Early-life experience reorganizes neuromodulatory regulation of stage-specific behavioral responses and individuality dimensions during development
- Faculty of Biology, Technion - Israel Institute of Technology, Israel
Figures
Figure 1 with 1 supplement
Long-term behavioral tracking of C. elegans following early starvation reveals discontinuous behavioral effects across developmental stages.
(A) Multi-camera imaging system allows longitudinal behavioral tracking of multiple individual worms across all stages of development following early L1 starvation and without starvation, under …
Figure 1—figure supplement 1
Development time, behavioral trajectory synchronization, and roaming quantification in starved and unstarved wild-type individuals.
(A) Development time across L1–L4 larval stages within starved and unstarved wild-type (N2) populations (no starvation n = 123, 1-day starvation n = 99, 3-day starvation n = 119, 4-day starvation n …
Figure 2 with 1 supplement
Unsupervised analysis of temporal individuality dimensions across development.
(A) Individual animals are ranked based on their roaming activity in each time window compared to other individuals within the same experiment. (B) Heat-map represents relative rank of all N2 …
Figure 2—figure supplement 1
Principal component analysis (PCA) and behavioral consistency analyses.
(A) PC1–8 vectors across developmental time bins extracted by PCA of a shuffled behavioral rank dataset. (B) Variance explained by each of the first 25 PCs following PCA (blue bars), compared to the …
Figure 3 with 1 supplement
Dopamine buffers long-term behavioral effects during intermediate stages of development.
(A) Roaming and dwelling behavior of cat-2 animals without early starvation (n = 124) and following 1-day (n = 98), 3-day (n = 124), and 4-day starvation (n = 85). Each row indicates the …
Figure 3—figure supplement 1
Development time and roaming quantification in starved and unstarved cat-2 individuals.
(A) Development time across L1–L4 larval stages within starved and unstarved cat-2 populations (no starvation n = 124; 1-day starvation n = 98; 3-day starvation n = 124; 4-day starvation n = 85). (B)…
Figure 4 with 3 supplements
Effects of exogenous dopamine and temporally restricted functions of dopamine receptors across intermediate developmental stages.
(A) Average roaming fraction of unstarved (n = 124), 1-day starved (n = 98), and 1-day starved with exogenous DA (n = 46) cat-2 populations. (B) Average roaming fraction of unstarved (n = 124), …
Figure 4—figure supplement 1
Development time and roaming quantification in unstarved, starved, and starved with exogenous DA cat-2 mutants.
(A) Roaming and dwelling behavior of unstarved (n = 124), 1-day starved (n = 98), 1-day starved with exogenous DA (n = 46), 3-day starved (n = 124), and 3-day starved with exogenous DA (n = 50) cat-2…
Figure 4—figure supplement 2
Development time and roaming quantification in starved and unstarved dopamine receptors mutants.
(A) Roaming and dwelling behavior of single dopamine receptor mutants without early starvation (dop-1 n = 73; dop-2 n = 111; dop-3 n = 82) and following 3 days of early starvation (dop-1 n = 134; dop…
Figure 4—figure supplement 3
Development time and roaming quantification in starved and unstarved dopamine receptors double mutants.
(A) Roaming and dwelling behavior of dop-2;dop-3 animals without early starvation (n = 69) and following 1 day (n = 62) and 3 days (n = 63) of early starvation. Each row indicates the age-normalized …
Figure 5 with 1 supplement
Serotonin affects the level of behavioral sensitivity to early stress during early and late developmental stages.
(A) Roaming and dwelling behavior of tph-1 animals without early starvation (n = 51) and following 1-day (n = 87), 3-day (n = 96), and 4-day starvation (n = 104). Each row indicates the …
Figure 5—figure supplement 1
Development time and roaming quantification in starved and unstarved tph-1 individuals.
(A) Development time across L1–L4 larval stages within starved and unstarved tph-1 populations (no starvation n = 51; 1-day starvation n = 87; 3-day starvation n = 96; 4-day starvation n = 104). (B) …
Figure 6 with 1 supplement
Experience-dependent and neuromodulatory effects on variation within individuality dimensions.
(A) Left: PC1 components in each of the 50 time windows. Bar plots represent inter-individual variation in PC1 individual scores within the wild-type and neuromodulatory mutant populations. p-values …
Figure 6—figure supplement 1
Principal component analysis (PCA) and behavioral consistency analyses in wild-type and neuromodulatory mutants.
(A) Variance explained by each of the first 20 PCs following PCA (blue bars) of the pooled wild-type, tph-1, and cat-2 individuals rank dataset, compared to the variance explained by the first 20 …
