TY - JOUR TI - Dynamic proteomic and phosphoproteomic atlas of corticostriatal axons in neurodevelopment AU - Dumrongprechachan, Vasin AU - Salisbury, Ryan B AU - Butler, Lindsey AU - MacDonald, Matthew L AU - Kozorovitskiy, Yevgenia A2 - Brose, Nils A2 - Westbrook, Gary L A2 - Poulopoulos, Alexandros VL - 11 PY - 2022 DA - 2022/10/14 SP - e78847 C1 - eLife 2022;11:e78847 DO - 10.7554/eLife.78847 UR - https://doi.org/10.7554/eLife.78847 AB - Mammalian axonal development begins in embryonic stages and continues postnatally. After birth, axonal proteomic landscape changes rapidly, coordinated by transcription, protein turnover, and post-translational modifications. Comprehensive profiling of axonal proteomes across neurodevelopment is limited, with most studies lacking cell-type and neural circuit specificity, resulting in substantial information loss. We create a Cre-dependent APEX2 reporter mouse line and map cell-type-specific proteome of corticostriatal projections across postnatal development. We synthesize analysis frameworks to define temporal patterns of axonal proteome and phosphoproteome, identifying co-regulated proteins and phosphorylations associated with genetic risk for human brain disorders. We discover proline-directed kinases as major developmental regulators. APEX2 transgenic reporter proximity labeling offers flexible strategies for subcellular proteomics with cell type specificity in early neurodevelopment, a critical period for neuropsychiatric disease. KW - neuroproteomics KW - corticostriatal KW - axon KW - development KW - phosphoproteomics JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -