Browse our Tools and Resources

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    1. Developmental Biology
    2. Genetics and Genomics

    A high-resolution mRNA expression time course of embryonic development in zebrafish

    Richard J White, John E Collins ... Elisabeth M Busch-Nentwich
    Global gene expression analysis defines the transcriptional events during the development of zebrafish from fertilised egg to fully formed organism.
    1. Computational and Systems Biology
    2. Neuroscience

    The formation of the light-sensing compartment of cone photoreceptors coincides with a transcriptional switch

    Janine M Daum, Özkan Keles ... Botond Roska
    Correlating changes in structure and gene expression in cone photoreceptors of mice daily, between birth and eye opening, created a resource that supports research in photoreceptor function, development, transplantation and repair.
    1. Structural Biology and Molecular Biophysics

    Model-based local density sharpening of cryo-EM maps

    Arjen J Jakobi, Matthias Wilmanns, Carsten Sachse
    Local sharpening of cryo-electron microscopy maps using atomic models can increase the interpretability of densities in cases of resolution variation and facilitates model building and refinement.
    1. Neuroscience

    Neuropathological and transcriptomic characteristics of the aged brain

    Jeremy A Miller, Angela Guillozet-Bongaarts ... Ed Lein
    High variability in neuropathology burden and interactions between dementia diagnosis and RNA quality present underappreciated complications when studying dementia in an aged population.
    1. Neuroscience

    Computer assisted detection of axonal bouton structural plasticity in in vivo time-lapse images

    Rohan Gala, Daniel Lebrecht ... Armen Stepanyants
    BoutonAnalyzer software makes it possible to detect axonal boutons and measure their structural plasticity in long-term in vivo imaging experiments.
    1. Cell Biology
    2. Ecology

    Quantitative 3D-imaging for cell biology and ecology of environmental microbial eukaryotes

    Sebastien Colin, Luis Pedro Coelho ... Colomban de Vargas
    Three-dimensional fluorescence imaging of microbial eukaryotes in environmental samples allows accurate automated taxonomic profiling and quantitative data about ultrastructures and interactions of organisms.
    1. Ecology
    2. Epidemiology and Global Health

    Using mobile phones as acoustic sensors for high-throughput mosquito surveillance

    Haripriya Mukundarajan, Felix Jan Hein Hol ... Manu Prakash
    Mobile phones can accurately capture sound recordings from mosquito wingbeats with species-specific frequencies, together with metadata about the recording time, location and conditions, to enable rapid low-cost mosquito surveillance using a citizen-science approach.
    1. Neuroscience

    SynEM, automated synapse detection for connectomics

    Benedikt Staffler, Manuel Berning ... Moritz Helmstaedter
    The detection of chemical synapses in 3-dimensional electron microscopy data has been automated such that synapses in large-scale connectivity maps of the cerebral cortex, connectomes, can be charted without the need for human interaction.
    1. Stem Cells and Regenerative Medicine
    2. Genetics and Genomics

    Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6

    Rasmus O Bak, Daniel P Dever ... Matthew H Porteus
    The CRISPR/Cas9 system can be used with recombinant AAV6 donor delivery to facilitate simultaneous, targeted integration into multiple genetic loci in hematopoietic stem and progrenitor cells.
    1. Neuroscience

    A double-sided microscope to realize whole-ganglion imaging of membrane potential in the medicinal leech

    Yusuke Tomina, Daniel A Wagenaar
    For the first time, action potentials and subthreshold postsynaptic potentials of almost all individual identifiable neurons within a functional unit of the leech nervous system were simultaneously imaged during sensory processing and behavioral generation.