TY - JOUR TI - Circadian programming of the ellipsoid body sleep homeostat in Drosophila AU - Andreani, Tomas AU - Rosensweig, Clark AU - Sisobhan, Shiju AU - Ogunlana, Emmanuel AU - Kath, William AU - Allada, Ravi A2 - Sehgal, Amita A2 - VijayRaghavan, K A2 - Jepson, James VL - 11 PY - 2022 DA - 2022/06/23 SP - e74327 C1 - eLife 2022;11:e74327 DO - 10.7554/eLife.74327 UR - https://doi.org/10.7554/eLife.74327 AB - Homeostatic and circadian processes collaborate to appropriately time and consolidate sleep and wake. To understand how these processes are integrated, we scheduled brief sleep deprivation at different times of day in Drosophila and find elevated morning rebound compared to evening. These effects depend on discrete morning and evening clock neurons, independent of their roles in circadian locomotor activity. In the R5 ellipsoid body sleep homeostat, we identified elevated morning expression of activity dependent and presynaptic gene expression as well as the presynaptic protein BRUCHPILOT consistent with regulation by clock circuits. These neurons also display elevated calcium levels in response to sleep loss in the morning, but not the evening consistent with the observed time-dependent sleep rebound. These studies reveal the circuit and molecular mechanisms by which discrete circadian clock neurons program a homeostatic sleep center. KW - circadian KW - sleep homeostasis KW - ellipsoid body KW - Drosophila KW - clock JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -