Main text

Rao C, Frodyma DE, Southekal S, Svoboda RA, Black AR, Guda C, Mizutani T, Clevers H, Johnson KR, Fisher KW, Lewis RE. 2021. KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition. eLife 10:e66608. doi: 10.7554/eLife.66608.

Published 10 May 2021

We were alerted by a PubPeer thread about a partial overlap of two representative images that reveals a duplication affecting two fields in Figure 5A (Lamin β2, HCT116 and SW480) and Figure 5—figure supplement 1C (microscope images N-cad OE: siControl/siEPSTI1).

For Figure 5A, at the error originated from improper cropping of the loading control lamin β2 blot image intended to create a lamin β2 image for the middle panel (SW480). We identified the samples loaded in the respective lanes associated with SW480 and used it generate the corrected images in Figure 5A. We also acknowledge the uneven loading lamin β2 in the samples marked with SW480 Con and KSR1 CRISPR. We quantified the relative density of EPSTI1 with Lamin β2 using the corrected data. The quantification results are provided below. This correction does not alter the conclusions stated in the results.

For Figure 5—figure supplement 1C, we suspect that the duplication occurred unintentionally due to errors during the preparation of the figures. The respective correct images have been identified and used to generate corrected images in Figure 5—figure supplement 1C. This error does not impact the quantifications associated with Figure 5—figure supplement 1C, nor does it affect the conclusions of the manuscript. The inclusion of identical lanes/microscopes images in these figures was an unintentional oversight.

The corrected Figure 5 is shown here:

The originally published Figure 5 is shown for reference:

The corrected Figure 5—figure supplement 1 is shown here:

The originally published Figure 5—figure supplement 1 is shown for reference:

The corrected Key Resources Table entries with revised sequences are shown here:

Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Transfected construct (Homo sapiens)KSR1 sgRNA#1This paperpCAG-SpCas9-GFP-U6-gCR1.1TTGGATGCGCGGCGGGAAAG
Transfected construct (Homo sapiens)KSR1 sgRNA#2This paperpCAG-SpCas9-GFP-U6-gCR1.2CTGACACGGAGATGGAGCGT

The originally published Key Resources Table entries are shown for reference:

Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Transfected construct (Homo sapiens)KSR1 sgRNA#1This paperpCAG-SpCas9-GFP-U6-gCR1.1GTGCCAGAAGAGCATGATTTT
Transfected construct (Homo sapiens)KSR1 sgRNA#2This paperpCAG-SpCas9-GFP-U6-gCR1.2GTGCCAGAAGAGCATGATTTT

We thank the users of PubPeer for alerting us of this issue. The article has been corrected accordingly.

Article and author information

Author details

  1. Danielle E Frodyma

  2. Siddesh Southekal

  3. Robert A Svoboda

  4. Adrian R Black

  5. Chittibabu Guda

  6. Tomohiro Mizutani

  7. Hans Clevers

  8. Keith R Johnson

  9. Kurt W Fisher

Version history

  1. Version of Record published: May 7, 2024 (version 1)

Copyright

© 2024, Rao et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 96
    views
  • 0
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Chaitra Rao
  2. Danielle E Frodyma
  3. Siddesh Southekal
  4. Robert A Svoboda
  5. Adrian R Black
  6. Chittibabu Guda
  7. Tomohiro Mizutani
  8. Hans Clevers
  9. Keith R Johnson
  10. Kurt W Fisher
  11. Robert E Lewis
(2024)
Correction: KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition
eLife 13:e99343.
https://doi.org/10.7554/eLife.99343

Share this article

https://doi.org/10.7554/eLife.99343