Figure 5—figure supplement 1. | Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

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Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

Figure 5—figure supplement 1.

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California Institute of Technology, United States
Figure 5—figure supplement 1.
Download figureOpen in new tabFigure 5—figure supplement 1. Specific expression of ChR2-EYFP in Hcrt neurons and temporal dynamics of hcrt:ChR2-EYFP induced locomotor activity.

(A) Representative image from a Tg(hcrt:ChR2-EYFP) larval brain showing Hcrt (red) and EYFP (green) immunoreactivity, and merged images (yellow). Arrow indicates a neuron that is shown at higher magnification in the inset images. Schematic diagram indicates the brain region shown in the images. a = anterior, p = posterior. Scale bar = 20 μm. (B). Activation of Hcrt neurons using ChR2 changes the dynamics of locomotor activity. Tg(hcrt:ChR2-EYFP) larvae reach a higher maximum level of locomotor activity (B), and they do so faster (C), than their non-transgenic siblings. ‘A’ is the maximum activity reached for larvae of a particular genotype and ‘TA’ is the time taken to reach maximum activity, as diagrammed in Figure 5. Data represent mean ± s.e.m. n indicates number of larvae. *, p < 0.05 by one-way ANOVA.

DOI: http://dx.doi.org/10.7554/eLife.07000.017