TY - JOUR TI - Enteroendocrine cell types that drive food reward and aversion AU - Bai, Ling AU - Sivakumar, Nilla AU - Yu, Shenliang AU - Mesgarzadeh, Sheyda AU - Ding, Tom AU - Ly, Truong AU - Corpuz, Timothy V AU - Grove, James CR AU - Jarvie, Brooke C AU - Knight, Zachary A A2 - Chesler, Alexander Theodore A2 - Wassum, Kate M A2 - Chesler, Alexander Theodore VL - 11 PY - 2022 DA - 2022/08/01 SP - e74964 C1 - eLife 2022;11:e74964 DO - 10.7554/eLife.74964 UR - https://doi.org/10.7554/eLife.74964 AB - Animals must learn through experience which foods are nutritious and should be consumed, and which are toxic and should be avoided. Enteroendocrine cells (EECs) are the principal chemosensors in the GI tract, but investigation of their role in behavior has been limited by the difficulty of selectively targeting these cells in vivo. Here, we describe an intersectional genetic approach for manipulating EEC subtypes in behaving mice. We show that multiple EEC subtypes inhibit food intake but have different effects on learning. Conditioned flavor preference is driven by release of cholecystokinin whereas conditioned taste aversion is mediated by serotonin and substance P. These positive and negative valence signals are transmitted by vagal and spinal afferents, respectively. These findings establish a cellular basis for how chemosensing in the gut drives learning about food. KW - physiology KW - appetite KW - feeding JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -