, biochemical, and cellular imaging approaches to investigate the specificity dichotomy of PD-1 and BTLA. Our data have revealed distinct properties between the SH2 domains of SHP1 and SHP2, and between the ITIMs of these two checkpoint receptors. We also report that the differential SHP1-binding activities of PD-1 and BTLA can be largely attributed to a single (pY+1) residue of their ITIMs: the polarity and size of this residue gate SHP1 recruitment in T cells.
In human genome, at least 32 receptors contain two or more ITIMs or ITSMs in tandem. Conceivably, the tandem pY motifs favor their bivalent interactions with tSH2 of SHP1 and SHP2. Mathematical modeling predicts that bivalent binding, even contributed by two weak bonds, can produce stable protein complexes due to an avidity effect and a reduction of off-rate of protein complexes (Diestler and Knapp, 2008). In support of this notion, an earlier study showed that mutation of either ITIM or ITSM of BTLA abolishes its association with both SHP1 and SHP2 (Watanabe et al., 2003), a result that we have confirmed in the present study (Figure 5B). The bivalent binding mode appears to be less strict in the case of PD-1 since its ITIM mutant retained the ability to co-IP SHP2 (Chemnitz et al., 2004; Okazaki et al., 2001; Patsoukis et al., 2020; Peled et al., 2018; Yokosuka et al., 2012). A recent study suggested that SHP2 may crosslink two PD-1 molecules at the ITSM (Patsoukis et al., 2020), whereas other studies indicate that PD-1-ITIM contributes to SHP2 recruitment and activation (Marasco et al., 2020; Peled et al., 2018; Yokosuka et al., 2012). In our hands, ITIM mutant recruited significantly less SHP2 than PD-1WT in co-IP, microcluster enrichment, and single-molecule assays, supporting the involvement of ITIM, and the 1:1 bivalent mode as the dominant mode of PD-1:SHP2 interaction (Figure 2, Figure 3—figure supplement 1). In both BTLA and PD-1, the ITIM tyrosine and ITSM tyrosine are separated by 25 residues (Riley, 2009), and similar spacing are found in numerous ITIM/ITSM-containing receptors (Supplementary file 4). This conserved distance may allow for simultaneous engagement of the ITIM and ITSM by the tSH2 of SHP1/SHP2 in the 1:1 bivalent mode. The bivalent binding likely occurs in a sequential fashion, in which the higher-affinity intermolecular contact precedes and converts the low-affinity binding to a pseudo-intramolecular event, as supported by mathematical modeling (Zhou and Gilson, 2009).
Our SPR data revealed certain promiscuities in the binding of their SH2 to ITIM and ITSM (Figures 3 and 6). Theoretically, SHP1 and SHP2 may bind to PD-1 and BTLA in either a parallel or an antiparallel orientation. However, free energy calculations suggest parallel mode as the most stable form for both PD-1 and BTLA (Supplementary file 2), consistent with a recent study on PD-1:SHP2 interaction (Marasco et al., 2020) and an earlier study on SHP2 interaction with PECAM1 (Jackson et al., 1997). To our knowledge, all ITIM/ITSM receptors with the exception of SLAMF5 in human genome contain an ITIM N-terminal to ITSM (Supplementary file 4). This spatial arrangement might allow these receptors to bind SHP1 and SHP2 in the parallel mode. The physiological significance of parallel binding is unclear, but it might increase the molecular reach of the PTPase domain (Clemens et al., 2021; Zhang et al., 2019), or avoid potential steric clash with the plasma membrane.
Extensive genetic and biochemical evidence shows that SHP1 and SHP2 differ in their physiological functions. SHP2 reportedly acts as both a positive and negative regulator, whereas SHP1 is primarily known as a negative regulator in cell signaling (Lorenz, 2009; Poole and Jones, 2005). The biochemical basis of their functional divergence is unknown. The most striking difference between SHP1 and SHP2, based on the current study, are their cSH2 domains: while SHP2-cSH2 binds to PD-1-ITSM with a high affinity, SHP1-cSH2 exhibits weak binding to PD-1-ITSM (Figure 3A and B). This distinction accounts for the undetectable PD-1:SHP1 association in T cells (Xu et al., 2020; Yokosuka et al., 2012), supported by our domain-swapping experiments (Figure 4B and C). Thus, PD-1 prefers SHP2 over SHP1 primarily based upon the stability of ITSM:cSH2 interaction. We speculate, in a more general sense, that the differing cSH2 domains of SHP1 and SHP2 might enable their recruitment to distinct signalosomes, leading to distinct functional outcomes. Indeed, SHP1-cSH2 and SHP2-cSH2 exhibit 51.9% homology in amino acid identities, and based on a recent NMR structure (Marasco et al., 2020), several residues implicated in PD-1-ITSM:SHP2-cSH2 interactions are altered in SHP1-cSH2 (Figure 4A).
Given the very weak affinity of the SHP1-cSH2 domain with the ITSM of both PD-1 and BTLA, we propose that receptors that stably recruit SHP1 must contain a strong docking site for SHP1-nSH2, such as the BTLA-ITIM (Figure 6A and B). The PD-1-ITIM, however, might be too weak to support stable, bivalent PD-1:SHP1 binding. Moreover, because SHP1/SHP2 undergo autoinhibition due to intramolecular (cis) interactions between the nSH2 and the catalytic domain (Pádua et al., 2018; Pei et al., 1994; Yang et al., 2003), a stronger ITIM might also allow the receptor to more efficiently release the autoinhibition of SHP1/SHP2 in trans. This may also help explain the inability of SHP1 to compensate for PD-1 function in SHP2-deficient T cells (Figure 1C; Xu et al., 2020), even though in a subset of Jurkat cells, we were able to detect weak SHP1 enrichment to PD-1 microclusters.
Biochemical research on PD-1 has led to a consensus that ITSM is its primary docking site for SHP2 (Chemnitz et al., 2004; Okazaki et al., 2001; Patsoukis et al., 2020; Peled et al., 2018; Yokosuka et al., 2012). Along this line, the current study shows that PD-1 uses ITSM to prefer SHP2 over SHP1 (Figure 3A and B). Thus, ITSM is the ‘dominant hand’ of PD-1. In contrast, the ‘dominant hand’ of BTLA appears to be its ITIM. Our data show that ITIM of BTLA serves as the primary docking site for SHP1 and allows BTLA to discriminate between SHP1 and SHP2 (Figure 6A and B).
SH2 binding is contributed by both pY and its flanking residues (Kuriyan and Cowburn, 1997; Pawson, 1995). Alanine is conserved at pY+1 position in the ITIMs of numerous immunoreceptors (Figure 8—figure supplement 1B), suggesting its critical role in the physiological functions of these receptors. In this sense, human PD-1 is an interesting exception: its ITIM appears to be the only ITIM that has a glycine at pY+1 position (Figure 8—figure supplement 1B). Our study demonstrates that the pY+1 glycine inhibits SHP1 recruitment, and replacement of glycine to an alanine, valine, leucine, or isoleucine was sufficient to induce PD-1:SHP1 association in T cells, whereas a polar, charged, or bulky residue was significantly less efficient to promote SHP1 binding (Figures 8 and 9). These results suggest that a medium-sized nonpolar residue at pY+1 position is a defining feature of a SHP1-docking site, specifically its nSH2 domain. Consistent with this model, PECAM1, another receptor reported to strongly prefer SHP2 over SHP1 (Jackson et al., 1997; Sagawa et al., 1997), has a polar residue threonine at pY+1 of its ITIM.
How could a medium-sized hydrophobic residue at pY+1 contribute to SH2 binding? Our homology structural modeling reveals a hydrophobic pocket within SHP1-nSH2 that best coordinates a medium-sized nonpolar side chain from the pY+1 position of ITIM (Figure 9A and B). We speculate that similar hydrophobic contacts likely stabilize BTLA-ITIM:SHP1-nSH2 interactions. A glycine at pY+1, as occurs in human PD-1-ITIM, likely impairs such hydrophobic interactions due to its small size or its structural flexibility, whereas a bulky residue (phenylalanine or tryptophan) would inhibit the interaction due to a steric effect. Further structural studies will be needed to definitively test these notions.
Materials and methods
Cell lines and culturesRequest a detailed protocol
Jurkat E6.1 cells were provided by Dr. Arthur Weiss (University of California San Francisco). HEK293T cells and Raji cells were provided by Dr. Ronald Vale (University of California San Francisco). SHP1 KO Jurkat, SHP2 KO Jurkat, SHP1/2 DKO Jurkat, Raji (PD-L1-mCherry), Raji (HVEM-mRuby2), SHP2 KO Raji (PD-L1-mCherry), and SHP2 KO Raji (HVEM-mRuby2) cells were generated in our previous study (Xu et al., 2020). Each gene of interest was introduced into Jurkat cells via lentiviral transduction, as described previously (Xu et al., 2020). Briefly, each cDNA was cloned into a pHR vector backbone, and co-transfected with pMD2.G and psPAX2 packaging plasmids into HEK293T cells using polyethylenimine (PEI, Fisher Scientific, #NC1014320). Virus-containing supernatants were harvested at 60–72 hr post-transfection. WT Jurkat cells, SHP1 KO Jurkat cells, SHP2 KO Jurkat cells, or SHP1/2 DKO Jurkat cells were resuspended with the desired virus supernatant, centrifuged at 35°C, 1000× g for 30 min, and incubated overnight at 37°C 5% CO2 before replacing the virus supernatant with complete RPMI-1640 medium. HEK293T cells were maintained in DMEM medium (Genesee Scientific, #25-501) supplemented with 10% fetal bovine serum (Omega Scientific, #FB-02) and 1% 100× penicillin-streptomycin (GE Healthcare, #SV30010) at 37°C/5% CO2. Jurkat and Raji cells, authenticated by ATCC using short tandem repeats (STR) profiling, were maintained in RPMI-1640 medium (Corning, #10-041CM) supplemented with 10% fetal bovine serum, 100 U/ml of penicillin, and 100 µg/ml of streptomycin at 37°C/5% CO2. The lack of mycoplasma in the cell lines was confirmed using PCR Mycoplasma Detection Kit (Applied Biological Materials Inc, G238). Cells used in the present study were used within 10 passages from thawing.
Recombinant proteinsRequest a detailed protocol
For SPR assays in Figures 3A and 6A, human BTLAICD (aa 190–289) and PD-1ICD (aa 194–288) tyrosine mutants (BTLAFFYF, BTLAFFFY, PD-1FY, PD-1YF) were expressed with an N-terminal His10 tag in Escherichia coli using the pET28A vector and purified using Ni-NTA agarose (Thermo Fisher, #88223) as described (Xu et al., 2020). His10 tagged human PTK Fyn was expressed in the Bac-to-Bac baculovirus system and purified using Ni-NTA agarose. SNAP-SHP1-nSH2, SNAP-SHP1-nSH2, SNAP-SHP2-nSH2, and SNAP-SHP2-cSH2 were expressed with an N-terminal GST tag followed by a PreScission recognition sequence (LEVLFQGP), in E. coli via the pGEX6p-2 vector. For the single-molecule imaging assay in Figure 3—figure supplement 1, all proteins were expressed with an N-terminal GST tag followed by a PreScission recognition sequence (LEVLFQGP), in E. coli via the pGEX6p-2 vector. These included SNAP-SHP1-tSH2, SNAP-SHP2-tSH2, as well as the ICD of PD-1 WT or its tyrosine mutants fused with an N terminal Avi-tag (GLNDIFEAQKIEWHE) and a C-terminal SNAPf tag (Avi-PD-1YY-SNAPf, Avi-PD-1FY-SNAPf, Avi-PD-1YF-SNAPf). All GST fusion proteins were purified using Glutathione Agarose 4B (Gold Biotechnology, #G-250-50), and eluted with HEPES buffered saline (HBS, 50 mM HEPES, 150 mM NaCl, 0.5 mM TCEP [Gold Biotechnology, #TCEP10], pH 7.5) containing 20 units/ml 3C protease to remove the GST tag. After elution, SNAP-SHP1-tSH2 and SNAP-SHP2-tSH2 were further labeled with SNAP ligand-JF646 (Janelia Research Campus [HHMI], #Janelia 2014-013) at 4°C overnight. Avi-PD-1YY-SNAPf, Avi-PD-1FY-SNAPf, and Avi-PD-1YF-SNAPf were further labeled with SNAP ligand-JF549 (Janelia Research Campus [HHMI], #Janelia 2014-013) and biotin in the presence of 1 mM biotin (Sigma-Aldrich, #B4501), 1 μM BirA, and 10 mM ATP (Gold Biotech, #A-081-100) at 4°C overnight. All affinity-purified proteins were subjected to gel filtration chromatography using HBS containing 10% glycerol and 1 mM TCEP. The monomer fractions were pooled, snap frozen, and stored at –80°C in small aliquots. Gel filtration standards (Bio-Rad, #1511901) were run to confirm the sizes of eluted proteins.
Phosphorylation of recombinant PD-1 and BTLA ICD proteinsRequest a detailed protocol
Purified His10-PD-1ICD, His10-BTLAICD, and Avi-PD-1ICD-SNAPf proteins were incubated with 50 nM purified Fyn, 2 mM ATP, and 10 mM Na3VO4 at room temperature (RT) for 6 hr to achieve full phosphorylation, as indicated by the complete shift of electrophoretic mobility on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Monomeric form of these pre-phosphorylated proteins was purified using gel filtration chromatography in HBS containing 10% glycerol and 1 mM TCEP, and stored in –80°C in aliquots.
SHP2 antibody labelingRequest a detailed protocol
For fluorescent staining of SHP2 in T cells, anti-SHP2 was fluorescently labeled using Alexa Fluor 647 NHS ester (Thermo Fisher, #A37573), and unreacted chemicals were removed using Zeba Spin Desalting Columns (Thermo Fisher, #89890) following the manufacturer’s instructions.
Jurkat-Raji coculture assay for immunoprecipitation and IL-2 secretionRequest a detailed protocol
For Figures 1, 2A, 4C, 5B, 7B, 8B and E, Figure 5—figure supplement 1B, Figure 7—figure supplement 2B, Jurkat cells were starved in serum-free RPMI medium at 37°C for 3 hr prior to coculture. Raji cells were preincubated with 30 ng/ml SEE (Toxin Technologies, #ET404) in RPMI medium for 30 min at 37°C. In order to avoid SHP2 competition from Raji cells, SHP2 KO Raji cells were used in Figures 4C, 7B, 8B and E, Figure 7—figure supplement 2B. Afterward, 4 million SEE-loaded Raji cells and 4 million Jurkat cells were precooled on ice and mixed in a 96-well plate. After centrifugation at 300× g for 1 min at 4°C to initiate Raji-Jurkat contact, cells were immediately transferred to a 37°C water bath. At 5 min or the time points indicated in the figures, Raji-Jurkat conjugates were lysed with HBS containing 5% glycerol, detergent (1% NP-40), protease inhibitor (1 mM PMSF), and phosphatase inhibitors (10 mM Na3VO4 and 10 mM NaF). GFP-tagged PD-1 variants or BTLA variants were IP’ed from the lysate using GFP-Trap (Chromotek, #gta-20). Equal fractions of the IP samples were subjected to SDS-PAGE and blotted with indicated antibodies. For IL-2 assays shown in Figures 1C and 9E, Raji B cells were preincubated with 30 ng/ml SEE in RPMI medium for 30 min at 37°C. Jurkat cells were preincubated with indicated concentrations of pembrolizumab or with PBS at RT for 30 min. Afterward, 1 × 105 SEE-pulsed Raji B cells and 2 × 105 pembrolizumab/PBS-conditioned Jurkat T cells were mixed in a 96-well U-bottom plate in triplicate wells, followed by centrifugation at 300× g for 1 min to initiate cell-cell contact. Cultures were then incubated in a 37°C/5% CO2 incubator, 6 hr later, supernatants were harvested and IL-2 levels were measured by an ELISA kit (Thermo Fisher, #88702577). For each replicate of each cell line, the measured IL-2 levels were normalized to the condition with the highest IL-2 level and shown as relative IL-2 levels.
Flow cytometryRequest a detailed protocol
Flow cytometry was conducted in an LSRFortessa cell analyzer (BD Biosciences). Indicated Jurkat cells were washed with PBS and analyzed after staining with PE anti-human BTLA (MIH26), PE anti-human PD-1 (MIH4), or Pacific Blue anti-human PD-1 (EH12.2H7). For Figure 4—figure supplement 1, mCherry levels were measured using a FACSAria cell sorter (BD Biosciences) due to a lack of 561 nm laser in the LSRFortessa cell analyzer. Data were analyzed using FlowJo (FlowJo, LLC).
SLB preparation and functionalizationRequest a detailed protocol
SLBs were formed on glass-bottomed 96-well plate (DOT Scientific Inc, #MGB096-1-2-LG-L). Briefly, the plate was cleaned with 2.5% Hellmanex (Sigma-Aldrich, #Z805939-1EA), etched with 5 N NaOH, and used for SLB formation as previously described (Ahrends et al., 2017). Briefly, small unilamellar vesicles (SUVs) derived from dried lipid film containing 95.5% POPC (Avanti Polar Lipids, #850457C), 2% biotin-DPPE (Avanti Polar Lipids, #870285P), 2% DGS-NTA-Ni (Avanti Polar Lipids, #790404C), and 0.1% PEG 5000-PE (Avanti Polar Lipids, #880230C) were added onto freshly treated plates to form SLBs. The SLBs were rinsed with wash buffer (1× PBS containing 0.1% BSA) and mixed with 1 μg/ml streptavidin, 0.1 nM His-tagged human PD-L1 ECD, and 3 nM His-tagged human ICAM-1 ECD at 37°C for 1 hr. Afterward, the SLBs were rinsed with wash buffer and further incubated with 5 μg/ml biotin anti-human-CD3ε at 37°C for 30 min, followed by three rinses with wash buffer and three rinses with imaging buffer (20 mM HEPES pH 7.5, 137 mM NaCl, 5 mM KCl, 1 mM CaCl2, 2 mM MgCl2, 0.7 mM Na2HPO4, 6 mM D-glucose).
Cell-SLB image acquisition and analysisRequest a detailed protocol
Jurkat cells were resuspended in imaging buffer and overlaid onto freshly formed PD-L1/ICAM/Okt3-functionalized SLBs. After 5 min incubation at 37°C, SLB-bound cells were overlaid with 2% paraformaldehyde (PFA, Fisher Scientific, #50980494), and incubated at RT for 15 min for fixation. SLB-associated, PFA-treated cells were washed with blocking buffer (1× PBS containing 3% BSA), and permeabilized with 1× PBS containing 3% BSA and 0.1% Saponin at RT for 30 min. To observe mCherry-tagged SHP1WT or domain swapping mutants (Figure 4B), cells were directly imaged at both GFP (488 nm) and mCherry (561 nm) channels. To observe endogenous SHP2 (Figure 2B), the permeabilized cells were stained with Alexa-Fluor-647-labeled anti-SHP2 at 4°C for 16 hr, followed by fixation with 4% PFA. To observe endogenous SHP1 (Figures 7C—9C), the permeabilized cells were stained with anti-SHP1 at 4°C for 16 hr, followed by fixation with 4% PFA, and further staining with Alexa-Fluor-568-labeled anti-rabbit IgG at RT for 1 hr and another treatment with 4% PFA. The fluorescent cell images were acquired on a Nikon Eclipse Ti TIRF microscope equipped with a 100× Apo TIRF 1.49 NA objective lens, controlled by the Micro-Manager software (Edelstein et al., 2014). Fiji (Schindelin et al., 2012) was used to quantify the degree of recruitment of SHP1 and SHP2 to PD-1 microclusters. Mask images identifying the area of PD-1 microclusters were generated by applying the ‘subtract background’ command to PD-1 (mGFP) images using the default setting. The fluorescent signals of anti-SHP1, anti-SHP2, and PD-1 (GFP) in the masked overlaid images were measured and used to calculate the anti-SHP1 FI/GFP FI ratio and SHP2/PD-1 ratio for each cell.
SPR assayRequest a detailed protocol
For Figures 3A and 6A, direct interaction between individual SH2 (nSH2 or cSH2) of SHP1 or SHP2 and BTLA ICD, PD-1 ICD tyrosine mutants (BTLAFFYF, BTLAFFFY, PD-1FY, PD-1YF) was monitored by OpenSPR (Nicoya) equipped with Ni sensor chip (Nicoya, #SEN-AU-100-10-NTA). Pre-phosphorylated His10-tagged PD-1 ICD or BTLA ICD was immobilized onto a Ni sensor chip to achieve approximately 1500 RU by following the Ni sensor wizard in OpenSPR software. SH2 of interest was diluted in running buffer (20 mM HEPES, 150 mM NaCl, 5 mM imidazole [Sigma-Aldrich, #I202], 0.05% Tween-20, 10% glycerol, pH 7.5), and injected. The association and dissociation phases of SH2 were monitored at a flow rate of 20 μl/min. The Ni sensor chip was regenerated with 50 mM NaOH before injecting the next SH2. Sensorgrams were analyzed using the ‘Evaluate EC50’ method in TraceDrawer software (Ridgeview Instruments).
Single-molecule imaging assayRequest a detailed protocol
For Figure 3—figure supplement 1, surface-passivated coverslips and slide glasses used in single-molecule imaging assays were prepared and assembled as previously described (Chandradoss et al., 2014). Surface-passivated glass chambers were incubated with blocking buffer (50 mM HEPES, 150 mM NaCl, 0.1% BSA) at RT for 5 min and with 0.5 μM streptavidin in blocking buffer at RT for 5 min, followed by two washes with blocking buffer. The glass chambers were further incubated with 10 pM pre-phosphorylated, biotinylated, JF549-labeled PD-1YY, PD-1FY, or PD-1YF, and incubated at RT for 5 min. The unbound proteins were removed with blocking buffer and JF646-labeled SH2 proteins were injected to the chambers. The fluorescent images were acquired at 20 Hz on a Nikon Eclipse Ti TIRF microscope equipped with a 100× Apo TIRP 1.49 NA objective lens, controlled by the Micro-Manager software (Edelstein et al., 2014).
Fluorescent intensity distribution analysis and photobleaching analysisRequest a detailed protocol
For Figure 3—figure supplement 1C and D, JF646-labeled PD-1 attached on coverslips were illuminated with the 50 mW 488 nm laser for photobleaching observation. The initial positions of fluorescent spots for JF646-labeled PD-1 were determined by the ‘ThunderSTORM’ plugin (Ovesný et al., 2014) in Fiji. The fluorescent intensities at each determined position in initial images were measured and plotted into histogram fit with Gaussian distributions, and those of whole image stacks were measured for fluorescent trajectories.
Fluorescent trajectory analysis and colocalization analysisRequest a detailed protocol
For Figure 3—figure supplement 1D and F, the positions of fluorescent spots for JF549-labeled PD-1 were determined by the ‘ThunderSTORM’ plugin in Fiji. Likewise, the fluorescent movies for JF646-labeled tSH2 proteins were Z-projected to ‘maximum intensity’ and analyzed by the ‘ThunderSTORM’ plugin to determine the positions where tSH2 proteins bound. The positions detected in both JF549 and JF646 channels were determined as spots representing PD-1 molecules that recruited tSH2 protein at any given time during the image acquisition. The fluorescent intensities of JF646 within those areas were measured to fluorescent trajectories of tSH2 proteins. The bound and unbound states of tSH2 proteins in the fluorescent trajectories were estimated with Hidden Markov Model in a custom written Python script (https://github.com/HuiLabUCSD/Xu-and-Masubuchi-et-al-eLife-2021; Masubuchi, 2021; copy archived at swh:1:rev:48368d5a6f69d8e68ffcb1a3fd67a7d50219015f). The colocalization rates of PD-1 and tSH2 protein were calculated by dividing the total number of PD-1 spots by the number of PD-1 spots that recruited SH2 proteins within 0.5 s of image acquisitions.
