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A strategical positioning of resident immune cells are key in determining a tightly controlled immune environment in the different regions of the mouse epididymis that creates unique spatial milieus for sperm maturation and combatting invading pathogens.

Integrating a cryogenic light microscope with a focused ion beam/scanning electron microscope, allows directly targeting of fluorescent structure when preparing a frozen-hydrated lamella, without the need for repositioning or fiducial markers.

Cryo-electron microscopy can be used to detect biomolecules in entire 100- to 250-nm-thick cell slices when using a method to collect montages that prevents radiation damage in areas that have not been imaged yet.

Proteomics uncovers thus far unknown age and sex differences in sciatic nerve and skin of naïve mice, highlighting the importance of adequate age matching and parallel investigation of male and female mice in biomedical studies.

GLMsingle is a publicly available toolbox for analyzing fMRI time-series data that leverages data-driven optimization techniques to improve the accuracy of single-trial BOLD response estimates.

A new tool allows researchers to dive into phylogenetic trees containing millions of nodes using just their web browser.

Anti-gephyrin synthetic protein binders were screened and developed to specifically and robustly detect gephyrin protein and gephyrin-interacting protein partners in the mouse brain, generating a useful tool set for neuroscience.

A guide summarizing the diversity of cell types in the parabrachial nucleus.

In situ X-ray imaging of biological tissue samples in staining solution while they are being processed for electron microscopy reveals novel insights into the diffusion-reaction kinetics, tissue mechanics, and staining quality.

Proteolytic events targeting histones can be harnessed to develop antibodies for neutrophil extracellular traps, improving researchers’ ability to distinguish these chromatin-based structures in human samples.