TY - JOUR TI - Telomere length regulation by Rif1 protein from Hansenula polymorpha AU - Malyavko, Alexander N AU - Petrova, Olga A AU - Zvereva, Maria I AU - Polshakov, Vladimir I AU - Dontsova, Olga A A2 - Tyler, Jessica K A2 - Shore, David VL - 11 PY - 2022 DA - 2022/02/07 SP - e75010 C1 - eLife 2022;11:e75010 DO - 10.7554/eLife.75010 UR - https://doi.org/10.7554/eLife.75010 AB - Rif1 is a large multifaceted protein involved in various processes of DNA metabolism – from telomere length regulation and replication to double-strand break repair. The mechanistic details of its action, however, are often poorly understood. Here, we report functional characterization of the Rif1 homologue from methylotrophic thermotolerant budding yeast Hansenula polymorpha DL-1. We show that, similar to other yeast species, H. polymorpha Rif1 suppresses telomerase-dependent telomere elongation. We uncover two novel modes of Rif1 recruitment at H. polymorpha telomeres: via direct DNA binding and through the association with the Ku heterodimer. Both of these modes (at least partially) require the intrinsically disordered N-terminal extension – a region of the protein present exclusively in yeast species. We also demonstrate that Rif1 binds Stn1 and promotes its accumulation at telomeres in H. polymorpha. KW - telomere KW - telomerase KW - Hansenula polymorpha KW - genome stability KW - DNA replication KW - Rif1 JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -