Antisense, but not sense, repeat expanded RNAs activate PKR/eIF2α-dependent ISR in C9ORF72 FTD/ALS

Decision letter after peer review:

Thank you for submitting your article "Antisense, but not sense, repeat expanded RNAs activate PKR/1 eIF2α-dependent ISR in C9ORF72 FTD/ALS" for consideration by eLife. Your article has been reviewed by 2 peer reviewers, and the evaluation has been overseen by a Reviewing Editor and Suzanne Pfeffer as the Senior Editor. The following individuals involved in the review of your submission have agreed to reveal their identity: Roy Parker (Reviewer #1); Giovanna Mallucci (Reviewer #2).

The reviewers have discussed their reviews with one another, and the Reviewing Editor has drafted this to help you prepare a revised submission.

Essential revisions:

1) Can the authors add data demonstrating that the sense transcript is or is not produced from their antisense C4G2 cassette as per reviewer comments?

2) Can the authors demonstrate that protein translation is rescued upon PKR knockdown as per reviewer comments?

3) The text would be improved by adding additional insight into the potential mechanism of PKR activation by the antisense strand. The hypothesis that the effect is direct and dependent on the secondary structures formed by the RNA is interesting but, as highlighted by reviewer comments, is not the only potential mechanism underlying the data and is not as clearly supported by the current literature as described. The effect could be indirect, dependent on the expression level of the RNAs, involve TDP-43, etc. Alternatively, the authors could provide data supporting their current hypothesis that the effect is dependent on the structure of the C4G2 RNA while this is likely more fitting for future investigations.

4) Please add more information on the ASOs utilized in the study.

Reviewer #1 (Recommendations for the authors):

1. It is not clear whether the antisense C4G2 cassette used at the beginning of the paper also produces a sense G4C2 transcript. While the authors do demonstrate in the case of the G4C2 sense cassette that the antisense transcript is what causes PKR activation, it would be nice for the readers to know whether the G4C2 sense transcript is made from the C4G2 antisense cassette.

2. I could not find information on the exact sequence of the ASO used. Please provide.

3. There is a disconnect between the text and the data in Figure 6 supplement 1F. I assume it is because of a mislabeling of the figure? Currently, the data suggest that treatment of (G4C2)108RO with BOTH antisense and sense ASOs leads to elevated p-PKR.

4. As the authors mention in the discussion, it has previously been shown that phosphorylated PKR is elevated in C9ORF72 ALS/FTD. However, the authors should also make explicit reference to this work in the Results section when showing the p-PKR brain histology.

5. In the discussion, the authors state that sense G4C2 RNAs form G-quadruplexes. The work from Wang et al., 2019, however, indicate that longer G4C2 likely form hairpin structures.

Reviewer #2 (Recommendations for the authors):

Beautifully written and well-presented overall.

It would be good to show the rescue of protein synthesis reduction by PKRi/knockdown in HEKs as done for all other readouts (Figure 2A-C)

Ultimately, it would be good to know (1) the mechanism by which anti-sense secondary structures activate PKR and (2) the relative contributions of this toxic mechanism and others, but this is for future work.