TY - JOUR TI - Brain atlas for glycoprotein hormone receptors at single-transcript level AU - Ryu, Vitaly AU - Gumerova, Anisa AU - Korkmaz, Funda AU - Kang, Seong Su AU - Katsel, Pavel AU - Miyashita, Sari AU - Kannangara, Hasni AU - Cullen, Liam AU - Chan, Pokman AU - Kuo, TanChun AU - Padilla, Ashley AU - Sultana, Farhath AU - Wizman, Soleil A AU - Kramskiy, Natan AU - Zaidi, Samir AU - Kim, Se-Min AU - New, Maria I AU - Rosen, Clifford J AU - Goosens, Ki A AU - Frolinger, Tal AU - Haroutunian, Vahram AU - Ye, Keqiang AU - Lizneva, Daria AU - Davies, Terry F AU - Yuen, Tony AU - Zaidi, Mone A2 - Isales, Carlos A2 - Yang, Yunlei A2 - Huang, Christopher L-H A2 - Wan, Mei VL - 11 PY - 2022 DA - 2022/09/02 SP - e79612 C1 - eLife 2022;11:e79612 DO - 10.7554/eLife.79612 UR - https://doi.org/10.7554/eLife.79612 AB - There is increasing evidence that anterior pituitary hormones, traditionally thought to have unitary functions in regulating single endocrine targets, act on multiple somatic tissues, such as bone, fat, and liver. There is also emerging evidence for anterior pituitary hormone action on brain receptors in mediating central neural and peripheral somatic functions. Here, we have created the most comprehensive neuroanatomical atlas on the expression of TSHR, LHCGR, and FSHR. We have used RNAscope, a technology that allows the detection of mRNA at single-transcript level, together with protein level validation, to document Tshr expression in 173 and Fshr expression in 353 brain regions, nuclei and subnuclei identified using the Atlas for the Mouse Brain in Stereotaxic Coordinates. We also identified Lhcgr transcripts in 401 brain regions, nuclei and subnuclei. Complementarily, we used ViewRNA, another single-transcript detection technology, to establish the expression of FSHR in human brain samples, where transcripts were co-localized in MALAT1-positive neurons. In addition, we show high expression for all three receptors in the ventricular region—with yet unknown functions. Intriguingly, Tshr and Fshr expression in the ependymal layer of the third ventricle was similar to that of the thyroid follicular cells and testicular Sertoli cells, respectively. In contrast, Fshr was localized to NeuN-positive neurons in the granular layer of the dentate gyrus in murine and human brain—both are Alzheimer’s disease-vulnerable regions. Our atlas thus provides a vital resource for scientists to explore the link between the stimulation or inactivation of brain glycoprotein hormone receptors on somatic function. New actionable pathways for human disease may be unmasked through further studies. KW - expression profiling KW - follicle-stimulating hormone KW - thyroid-stimulating hormone KW - luteinizing hormone KW - gonadotropin receptors JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -