A novel statistical algorithm for mining high-dimensional spike train (count) data for significant spatio-temporal patterns reveals new insights into task and brain area dependent functional organization of neural activity.
Junichi Hachisuka, Kyle M Baumbauer ... Sarah E Ross
A new spinal somatosensory preparation is described which enables fine control of sensory input and the ability to manipulate spinal interneurons while recording from spinal projection neurons.
Lilia Leisle, Rahul Chadda ... Christopher A Ahern
Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
Hanneke Vlaming, Thom M Molenaar ... Fred van Leeuwen
The interrogation of histone modifications on DNA barcodes enables efficient and direct screening for epigenetic regulators in thousands of mutants in parallel.
De novo transcriptome assembly and comprehensive characterization of gene expression in proliferating cells of regeneration-capable flatworm Macrostomum lignano advance this organism as a powerful model for stem cell research.
A toxin is used to introduce an otherwise cell-impermeant fluorophore-antibody (or some thing which is equally specific) to bind to an intracellular protein which allows for super resolution imaging and single particle tracking inside the living cell.
An empirically derived scoring system for identifying optimal communication modules of aptazymes accelerates the development of ligand-responsive RNA genetic switches functional in mammalian cells.
Extensive benchmarking reveals that errors made when manually building models into near-atomic-resolution cryoEM density may automatically be corrected using an improved Rosetta-based structure refinement method.
