Figure 2—figure supplement 1. | The export receptor Crm1 forms a dimer to promote nuclear export of HIV RNA

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The export receptor Crm1 forms a dimer to promote nuclear export of HIV RNA

Figure 2—figure supplement 1.

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University of California, San Francisco, United States
Figure 2—figure supplement 1.
Download figureOpen in new tabFigure 2—figure supplement 1. Random conical tilt reconstruction and refinement.

(A) Representative fields from a random conical tilt pair of particles assembled with Rev-RRE, Crm1, and RanGTP. (B) Class averages from untilted particles used for random conical tilt. Particle numbers are inset in each image. Classes six and seven were observed with lower concentrations of sample and resemble partially disassembled complexes. Scale bars show 10 nm. (C) Volumes from each class after random conical tilt refinement show similar features among all classes suggesting the classes are different views of the same particle. The volume from the final reconstruction is shown in (D). (E) Fourier shell correlation curves for each random conical tilt class and the final reconstruction. The boost in Fourier shell correlation when C2 symmetry is applied during refinement confirms the symmetric arrangement of bowl-like densities. (F) Angular distribution from projection matching shows no major gap in Euler space.

DOI: http://dx.doi.org/10.7554/eLife.04121.006