Figure 4—figure supplement 4. | Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

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Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

Figure 4—figure supplement 4.

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California Institute of Technology, United States
Figure 4—figure supplement 4.
Download figureOpen in new tabFigure 4—figure supplement 4. The heat shock promoter is not suppressed in hcrtr or dbh mutants.

Representative images of brains from larvae that do not (A) or do (B) contain the hsp:Hcrt transgene, fixed 30 min after HS, and processed for ISH using a hcrt-specific probe. hcrt overexpression levels are similar for hcrtr+/− (B), hcrtr−/− (B′), dbh+/ (B′′) and dbh−/ (B′′′) animals. Larvae were generated by crossing homozygous to heterozygous mutant animals, and brains were genotyped after imaging. Dark spots in (AA′′′) are pigment cells. Endogenous hcrt expression is not observed in (AA′′′) because ISH development was stopped before endogenous hcrt is detectable.

DOI: http://dx.doi.org/10.7554/eLife.07000.015