Figure 4—figure supplement 3. | RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev–Rev response element complex

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RNA-directed remodeling of the HIV-1 protein Rev orchestrates assembly of the Rev–Rev response element complex

Figure 4—figure supplement 3.

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University of California, San Francisco, United States
Figure 4—figure supplement 3.
Download figureOpen in new tabFigure 4—figure supplement 3. RNA FISH and protein immunofluorescence studies.

(A) FISH of total HIV RNA or unspliced Gag RNA showing that there is no observable phenotypic difference between rev in the endogenous locus and rev engineered into the nef locus. (B) Immunofluorescence staining for 3× flag-tagged Rev and HIV p24 capsid protein, showing that although Rev is expressed and localized in the nuclei, p24 production is attenuated to different degrees in the presence of Rev mutants. Nuclei were stained using DAPI (blue). Nef::Rev3xF denotes infection with virus containing c-terminally 3× flag-tagged Rev in the nef locus.

DOI: http://dx.doi.org/10.7554/eLife.04120.013