TY - JOUR TI - Longitudinal analysis of invariant natural killer T cell activation reveals a cMAF-associated transcriptional state of NKT10 cells AU - Kane, Harry AU - LaMarche, Nelson M AU - Ní Scannail, Áine AU - Garza, Amanda E AU - Koay, Hui-Fern AU - Azad, Adiba I AU - Kunkemoeller, Britta AU - Stevens, Brenneth AU - Brenner, Michael B AU - Lynch, Lydia A2 - Wang, Chyung-Ru A2 - Taniguchi, Tadatsugu VL - 11 PY - 2022 DA - 2022/12/02 SP - e76586 C1 - eLife 2022;11:e76586 DO - 10.7554/eLife.76586 UR - https://doi.org/10.7554/eLife.76586 AB - Innate T cells, including CD1d-restricted invariant natural killer T (iNKT) cells, are characterized by their rapid activation in response to non-peptide antigens, such as lipids. While the transcriptional profiles of naive, effector, and memory adaptive T cells have been well studied, less is known about the transcriptional regulation of different iNKT cell activation states. Here, using single-cell RNA-sequencing, we performed longitudinal profiling of activated murine iNKT cells, generating a transcriptomic atlas of iNKT cell activation states. We found that transcriptional signatures of activation are highly conserved among heterogeneous iNKT cell populations, including NKT1, NKT2, and NKT17 subsets, and human iNKT cells. Strikingly, we found that regulatory iNKT cells, such as adipose iNKT cells, undergo blunted activation and display constitutive enrichment of memory-like cMAF+ and KLRG1+ populations. Moreover, we identify a conserved cMAF-associated transcriptional network among NKT10 cells, providing novel insights into the biology of regulatory and antigen-experienced iNKT cells. KW - iNKT cells KW - activation KW - transcriptional remodeling KW - adipose tissue KW - scRNA-Seq KW - cellular metabolism JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -