Figure 3—figure supplement 2. | The export receptor Crm1 forms a dimer to promote nuclear export of HIV RNA

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The export receptor Crm1 forms a dimer to promote nuclear export of HIV RNA

Figure 3—figure supplement 2.

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University of California, San Francisco, United States
Figure 3—figure supplement 2.
Download figureOpen in new tabFigure 3—figure supplement 2. Localization of Rev-RRE density.

Representative fields and class averages from negative stain (AB) or cryo-electron micrographs (C) show extra density from Rev-RRE when Rev is tagged with GB1 (A, 794 particles) or the RRE tagged with Streptavidin (B, 383 particles). Extra density from Rev-RRE also is apparent in cryo-EM images of complexes that do not have any additional tag (C, 3262 particles), where the contrast is generated from the particles themselves rather than from uranyl ions that typically obscure nucleic acids. Scale bars show 10 nm.

DOI: http://dx.doi.org/10.7554/eLife.04121.011