TY - JOUR TI - Global change in brain state during spontaneous and forced walk in Drosophila is composed of combined activity patterns of different neuron classes AU - Aimon, Sophie AU - Cheng, Karen Y AU - Gjorgjieva, Julijana AU - Grunwald Kadow, Ilona C A2 - Clark, Damon A A2 - Desplan, Claude VL - 12 PY - 2023 DA - 2023/04/17 SP - e85202 C1 - eLife 2023;12:e85202 DO - 10.7554/eLife.85202 UR - https://doi.org/10.7554/eLife.85202 AB - Movement-correlated brain activity has been found across species and brain regions. Here, we used fast whole brain lightfield imaging in adult Drosophila to investigate the relationship between walk and brain-wide neuronal activity. We observed a global change in activity that tightly correlated with spontaneous bouts of walk. While imaging specific sets of excitatory, inhibitory, and neuromodulatory neurons highlighted their joint contribution, spatial heterogeneity in walk- and turning-induced activity allowed parsing unique responses from subregions and sometimes individual candidate neurons. For example, previously uncharacterized serotonergic neurons were inhibited during walk. While activity onset in some areas preceded walk onset exclusively in spontaneously walking animals, spontaneous and forced walk elicited similar activity in most brain regions. These data suggest a major contribution of walk and walk-related sensory or proprioceptive information to global activity of all major neuronal classes. KW - whole brain activity KW - serotonin KW - neuromodulators KW - walk KW - PCA/ICA KW - dopamine KW - behavior KW - AVLP KW - spontaneous behavior JF - eLife SN - 2050-084X PB - eLife Sciences Publications, Ltd ER -