Structural modeling and simulationRequest a detailed protocol
For Figure 9A and B, structural modeling of SHP1-nSH2 interaction with WT or mutant PD-1-ITIM was generated by homology modeling using the SWISS-Model (Waterhouse et al., 2018) based on the reported complex structure of SHP2-nSH2 and PD-1-ITIM (PDB: 6ROY) (Marasco et al., 2020). Figures 4A, 9A and B were prepared using PyMOL (http://www.pymol.org/).
Quantification and statistical analysisRequest a detailed protocol
Data were shown as mean ± s.d., and the number of replicates is indicated in figure legends. Curve fitting and normalization were performed in GraphPad Prism 8 (GraphPad). Statistical significance was evaluated by either Student’s t-test or two-way ANOVA test (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns, not significant). Data with p<0.05 are considered statistically significant.
All data generated or analysed during this study are included in the manuscript and supporting file.
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Yuting MaReviewing Editor; Suzhou Institute of Systems Medicine, China
Tadatsugu TaniguchiSenior Editor; Institute of Industrial Science, The University of Tokyo, Japan
In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.
[Editors' note: this paper was reviewed by Review Commons.]https://doi.org/10.7554/eLife.74276.sa1
Reviewer #1 (Evidence, reproducibility and clarity (Required)):
This paper explores an interesting problem of SHP1/SHP2 preferences of inhibitory immunoreceptors. The author are quick to point out that many of their individual data points confirm published results at some level, but the power of the paper is in the parallel analysis of both PD1, which is strongly biased towards SHP2 and BTLA, which is biased towards SHP1. This gives them the opportunity to test the predictions of descriptive experiment by making simple mutated receptors with swapped ITIM or ITSM domains.
The work is very well done and generally the authors are quite careful and precise about the language used to describe results, in general.
The results are quite striking in that the find plenty of evidence for transient interaction of SHP1 with PD1 based on the biophysical measurements, but don't detect the interactions in pull down or in "in cell" microcluster recruitment experiments. In describing the pull-downs they discuss the issue of dissociation during washing potentially missing interactions that are taking place. I would prefer that the pull down is fine evidence for binding, but lack of pull down is not evidence for lack of binding. They should double check that this language is consistent. Also, unless something has changed in the microcluster binding experiments, this in situ recruitment of SHP2 to PD1 is only observed or a 2-3 minutes and then can't be detected, the situation for SHP2 becoming the same as it is for SHP1. If the kinetics are different in the cleaner systems that have now developed they should show this in a primary figure as this would be then different when what is reported previously.
We agree with the reviewer that pull down is evidence for binding. Indeed, in most, if not all of our assays, our results with pull down were consistent with those in the microcluster imaging. It does seem though the differences observed in the “in cell” microcluster recruitment experiments are less striking than in the pull down experiments. As suggested by the reviewer, we have checked through the manuscript and ensure the language is accurate and consistent.
In our recent study (PMID: 32437509), we conducted a side-by-side comparison of SHP2 and SHP1 recruitment kinetics to PD-1 in a similar system. Both microcluster imaging and co-IP assays showed that PD-1:SHP2 association lasted at least 10 minutes, whereas PD-1:SHP1 recruitment was nearly undetectable. The duration of PD-1:SHP2 association was in good agreement with Takashi Saito’s finding in CD4+ mouse primary T cells (PMID: 22641383). Regardless the somewhat different kinetics in different studies, SHP2 recruitment was transient, as pointed out by the reviewer. We believe that some other effectors contribute to PD-1 inhibitory signaling. In supportive of this notion, we recently found that PD-1 remains partially inhibitory in CD8+ T cells deficient in both SHP1 and SHP2 (PMID: 32437509).
The gap in this study is lack of any functional analysis. The Jurkat model could be quite useful as they have a relatively clean system for asking if the transient binding of SHP1 to PD1 has any functional impact, which they have not yet followed through on. Does PD-1 recruited SHP2 have any impact on function after the 5 minutes? Furthermore, the authors need to keep in mind that mice deficient in SHP2 respond to anti-PD1 checkpoint therapies (Rota, G., Niogret, C., Dang, A. T., Barros, C. R., Fonta, N. P., Alfei, F., Morgado, L., Zehn, D., Birchmeier, W., Vivier, E., and Guarda, G. (2018). Shp-2 Is Dispensable for Establishing T Cell Exhaustion and for PD-1 Signaling in vivo. Cell Rep, 23(1), 39-49. https://doi.org/10.1016/j.celrep.2018.03.026). This is an important issue to discuss in light the very interesting binding analysis the authors have performed. But I think the functional analysis can be part of a future paper.
We appreciate the reviewer’s comments and suggestions. To address the concern on the functional relevance, we first tested the magnitude of PD-1 mediated IL-2 inhibition in WT, SHP1 KO, SHP2 KO and SHP1/SHP2 double KO Jurkat cells using pembrolizumab, an FDAapproved PD-1 blockade antibody, to titrate PD-1 signaling. This new experiment revealed that deletion of SHP1 from Jurkat cells had little effect on PD-1 mediated suppression of IL-2. In contrast, deletion of SHP2 from Jurkat cells significantly decreased the PD-1 inhibitory effect. These results strongly suggest that the transient binding of SHP1 to PD1 does not contribute significantly to PD-1 signaling. We now show this new data as Figure 1C of the revised manuscript.
Relative IL-2 levels produced by PD-1+ WT, SHP1 KO, SHP2 KO or SHP1/2 DKO Jurkat cells stimulated with PD-L1+ Raji cells in the presence of increasing concentrations of pembrolizumab (0, 0.4, 1.3, 4.4, 13.3, 44, 133, or 267 nM). For each type of Jurkat cells, IL-2 data was normalized to that at the highest pembrolizumab concentration (267 nM, or 40 μg/ml). Error bars are s.d. from three independent experiments. ****P < 0.0001; ns, not significant; two-way ANOVA test.
Moreover, we conducted another functional assay to test whether SHP1 recruitment, induced by mutations at the PD-1-ITIM pY+1 residue, correlates with the abilities of PD-1 mutants to suppress IL-2 production. Through careful titration of lentivirus titer, we were able to establish seven Jurkat cell lines, each expressing similar levels of a PD-1 variant, with a different nonpolar residues at the pY+1 position of ITIM (see Figure S1H). After stimulating these cell lines side-by-side with PD-L1+ Raji B cells, we found a strong correlation between the degree of SHP1 recruitment and the degree of IL2 suppression, see Figure 9E of the revised manuscript. Therefore, our new functional data demonstrate that the pY+1 residue of ITIM gates SHP1 recruitment, but also influence PD-1 inhibitory function.
We also agree with this reviewer that our manuscript would benefit from discussion of functional analysis. To this end, we have rewritten both the introduction and Discussion section extensively to discuss more on the physiological and functional relevance.
Speaking of the SHP1/SHP2 recruitment kinetics, as the reviewer alluded to, we did observe SHP2 dissociation from PD-1 after 10 minutes, as seen by Saito and colleagues (PMID: 22641383), as well as our recent study (PMID: 32437509). On the other hand, there is also clear evidence that SHP2 is required for long term inhibitory effect of PD-1. For example, when we deleted SHP2 from Jurkat cells, the ability of PD-1 to suppress IL-2 production at 6-hour time point was decreased by >50%, see Figure 1C of the revised manuscript. We observed a similar effect at even 24 hours (data not shown). These data demonstrate the importance of SHP2 for sustained PD-1 mediated inhibition, even though the PD-1:SHP2 interaction is transient. It is likely that a transient interaction at the receptor level can translate to longer term downstream effects, perhaps by modulating the SHP2 activity. Notably, similar transient interactions have also been reported for TCR and ZAP70 (PMIDs: 12356870, 27869819).
We agree that the SHP2 KO phenotype reported by Rota et al. is interesting, and suggested that Shp2 is not the sole effector for PD-1 inhibitory function in vivo. Consistent with this notion, our recent cell-based study (PMID: 32437509) also suggested the existence of SHP2-independent function of PD-1. However, while it is straightforward to assume that SHP1 mediates the SHP2-independent function of PD-1, our functional experiments using SHP1 KO and SHP1/2 double KO T cells did not support this view (see Figure 1C of the revised manuscript). These results suggest that the SHP2-independent function of PD-1 is mediated by yet-to-be identified effectors. Nevertheless, the existence of SHP2-independent functions of PD-1 does not argue against the contribution of SHP2 in the PD-1 pathway, as has been demonstrated by a number of independent studies.
I would suggest that the title be modified slightly from "SHP1/SHP2 discrimination" to "differential SHP1/SHP2 interaction" and leave discussion of discrimination until they have the functional data integrated over times that are relevant to T cell transcriptional regulation (1-2 hrs). The functional analysis can be in another paper, but it would be interesting to have a paragraph in the discussion raising the outstanding issues beyond stable binding detected by the pull-down and microcluster recruitment experiments- what are the implications for function. Could the transient interactions in the noise of the steady state and equilibrium measurements be functional?
We thank the reviewer for the suggestion. We have changed the title to “Molecular Features Underlying Differential SHP1/SHP2 Binding of Immune Checkpoint Receptors”.
With regards to the discussion whether transient interactions in the noise of the steady state can be functional, it does not seem to be the case for PD-1:SHP1 interactions, as supported by the SHP1 and SHP2 KO experiments in this study and our previous study (PMID: 32437509). From a biochemistry perspective, we feel that even SHP1 is weakly recruited by PD-1, as we observed in a subset of cells, the ‘crappy’ ITIM would not be able to efficiently release the autoinhibition of SHP1. We now add these considerations in the Discussion section of the revised manuscript (lines 397-400).
I would summarise that the work is outstanding as biochemistry and biophysics and it should be published nearly as is. I'm suggesting minor revisions in that the changes are just to text, but I think this is important and somewhat nuanced aspect of the paper that will make it even more helpful to readers.
We appreciate the positive and insightful comments! We have revised the manuscript carefully according to this reviewer’s suggestion.
Reviewer #1 (Significance (Required)):
The authors generate a detailed descriptive data set about the component interaction of SHP1 and SHP2 SH2 domains with PD1 and BTLA intracellular domains. They then test hypotheses generated from the descriptive data set to better define the nature of the interactions and why PD1 recruits primarily SHP2, while BTLA mainly recruits SHP1. PD1 is a major driver or the cancer immunotherapy revolution and SHP2 is the major candidate for a signalling effector of PD1. This paper can become the reference paper for the specificity and engineering of this interaction, which will make it highly significant in a very active and still expanding field.
I still feel that "discrimination" has a functional/activity connotation that is not addressed at all in this paper, but can be addressed. I'm happy to have the suggestion stand and let the authors decide. They need to live with it once its published. Another suggestion- the citations on regulation are mostly old. A good recent paper is Padua, R. A. P., Sun, Y., Marko, I., Pitsawong, W., Stiller, J. B., Otten, R., and Kern, D. (2018). Mechanism of activating mutations and allosteric drug inhibition of the phosphatase SHP2. Nature Communications, 9(1), 4507. https://doi.org/10.1038/s41467-018-06814-w .
We have changed the title to as the reviewer suggested. We believe that the functional questions raised by this reviewer, including the SHP1 and SHP2 contribution in PD-1 signaling, had been addressed in our current study and our recent publication (PMID: 32437509). By measuring a pembrolizumab dose response in WT, SHP1 KO, SHP2 KO, and double KO T cells, we provided evidence that PD-1 inhibitory function is contributed by SHP2, but very little if any by SHP1 (see Figure 1C of the revised manuscript).
We thank this reviewer for suggesting the excellent reference. We have cited this reference in the revised manuscript (lines 86 and 396).
Reviewer #2 (Evidence, reproducibility and clarity (Required)):
In this study, Xu and co-workers investigate the biophysical nature of the interaction between the structurally-related non-transmembrane PTPs Shp1 and Shp2 with the ITIM/ITSMcontaining inhibitory receptors PD-1 and BTLA using cell-based, biochemical, biophysical and domain swapping assays. The primary aim being to better understand how these receptors discriminate between binding Shp1 and/or Shp2, and the orientation of Shp1 and Shp2 engagement. These are major unresolved questions in the field that the authors go some way to addressing in a methodical, rigorous, clear and concise manner. Findings are convincing, correlate well with previous findings and internally, and are complemented with excellent schematics, making it easy to comprehend.
The authors focus primarily on binding affinities to explain differential binding of Shp1 and Shp2 by PD-1 and BTLA ITIMs and ITSMs, but this is only part of the story. Avidity, compartmentalization, stoichiometry of kinases, and relative abundance of Shp1 and Shp2 are also important aspects of the discriminatory mechanism that are not addressed. Competition assays would go some way to addressing the latter point and should at least be considered and discussed.
We agree that various parameters mentioned by this reviewer, such as compartmentalization and relative expression levels would be a concern for a purely cell-free system such as the SPR experiments (Figures 3 and 6), however, we feel that our cell-based assays (Figure 4B and Figures 7-9), already integrate these parameters. This is also precisely the reason why we chose to examine the recruitments of SHP1/2 in a cellular context in conjunction with cell-free systems.
Regarding the competition, we have observed generally consistent results in both WT and SHP2 KO background, with or without the potential competition from endogenous SHP2 (Figure 7B and Figure S4). Our data suggests that competition is not a dominant mechanism for the recruitment specificity we observed.
In the revised manuscript, we have clarified these points in lines 93-96.
Similarly, authors do not address how distortion of the pY binding pocket of Shp1 and Shp2 nSH2 domains in the auto-inhibited conformation is released, allowing the domain to engage with phopho-ITIM/ITSM. Again, this should at least be discussed. Current binding studies do not address this issue.
We feel that the overall recruitment to the PD-1 microclusters as we observed in cells already integrate this auto-inhibition mechanism of SHP1 and SHP2, because we used full length proteins. We do agree with the reviewer that future studies are warranted to address the contributions of each mechanism, including auto-inhibition, concentration, competition, etc., to the overall recruitment. This might require careful and extensive biophysical analyses coupled with mathematical modeling given the complex regulation of SHP1 and SHP2. As suggested by this reviewer, we have discussed the autoinhibition mechanism in multiple locations of the revised manuscript, e.g. lines 95, 395, 397.
Phosphorylation should be indicated in schematic representations in Figures 3, 6 b, c.
We thank the reviewer for this advice, we have now indicated phosphorylation in the revised figure 3 and 6.
Cellular and physiological significance should be further discussed, as well as broader implications of findings to other ITIM/ITSM-containing receptors in other lineages.
We agree with the reviewer that ITIM/ITSM-containing receptors have a much broader implication. As suggested, we have revised the introduction section extensively to put the work in a broader context. For example, we now mention the functions of these receptors in various cell types, including T cells, B cells, NK cells, platelets and cells of the myeloid lineage.
Reviewer #2 (Significance (Required)):
Findings from this study advance our knowledge of how inhibitory checkpoint regulatory receptors discriminate between Shp1 and Shp2, which has important implications for understanding how the unique biochemical, cellular and physiological functions of these receptors and phosphatases are dictated. Indeed, findings lay the foundation for a universal mechanism, that may apply to all ITIM/ITSM receptors in other cell lineages, and perhaps novel ways of targeting these interactions therapeutically.
Compare to existing published knowledge
Although largely correlative with previous studies, findings from this study start to fill major gaps in our knowledge of these biochemical processes, in a highly rigorous, concise and clear manner. Findings from previous studies were more 'piecemeal', whereas this study consolidates and advances important nuances of these interactions. Moreover, it lays the foundation for further structural, physiological and therapeutic studies.
The immune receptor signaling community and beyond, including any lineage in which ITIM/ITSM-containing receptors play a major role in regulating cellular responses.
ITIM/ITSM-containing receptors, kinase-phosphatase molecular switches, cellular reactivity to extracellular matrix proteins
Generally agree with reviewer's comments. Constructive overall and fair. Although I was thinking additional competition experiments, I do not think necessary. Over the top for this study. Hence, 1 month should suffice to revise accordingly.
We thank this reviewer for the constructive and fair comments!
Reviewer #3 (Evidence, reproducibility and clarity (Required)):
Inhibitory immune receptors containing ITIMs function through recruiting the phosphatases SHP-1 and SHP-2. SHP-1 and SHP-2 are remarkably similar yet have different roles in vivo.
How can ITIM-containing immune receptors specifically recruit SHP-1 or SHP-2? In this paper, Xu et al. ask how SHP-1 vs SHP-2 specificity is achieved. They use very thorough biochemical assays to measure the affinity of SHP-1 and SHP-2 for various ITIM/ITSMs and finally pin point some key amino acids that switch an ITIM/ITSM from SHP-2 to SHP-1 specificity. The in vitro biochemical assays are augmented by in cell assays that support their conclusions. Overall, this paper is an incredibly elegant and straight forward paper addressing how SHP-1/SHP-2 specificity is achieved.
Could the western blots in Figure 1 be quantified as the western blots in other figures?
We have quantified the blots in Figure 1 as suggested in the revised manuscript.
The data that the y+1 reside is essential for SHP-1/2 specificity is very convincing. We are curious if the other residues of the ITIM/ITSM also contribute to this specificity, albeit less potently. The PD-1 G224A mutant is still less potent than the PD-1 BTLA ITIM swap, suggesting that while the y+1 position is most important, the other residues contribute some specificity. The authors also included data on a PD-1 variant with the BTLA ITIM A224G mutation (8f), which is slightly better at recruiting SHP-1 than the PD-1 ITIM. It may be worth mentioning this data in the text of the paper as well as displaying it in the figure.
The reviewer raised an excellent point, yes, our data does suggest that other pY-flanking residues within the ITIM also contribute to SHP1 binding. However, the pY+1 residue replacement produced the strongest effect as the reviewer noted. In the revised manuscript, we have acknowledged the potential contributions of other residues.
To further clarify this point, we performed a structural homology modeling of PD-1-pITIM:SHP1nSH2 complex based on the published crystal structure of PD-1-pITIM:SHP2-nSH2 (PDB code: 6ROY) (PMID: 32064351). The simulated structure identified a hydrophobic pocket within SHP1-nSH2 that appeared to bind nonpolar side chain at the pY+1 position. Moreover, the size of the pocket explains why a medium-sized residue would fit the best. These provide a structural interpretation for the important role of the Y+1 position. We now showed the data as Figure 9A, B in the revised manuscript.
A brief introduction to ITIM vs ITSM in the introduction of the paper may be helpful background for readers. For example, ITIM receptors are reasonably well known but how ITSM functionally differs is probably less well known.
We have rewritten the introduction about ITIM and ITSM for better clarity. Specifically, we now mention that some ITSM interacts with both SHP1/2 and SH2-containing adaptor proteins.
Although not the major focus of the paper, broadening out this SHP-1/2 specificity to other immune receptors in the discussion is fascinating. (a) The authors find that a Valine, Leucine, or Isoleucine in place of the Alanine in y+1 is very close to equivalent, yet the A is highly conserved. The authors speculate that there may be an advantage to sub-maximal SHP-1 affinity because it is more easy to regulate. I think this is reasonable speculation but a little unsatisfying given the very small observed difference in SHP-1 binding. If the authors have additional thoughts, I would be interested to hear them.
This reviewer raised an excellent point. We agree that the Valine, Leucine or Isoleucine at the pY+1 position of ITIM did not product an obvious increase in SHP1 recruitment over Alanine at the same position. To further address this concern, we have conducted new functional experiment measuring how the identity of the pY+1 residue affect the ability of PD-1 to inhibit IL-2 production from Jurkat cells. Our result showed that medium sized residues, such as Alanine, Valine, Leucine and Isoleucine, increased the PD-1 potency to a similar extent over the WT PD-1. Therefore, we have removed the questionable discussion about the “sub-maximal SHP1 affinity” in the revised manuscript. That being said, both SHP1 recruitment and IL2 suppression showed a bell shaped dependence on the side chain volume of pY+1 residue, as supported by our new structural modeling data mentioned above. We have make this point clear in the revised manuscript.
(b) The authors note that PD-1 is the only ITIM with a glycine in the Y+1 position. Are there other receptors that function primarily through SHP-2, and how might they achieve this specificity?
Among the several receptors that we tested, PD-1 is the only receptor that exhibited no recruitment of SHP1. The lack of SHP1 recruitment is also true for murine PD-1, which has an acidic glutamate residue at Y+1 position. In addition, earlier work reported that PECAM1 also selectively recruits SHP2, but not SHP1. We have noted that PECAM1 contain a threonine (polar) at the pY+1 position of their ITIMs. Thus, their inability to recruit SHP1 is consistent with our model that a nonpolar residue at Y+1 position is required for strong SHP1 recruitment. We have discussed these points in the revised manuscript.
Figure 9 b Val not Vla, Figure 3a – a legend for the color code may be nice (ie, 20-1000 nM)
We thank the reviewer for catching these, we have fixed the error in Figure 9B and provide the color code in Figure 3A in the revised manuscript.
Reviewer #3 (Significance (Required)):
SHP-1 and SHP-2 play a critical role in regulating immune system function. In addition, the receptors recruiting these phosphatases (like PD-1) are important immunotherapy targets. Previously, the question of SHP-1/SHP-2 specificity has been primarily described for ITIM bearing receptors individually. Other studies have predicted consensus sequences for the tSH2 domains of SHP-1 or SHP-2, but not addressed the defining molecular characteristics of these consensus sites or how these could be combined on ITIM receptors to generate selectivity between these related phosphatases. This paper represents a significant step forward because it provides a unifying mechanism explaining how ITIM-bearing immune receptors specifically recruit SHP-1 or SHP-2. I expect this paper will be broadly interesting to biochemists, immunologists and cancer biologists.
I generally think the other reviewers comments are reasonable and insightful. Together, they suggest no new experiments are necessary. As for the proposed title change, I prefer the authors title and find it to be justified given their data.
Reviewer #4 (Evidence, reproducibility and clarity (Required)):
In this manuscript, Xu and college performed an elaborate study to investigate the molecular basis of Shp1 and Shp2 discrimination by immune checkpoints PD-1 and BTLA. The paper is original, clear, and well written. I only have a few minor comments:
1. Please label the molecular weights to all the western blots/IPs results.
We have labeled the molecular weights to all the blots in the revised manuscript.
2. Please add scale bars to all the microscopy pictures.
We have added scale bars to all the microscopy images in the revised manuscript.
3. For the SPR data, please add the fitting curves.
We thank the reviewer for the suggestion. However, we did not use the fitting curve to calculate the Kd, instead, we plotted the maximum response as a function of concentration to determine the Kd. This is another well accepted method for Kd calculation. In fact, some of the SPR curves fit poorly with the existing algorithm. Thus, showing the fitting curves might distract the readers.
Reviewer #4 (Significance (Required)):
The strength of this paper relies on the details they dissected by using a series of mutagenesis screening experiments, which should be interesting to cell biologists and cancer immunologists.
I think the other reviewer's comments are insightful and constructive, the suggested experiments are necessary and will improve the paper.
We thank this reviewer for the positive comments!https://doi.org/10.7554/eLife.74276.sa2
Article and author information
National Cancer Institute (R37 CA239072-03)
- Enfu Hui
Pew Charitable Trusts
- Enfu Hui
Searle Scholars Program
- Enfu Hui
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
We thank J Wilhelm (UCSD) for sharing the TIRF microscope; P Dennett and J Zhang for critically reading the manuscript. TM is supported by the Human Frontier Science Program postdoctoral fellowship. This work was supported by R37 CA239072 from the National Institute of Health, a Searle Scholar Award from the Kinship Foundation, and a Pew Biomedical Scholar Award from the Pew Charitable Trusts to EH.
- Tadatsugu Taniguchi, Institute of Industrial Science, The University of Tokyo, Japan
- Yuting Ma, Suzhou Institute of Systems Medicine, China
- Received: September 29, 2021
- Accepted: October 16, 2021
- Accepted Manuscript published: November 4, 2021 (version 1)
- Version of Record published: November 30, 2021 (version 2)
© 2021, Xu et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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