Figure 3—figure supplement 3. | RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev–Rev response element complex

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RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev–Rev response element complex

Figure 3—figure supplement 3.

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University of California, San Francisco, United States
Figure 3—figure supplement 3.
Download figureOpen in new tabFigure 3—figure supplement 3. Model for RNA-directed cooperative assembly.

(A) An A-form helix was placed near the junction site to represent the stem IIA helix not present in the crystallized RNA. The second Rev subunit from the crystal structure (green) is oriented to use its higher-order oligomerization surface to recruit a third Rev subunit (yellow) (B) The model generated in (A) was placed within the RRE SAXS envelope (Fang et al., 2013), based on the positioning of sites from the SAXS model. (C) RRE secondary structure from Figure 1B placed within the envelope, indicating how other binding sites might be placed, including the IA site. Figures were generated using UCSF Chimera (Pettersen et al., 2004).

DOI: http://dx.doi.org/10.7554/eLife.04120.017