TY - JOUR TI - Exploring the expression patterns of palmitoylating and de-palmitoylating enzymes in the mouse brain using the curated RNA-seq database BrainPalmSeq AU - Wild, Angela R AU - Hogg, Peter W AU - Flibotte, Stephane AU - Nasseri, Glory G AU - Hollman, Rocio B AU - Abazari, Danya AU - Haas, Kurt AU - Bamji, Shernaz X A2 - Toth, Katalin A2 - Pfeffer, Suzanne R VL - 11 PY - 2022 DA - 2022/07/12 SP - e75804 C1 - eLife 2022;11:e75804 DO - 10.7554/eLife.75804 UR - https://doi.org/10.7554/eLife.75804 AB - Protein S-palmitoylation is a reversible post-translational lipid modification that plays a critical role in neuronal development and plasticity, while dysregulated S-palmitoylation underlies a number of severe neurological disorders. Dynamic S-palmitoylation is regulated by a large family of ZDHHC palmitoylating enzymes, their accessory proteins, and a small number of known de-palmitoylating enzymes. Here, we curated and analyzed expression data for the proteins that regulate S-palmitoylation from publicly available RNAseq datasets, providing a comprehensive overview of their distribution in the mouse nervous system. We developed a web-tool that enables interactive visualization of the expression patterns for these proteins in the nervous system (http://brainpalmseq.med.ubc.ca/), and explored this resource to find region and cell-type specific expression patterns that give insight into the function of palmitoylating and de-palmitoylating enzymes in the brain and neurological disorders. We found coordinated expression of ZDHHC enzymes with their accessory proteins, de-palmitoylating enzymes and other brain-expressed genes that included an enrichment of S-palmitoylation substrates. Finally, we utilized ZDHHC expression patterns to predict and validate palmitoylating enzyme-substrate interactions. KW - palmitoylation KW - ZDHHC enzymes KW - RNAseq KW - brain KW - expression patterns KW - database JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -