TY - JOUR TI - Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the brain AU - Kovacs, Michael A AU - Cowan, Maureen N AU - Babcock, Isaac W AU - Sibley, Lydia A AU - Still, Katherine AU - Batista, Samantha J AU - Labuzan, Sydney A AU - Sethi, Ish AU - Harris, Tajie H A2 - Ginhoux, Florent A2 - Diamond, Betty A2 - Mundt, Sarah VL - 11 PY - 2022 DA - 2022/12/21 SP - e80775 C1 - eLife 2022;11:e80775 DO - 10.7554/eLife.80775 UR - https://doi.org/10.7554/eLife.80775 AB - The discovery of meningeal lymphatic vessels that drain the CNS has prompted new insights into how immune responses develop in the brain. In this study, we examined how T cell responses against CNS-derived antigen develop in the context of infection. We found that meningeal lymphatic drainage promotes CD4+ and CD8+ T cell responses against the neurotropic parasite Toxoplasma gondii in mice, and we observed changes in the dendritic cell compartment of the dural meninges that may support this process. Indeed, we found that mice chronically, but not acutely, infected with T. gondii exhibited a significant expansion and activation of type 1 and type 2 conventional dendritic cells (cDC) in the dural meninges. cDC1s and cDC2s were both capable of sampling cerebrospinal fluid (CSF)-derived protein and were found to harbor processed CSF-derived protein in the draining deep cervical lymph nodes. Disrupting meningeal lymphatic drainage via ligation surgery led to a reduction in CD103+ cDC1 and cDC2 number in the deep cervical lymph nodes and caused an impairment in cDC1 and cDC2 maturation. Concomitantly, lymphatic vessel ligation impaired CD4+ and CD8+ T cell activation, proliferation, and IFN-γ production at this site. Surprisingly, however, parasite-specific T cell responses in the brain remained intact following ligation, which may be due to concurrent activation of T cells at non-CNS-draining sites during chronic infection. Collectively, our work reveals that CNS lymphatic drainage supports the development of peripheral T cell responses against T. gondii but remains dispensable for immune protection of the brain. KW - Toxoplasma gondii KW - neuroimmunology KW - lymphatics KW - T cells JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -