TY - JOUR TI - Osteocytes regulate senescence of bone and bone marrow AU - Ding, Peng AU - Gao, Chuan AU - Gao, Youshui AU - Liu, Delin AU - Li, Hao AU - Xu, Jun AU - Chen, Xiaoyi AU - Huang, Yigang AU - Zhang, Changqing AU - Zheng, Minghao AU - Gao, Junjie A2 - Wan, Mei A2 - Isales, Carlos A2 - Jiang, Jean X A2 - Wan, Mei VL - 11 PY - 2022 DA - 2022/10/28 SP - e81480 C1 - eLife 2022;11:e81480 DO - 10.7554/eLife.81480 UR - https://doi.org/10.7554/eLife.81480 AB - The skeletal system contains a series of sophisticated cellular lineages arising from the mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) that determine the homeostasis of bone and bone marrow. Here, we reasoned that osteocyte may exert a function in regulation of these lineage cell specifications and tissue homeostasis. Using a mouse model of conditional deletion of osteocytes by the expression of diphtheria toxin subunit α in dentin matrix protein 1 (DMP1)-positive osteocytes, we demonstrated that partial ablation of DMP1-positive osteocytes caused severe sarcopenia, osteoporosis, and degenerative kyphosis, leading to shorter lifespan in these animals. Osteocytes reduction altered mesenchymal lineage commitment, resulting in impairment of osteogenesis and induction of osteoclastogensis. Single-cell RNA sequencing further revealed that hematopoietic lineage was mobilized toward myeloid lineage differentiation with expanded myeloid progenitors, neutrophils, and monocytes, while the lymphopoiesis was impaired with reduced B cells in the osteocyte ablation mice. The acquisition of a senescence-associated secretory phenotype (SASP) in both osteogenic and myeloid lineage cells was the underlying cause. Together, we showed that osteocytes play critical roles in regulation of lineage cell specifications in bone and bone marrow through mediation of senescence. KW - osteocyte KW - bone KW - bone marrow KW - muscle JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -