Figure 4—figure supplement 2. | Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

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Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

Figure 4—figure supplement 2.

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California Institute of Technology, United States
Figure 4—figure supplement 2.
Download figureOpen in new tabFigure 4—figure supplement 2. Reduced sleep at night following Hcrt overexpression requires the hcrtr.

(A, C) Sleep traces from a single representative experiment; yellow region indicates time of HS during day 6. (B, D) Bar graphs show mean ± s.e.m. percentage change in sleep during night 6 compared to night 5 from four combined experiments. (B) In the absence of the hsp:Hcrt transgene, HS during day 6 has no significant effect on hcrtr−/− larvae compared to hcrtr+/− larvae. (D) The reduced sleep induced by Hcrt overexpression is significantly diminished in hcrtr−/− larvae compared to hcrtr+/− sibling controls. n indicates number of larvae. ***, p < 0.0001 and ns, not significant by one-way ANOVA. We note that zebrafish hcrtr−/− larvae have been shown to exhibit normal baseline locomotor activity and sleep levels (Appelbaum et al., 2009), similar to mouse hcrt and hcrt receptor 2 mutants (Willie et al., 2003; Mochizuki et al., 2011).

DOI: http://dx.doi.org/10.7554/eLife.07000.013