Figure 3—figure supplement 2. | RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev–Rev response element complex

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RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev–Rev response element complex

Figure 3—figure supplement 2.

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University of California, San Francisco, United States
Figure 3—figure supplement 2.
Download figureOpen in new tabFigure 3—figure supplement 2. Rev-RNA recognition at the junction site.

(A) Model of IIB40 with a deleted AU bulge from the junction site (orange) superimposed with IIB40 showing that deleting the bulge would result in a steric clash between the RNA in A-form geometry and the second Rev subunit. (B) Gel-shift assay using 32P-labled IIB40 or bulge-deleted RNA and Rev showing an absence of the Rev-dimer-RNA complex with the bulge deleted. (C) Simulated annealing Fo − Fc omit map in green (contoured at 3.0 σ) around the water molecule bridging Arg44 from the second Rev subunit and the RNA junction site.

DOI: http://dx.doi.org/10.7554/eLife.04120.016