Juxtaposition of heterozygosity and homozygosity during meiosis causes reciprocal crossover remodeling via interference
- University of Cambridge, United Kingdom
- University of North Carolina at Chapel Hill, United States
- University of Birmingham, United Kingdom
Abstract
During meiosis homologous chromosomes undergo crossover recombination. Sequence differences between homologs can locally inhibit crossovers. Despite this nucleotide diversity and population-scaled recombination are positively correlated in eukaryote genomes. To investigate interactions between heterozygosity and recombination we crossed Arabidopsis lines carrying fluorescent crossover reporters to 32 diverse accessions and observed hybrids with significantly higher and lower crossovers than homozygotes. Using recombinant populations derived from these crosses we observed that heterozygous regions increase crossovers when juxtaposed with homozygous regions, which reciprocally decrease. Total crossovers measured by chiasmata were unchanged when heterozygosity was varied, consistent with homeostatic control. We tested the effects of heterozygosity in mutants where the balance of interfering and non-interfering crossover repair is altered. Crossover remodeling at homozygosity-heterozygosity junctions requires interference and non-interfering repair is inefficient in heterozygous regions. As a consequence heterozygous regions show stronger crossover interference. Our findings reveal how varying homolog polymorphism patterns can shape meiotic recombination.
Article and author information
Author details
Reviewing Editor
- Detlef Weigel, Max Planck Institute for Developmental Biology, Germany
Version history
- Received: June 17, 2014
- Accepted: March 26, 2015
- Accepted Manuscript published: March 27, 2015 (version 1)
- Version of Record published: April 23, 2015 (version 2)
Copyright
© 2015, Ziolkowski et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Juxtaposition of heterozygosity and homozygosity during meiosis causes reciprocal crossover remodeling via interference
eLife 4:e03708.
https://doi.org/10.7554/eLife.03708
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